![Immunohistochemistry-Paraffin: VASA Antibody [NBP2-95280] - Immunochemical staining VASA in human testis with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize2/VASA-Antibody-Immunohistochemistry-Paraffin-NBP2-95280-img0003.jpg "Immunohistochemistry-Paraffin: VASA Antibody [NBP2-95280] - Immunochemical staining VASA in human testis with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).")
| Reactivity | Hu, Rt, Hu, MuSpecies Glossary |
| Applications | IHC, IHC-P |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Produced in rabbits immunized with A synthetic peptide corresponding to the C-terminus of the human VASA. |
| Predicted Species | Human (100%), Mouse (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | DDX4 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.2 um filtered solution in PBS |
| Preservative | No Preservative |
| Purity | Antigen Affinity-purified |
![Neurophysin II/Arg-vasopressin Antibody [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Neurophysin II/Arg-vasopressin Antibody [Unconjugated]")
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | NBP2-95280AF350 | |
| Alexa Fluor 405 | NBP2-95280AF405 | |
| Alexa Fluor 488 | NBP2-95280AF488 | |
| Alexa Fluor 532 | NBP2-95280AF532 | |
| Alexa Fluor 594 | NBP2-95280AF594 | |
| Alexa Fluor 647 | NBP2-95280AF647 | |
| Alexa Fluor 700 | NBP2-95280AF700 | |
| Alexa Fluor 750 | NBP2-95280AF750 | |
| Biotin | NBP2-95280B | |
| DyLight 350 | NBP2-95280UV | |
| DyLight 405 | NBP2-95280V | |
| DyLight 488 | NBP2-95280G | |
| DyLight 550 | NBP2-95280R | |
| DyLight 594 | NBP2-95280DL594 | |
| DyLight 650 | NBP2-95280C | |
| DyLight 680 | NBP2-95280FR | |
| DyLight 755 | NBP2-95280IR | |
| FITC | NBP2-95280F | |
| HRP | NBP2-95280H | |
| Janelia Fluor 549 | NBP2-95280JF549 | |
| Janelia Fluor 646 | NBP2-95280JF646 |
Diseases for VASA Antibody (NBP2-95280)Discover more about diseases related to VASA Antibody (NBP2-95280).
| Pathways for VASA Antibody (NBP2-95280)View related products by pathway.
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PTMs for VASA Antibody (NBP2-95280)Learn more about PTMs related to VASA Antibody (NBP2-95280).
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| Gene Symbol | DDX4 |
![Immunohistochemistry-Paraffin: VASA Antibody [NBP2-95280] - Staining DDX4 in human testis with rabbit polyclonal antibody (1:2000).](http://images.novusbio.com/fullsize2/VASA-Antibody-Immunohistochemistry-Paraffin-NBP2-95280-img0001.jpg "Immunohistochemistry-Paraffin: VASA Antibody [NBP2-95280] - Staining DDX4 in human testis with rabbit polyclonal antibody (1:2000).")
![Immunohistochemistry-Paraffin: VASA Antibody [NBP2-95280] - Staining DDX4 in rat testis with rabbit polyclonal antibody at 1:2000 dilution.](http://images.novusbio.com/fullsize2/VASA-Antibody-Immunohistochemistry-Paraffin-NBP2-95280-img0002.jpg "Immunohistochemistry-Paraffin: VASA Antibody [NBP2-95280] - Staining DDX4 in rat testis with rabbit polyclonal antibody at 1:2000 dilution.")
![Western Blot: OCT4 Antibody [NB100-2379] - YM155 significantly reduced tumorsphere formation and inhibited EGFR autophosphorylation and G9a expression. YM155 is reported to suppress cancer stemness; therefore, we used this compound to investigate the cellular mechanism of tumorsphere formation. To compare the cytotoxic capacity of YM155 against tumorsphere formation and parental cell lines, YM155 was applied to HCC827 and A549 cells in the stemness cultured or integrated medium. (F) Moreover, YM155 inhibited EGFR autophosphorylation in A549-derived tumorspheres and reduced Oct4 expression. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0182149) licensed under a CC-BY licence.](http://images.novusbio.com/images2/OCT4-Antibody-Western-Blot-NB100-2379-img0014.jpg)
![Western Blot: OCT4 Antibody [NB100-2379] - WB detection of OCT4 in mouse brain lysate using OCT4 antibody with ECL based detection. The antibody generated a specific band of OCT4 at the expected ~40kDa position.](http://images.novusbio.com/images2/OCT4-Antibody-Western-Blot-NB100-2379-img0011.jpg)
![Flow Cytometry: OCT4 Antibody [NB100-2379] - An intracellular stain was performed on Jurkat with NB100-2379 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.](http://images.novusbio.com/images2/OCT4-Antibody-Flow-Cytometry-NB100-2379-img0012.jpg)
![Western Blot: Angiotensin II/III Antibody (Ang II E7 (BGN/KA/4L)) [NB100-62346] - Protein expressions of intrarenal RAS components in two groups of mice. Western blot analysis for protein expressions of intrarenal RAS components in the two groups of mice. Image collected and cropped by CiteAb from the following publication (lipidworld.biomedcentral.com/articles/10.1186/1476-511X-12-49), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Angiotensin-II-III-Antibody-(Ang-II-E7-(BGN-KA-4L))-Western-Blot-NB100-62346-img0002.jpg)
![Immunohistochemistry: Angiotensin II/III Antibody (Ang II E7 (BGN/KA/4L)) [NB100-62346] - Protein expressions of intrarenal RAS components in two groups of mice. (A) Immunohistochemical analysis of intrarenal RAS expression position in the two groups of mice. AGT immunoreactivity in the proximal tubular cells, Ang II immunoreactivity in both glomerular and tubular cells, renin immunoreactivity in juxtaglomerular apparatus cells, ACE immunoreactivity in brush border membranes of proximal tubules as well as AT1 and AT2 immunoreactivity in the proximal tubules were increased in the HF group when compared to the control group. Image collected and cropped by CiteAb from the following publication (lipidworld.biomedcentral.com/articles/10.1186/1476-511X-12-49), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Angiotensin-II-III-Antibody-(Ang-II-E7-(BGN-KA-4L))-Immunohistochemistry-NB100-62346-img0003.jpg)
![Western Blot: Angiotensin II/III Antibody (Ang II E7 (BGN/KA/4L)) [NB100-62346] - Evaluation of angiotensis II expression in untreated UUO rat kidneys and UUO rat kidneys treated with Ang-(1-7).](http://images.novusbio.com/images2/Angiotensin-II-III-Antibody-(Ang-II-E7-(BGN-KA-4L))-Western-Blot-NB100-62346-img0001.jpg)
![Western Blot: Pepsinogen C/PGC/Progastricsin Antibody [NBP1-91011] - Analysis in control (vector only transfected HEK293T lysate) and PGC over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/images2/Pepsinogen-C-PGC-Progastricsin-Antibody-Western-Blot-NBP1-91011-img0013.jpg)
![Immunohistochemistry-Paraffin: Pepsinogen C/PGC/Progastricsin Antibody [NBP1-91011] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/images2/Pepsinogen-C-PGC-Progastricsin-Antibody-Immunohistochemistry-Paraffin-NBP1-91011-img0019.jpg)
![Western Blot: Pepsinogen C/PGC/Progastricsin Antibody [NBP1-91011] - Analysis in human stomach tissue.](http://images.novusbio.com/images2/Pepsinogen-C-PGC-Progastricsin-Antibody-Western-Blot-NBP1-91011-img0009.jpg)
![Western Blot: DAZL Antibody [NB100-2437] - Rat Testes lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/images2/DAZL-Antibody-Western-Blot-NB100-2437-img0005.jpg)
![Immunohistochemistry: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749] - Pax8-CreERT2 mediated beta-galactosidase expression. beta-galactosidase positivity was exclusively found in the tubular epithelium of the kidney of tamoxifen treated mice, with no staining observed in glomeruli or bloods vessels (a, framed area is shown enlarged in b). Kidneys from vehicle treated mice were negative (c). Co-localisation studies revealed X-gal expression within tubules of all renal tubular compartment (proximal tubules-AQP1, distal tubules-THP and collecting ducts-AQP2). Scale bars, 100um. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0148055), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Aquaporin-1-AQP1-Antibody-(1-A5F6)-Immunohistochemistry-NB600-749-img0002.jpg)
![Immunohistochemistry: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749] - Intraparenchymal delivery of lentiviral vectors preferentially infects epithelial cells of both kidneys and urinary bladder. Confocal images of Strawberry expression in renal sections of ELS infected kidneys 60 days post injection. Sections were stained with the indicated antibodies and merged images with DAPI (blue) are presented. Preferential transduction was observed in proximal (AQP1) and distal (THP) renal tubular epithelial cells and renal fibroblasts (CD73) (white arrowheads). Strawberry was not localized to collecting ducts (AQP2) or podocytes (Nephrin) (white arrows). Scale bars, 50 um. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/doifinder/10.1038/srep11061), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Aquaporin-1-AQP1-Antibody-(1-A5F6)-Immunohistochemistry-NB600-749-img0001.jpg)
![Immunohistochemistry: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749] - Slc22a6-CreERT2 mediated beta-galactosidase expression. beta-galactosidase positivity was exclusively found in the tubular epithelium of the kidney of tamoxifen treated mice (a); kidneys from vehicle treated mice were negative (b). Co-localisation studies revealed specific X-gal expression within proximal tubular epithelium (AQP1) with no expression detected in distal tubules (THP) or collecting ducts (AQP2). Scale bars, 100um. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0148055), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Aquaporin 1-AQP1 Antibody (1-A5F6)-Immunohistochemistry-NB600-749-img0003.jpg)
![Western Blot: Albumin Antibody [NB600-41532] - Analysis using the HRP conjugate of NB600-41532. Detection of Albumin in whole mouse liver extracts. Image courtesy of anonymous customer product review.](http://images.novusbio.com/images2/Albumin-Antibody-Western-Blot-NB600-41532-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Albumin Antibody [NB600-41532] - NLRP12 downregulates multiple JNK activating pathways in hepatocytes. For checking the purity of primary hepatocytes, hepatocytes isolated from healthy WT and Nlrp12-/- mouse livers were immunostained with antibody for hepatocyte-specific marker Albumin and counterstained with DAPI. Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/40396), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Albumin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-41532-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: PIWIL1/HIWI Antibody [NBP1-06062] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/images2/PIWIL1-HIWI-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-06062-img0002.jpg)
![Immunohistochemistry-Paraffin: PIWIL1/HIWI Antibody [NBP1-06062] - Staining of paraffin embedded Human Testis. Antibody at 4 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/images2/PIWIL1-HIWI-Antibody-Immunohistochemistry-Paraffin-NBP1-06062-img0003.jpg)
![Flow Cytometry: PIWIL1/HIWI Antibody [NBP1-06062] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/images2/PIWIL1-HIWI-Antibody-Flow-Cytometry-NBP1-06062-img0004.jpg)
![Western Blot: PIWIL4 Antibody (10G9B11) [NBP2-37398] - Western blot analysis using PIWIL4 mouse mAb against PC-3 (1) and PANC-1 (2) cell lysate.](http://images.novusbio.com/images2/PIWIL4-Antibody-(10G9B11)-Western-Blot-NBP2-37398-img0004.jpg)
![Immunohistochemistry: PIWIL4 Antibody (10G9B11) [NBP2-37398] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PIWIL4 mouse mAb with DAB staining.](http://images.novusbio.com/images2/PIWIL4-Antibody-(10G9B11)-Immunohistochemistry-NBP2-37398-img0007.jpg)
![Flow Cytometry: PIWIL4 Antibody (10G9B11) [NBP2-37398] - Flow cytometric analysis of HeLa cells using PIWIL4 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/images2/PIWIL4-Antibody-(10G9B11)-Flow-Cytometry-NBP2-37398-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-232] - Chromosome spread for sycp3. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/images2/SCP3-SYCP3-Antibody-Immunocytochemistry-Immunofluorescence-NB300-232-img0013.jpg)
![Immunohistochemistry-Paraffin: SCP3/SYCP3 Antibody [NB300-232] - IHC analysis of a formalin fixed and paraffin embedded tissue section of Mouse testis using SCP3 antibody at 1:1000 dilution. The signal was detected using HRP-DAB detection method and the nuclei were counterstained with hematoxylin. The antibody generated a specific signal of SCP3 in the spermatogonial cells and the spermatocytes. The signal was strongest in the nuclei of spermatogonial cells.](http://images.novusbio.com/images2/SCP3-SYCP3-Antibody-Immunohistochemistry-Paraffin-NB300-232-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-232] - Exposure to DEHP impairs meiotic progression of oocytes from pachytene to diplotene. Immunolabeling of the oocyte chromosomes with anti-SYCP3 antibody (red) and Hoechst 33342 (blue). All experiments were repeated at least three times independently. (* P<0.05; ** P<0.01) Image collected and cropped by CiteAb from the following publication (http://www.nature.com/doifinder/10.1038/cddis.2017.350), licensed under a CC-BY licence.](http://images.novusbio.com/images2/SCP3-SYCP3 Antibody-Immunocytochemistry-Immunofluorescence-NB300-232-img0016.jpg)
![Western Blot: NANOGP8 Antibody [H00388112-B01P] - Analysis of NANOGP8 expression in transfected 293T cell line by NANOGP8 polyclonal antibody. Lane 1: NANOGP8 transfected lysate(33.55 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/images2/NANOGP8 Antibody-Western Blot-H00388112-B01P-img0002.jpg)
![Western Blot: Renin Antibody (2F10) [NBP2-45552] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Renin.](http://images.novusbio.com/images2/Renin Antibody (2F10)-Western Blot-NBP2-45552-img0010.jpg)
![Immunohistochemistry: Renin Antibody (2F10) [NBP2-45552] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/images2/Renin-Antibody-(2F10)-Immunohistochemistry-NBP2-45552-img0009.jpg)
![Immunohistochemistry: Renin Antibody (2F10) [NBP2-45552] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/images2/Renin-Antibody-(2F10)-Immunohistochemistry-NBP2-45552-img0001.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0006.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.")