PIWIL1/HIWI Antibody

PIWIL1/HIWI was detected in perfusion fixed frozen sections of mouse testes using Goat Anti-Human PIWIL1/HIWI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6548) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Northern­Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm of spermatocytes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

• Reactivity: Hu, Mu
• Applications: IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

PIWIL1/HIWI Antibody Summary

• Immunogen: E. coli-derived recombinant human PIWIL1/HIWI
  Arg82-Thr290
  Accession # Q96J94
• Specificity: Detects human PIWIL1/HIWI in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PIWIL1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PIWIL1/HIWI Antibody

• HIWI
• HIWIpiwi homolog
• MIWI
• piwi (Drosophila)-like 1
• PIWI
• PIWIL1
• piwi-like 1 (Drosophila)
• piwi-like protein 1

Background

PIWI (P-element-induced wimpy testis; also HIWI and PIWIL1) is a 92-98 kDa member of the Piwi subfamily, Argonaute family of proteins. It is expressed in germline cells, particularly spermatocytes and spermatids, and also appears in CD34+ hematopoietic stem cells plus select tumor types. PIWI binds to a series of 28‑34 nucleic acid long noncoding RNAs that participate in gametogenesis. It does so under the influence of Arg methyltransferases, and in conjunction with Tudor‑containing proteins. Human PIWI/PIWIL1 is 861 amino acids (aa) in length. It contains a methylation target region in the N-terminus, followed by an RNA-binding PAZ domain (aa 277-391) and an RNA-cleaving PIWI domain (aa 555-847). There are two isoform variants. One is widely expressed and shows a three aa substitution for aa 1‑89. The second contains a six aa substitution for aa 824-861. Over aa 82-290, human PIWI (HIWI) shares 95% aa sequence identity with mouse PIWI (MIWI).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PIWIL1/HIWI Antibody (AF6548)(4)

Publications using AF6548

• Atkins, GR;Hvasta-Gloria, RL;Ausavarungnirun, C;Pombar, CR;Hardy, JJ;Sukhwani, M;Barnard, EP;Pollock, N;Malizio, M;Sheng, Y;Brieño-Enríquez, MA;Castro, C;Chu, T;Yatsenko, AN;Orwig, KE; Tex11 Mutant Mouse Models of Human Azoospermia bioRxiv : the preprint server for biology 2026-02-18 [PMID: 41756962] (Immunohistochemistry, Mouse)
• AS Bailey, MT Fuller YTHDC2 serves a distinct late role in spermatocytes during germ cell differentiation bioRxiv : the preprint server for biology, 2023-01-23;0(0):. 1/23/2023 [PMID: 36747642]
• D Meseure, S Vacher, S Boudjemaa, M Laé, A Nicolas, R Leclere, W Chemlali, G Champenois, A Schnitzler, L Lesage, T Dubois, I Bieche Biopathological Significance of PIWI-piRNA Pathway Deregulation in Invasive Breast Carcinomas Cancers (Basel), 2020-09-30;12(10):. 2020-09-30 [PMID: 33008024] (IHC, Human)
• Shetty G, Mitchell JM, Lam TNA et al. Donor spermatogenesis in de novo formed seminiferous tubules from transplanted testicular cells in rhesus monkey testis. Hum. Reprod. 10/24/2018 [PMID: 30358843]

Bioinformatics

• Gene Symbol: PIWIL1
• Uniprot:
  • Q96J94()