DAZL Antibody

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Western Blot: DAZL Antibody [NB100-2437] - Rat Testes lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, BvSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

DAZL Antibody Summary

Immunogen
Peptide with sequence C-GNGPQKKSVDR corresponding to internal region according to NP_001342.2.
Predicted Species
Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DAZL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 1 ug/mL
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:32000
Application Notes
Western blot: Approx. 38 kDa band observed in Rat Testis lysates (calculated MW of 31.4 kDa according to NP_001102884.1). Use in ICC/IF reported in scientific literature (PMID: 26916381). Use in IHC reported in scientific literature (PMID: 30302273). Use in IHC-P reported in scientific literature (PMID: 29721205).
Control
Human Ovary Whole Tissue Lysate (Adult Whole Normal)
DAZL Overexpression Lysate
Publications
Read Publications using
NB100-2437 in the following applications:

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 23690628). Human reactivity reported in scientific literature (PMID: 29713018). Mouse reactivity scientific literature (PMID: 29721205). Predicted cross-reactivity based on sequence identity: Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DAZL Antibody

  • DAZHSPGY-like-autosomal
  • DAZL1DAZ-like autosomal
  • DAZLA
  • Deleted in azoospermia-like 1
  • deleted in azoospermia-like
  • germline specific RNA binding protein
  • MGC26406
  • spermatogenesis gene on the Y-like autosomal
  • SPGYLADAZ homolog

Background

Gene ID(s): 1618 (human)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DAZL Antibody (NB100-2437)(11)

We have publications tested in 3 confirmed species: Human, Mouse, Canine.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-P, WB.


Filter By Application
ICC/IF
(2)
IHC
(2)
IHC-P
(4)
WB
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(6)
Canine
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB100-2437 Applications Species
Liu C, Ma Z, Cai Z et Al. Identification of primordial germ cell-like cells as liver metastasis initiating cells in mouse tumour models Cell Discov Mar 24 2020 [PMID: 32218989] (IHC, Mouse) IHC Mouse
Liu C, Cai Z, Jin G et al. Abnormal gametogenesis induced by p53 deficiency promotes tumor progression and drug resistance. Cell Discov. Oct 2 2018 [PMID: 30302273] (IHC, Mouse) IHC Mouse
Levi M, Stemmer SM, Stein J et al. Treosulfan induces distinctive gonadal toxicity compared with busulfan Oncotarget Apr 10 2018 [PMID: 29721205] (IHC-P, Mouse) IHC-P Mouse
Fang F, Angulo B, Xia N et al. A PAX5-OCT4-PRDM1 developmental switch specifies human primordial germ cells Nat. Cell Biol. Jun 1 2018 [PMID: 29713018] (Human) Human
Guo K, Li CH, Wang XY et al. Germ stem cells are active in postnatal mouse ovary under physiological conditions. Mol. Hum. Reprod. May 1 2016 [PMID: 26916381] (ICC/IF, Mouse) ICC/IF Mouse
Levi M, Tzabari M, Savion N et al. Dexrazoxane exacerbates doxorubicin-induced testicular toxicity. Reproduction 2015 Oct 01 [PMID: 26329125] (IHC-P, Mouse) IHC-P Mouse
Dominguez AA, Chiang HR, Sukhwani M et al. Human germ cell formation in xenotransplants of induced pluripotent stem cells carrying X chromosome aneuploidies. Sci Rep. 2014 Sep 22 [PMID: 25242416] (IHC-P, Mouse) IHC-P Mouse
Harkey MA, Asano A, Zoulas ME et al. Isolation, genetic manipulation, and transplantation of canine spermatogonial stem cells: progress toward transgenesis through the male germ-line. Reproduction. 2013 Jun 14 [PMID: 23690628] (WB, ICC/IF, IHC-P, Canine) WB, ICC/IF, IHC-P Canine
He Z, Jiang J, Kokkinaki M, Golestaneh N et al. Gdnf Upregulates c-Fos Transcription via the Ras/Erk1/2 Pathway to Promote Mouse Spermatogonial Stem Cell Proliferation. Stem Cells, 2008;26(1):266-78. 2008 January 1. [PMID: 17962702]
Hermann, B P, M Sukhwani et al. Characterization, Cryopreservation, Ablation of Spermatogonial Stem Cells in Adult Rhesus Macaques. 25: 2330-2338. 2007 [PMID: 17585169]
Show All 11 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DAZL Antibody (NB100-2437) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DAZL
Entrez
Uniprot