DAZL Antibody

Western Blot: DAZL Antibody [NB100-2437] - Rat Testes lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Ca, Bv
• Applications: WB, ELISA, ICC/IF, IHC, Func
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

DAZL Antibody Summary

• Immunogen: Peptide with sequence C-GNGPQKKSVDR corresponding to internal region according to NP_001342.2.
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DAZL
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • PCR
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.5 - 1 ug/mL
• Application Notes: Use in PCR reported in scientific literature (PMID:33791879) Western blot: Approx. 38 kDa band observed in Rat Testis lysates (calculated MW of 31.4 kDa according to NP_001102884.1). Use in ICC/IF reported in scientific literature (PMID: 26916381). Use in IHC reported in scientific literature (PMID: 30302273). Use in IHC-P reported in scientific literature (PMID: 29721205).
• Control:

  Human Ovary Whole Tissue Lysate (Adult Whole Normal)
  DAZL Overexpression Lysate

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 23690628). Human reactivity reported in scientific literature (PMID: 29713018). Mouse reactivity scientific literature (PMID: 29721205) . Predicted cross-reactivity based on sequence identity: Bovine.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DAZL Antibody

• DAZHSPGY-like-autosomal
• DAZL1DAZ-like autosomal
• DAZLA
• Deleted in azoospermia-like 1
• deleted in azoospermia-like
• germline specific RNA binding protein
• MGC26406
• spermatogenesis gene on the Y-like autosomal
• SPGYLADAZ homolog

Background

Gene ID(s): 1618 (human)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DAZL Antibody (NB100-2437)(15)

Publications using NB100-2437

• Zhan Ma, Fengyu Z, Aiping L et al. Somatic-to-primordial Germ Cell-Like Transformation is Critical in Tumour Initiation of Mouse Breast Tumour 4T1 Cells Preprint 2022-11-04 [PMID: 37168326] (KO, WB, Mouse)
• Sun S, Jiang Y, Zhang Q et al. Znhit1 controls meiotic initiation in male germ cells by coordinating with Stra8 to activate meiotic gene expression Developmental cell 2022-04-11 [PMID: 35413238]
• Sayed WM, Elzainy A Impact of platelet-rich plasma versus selenium in ameliorating induced toxicity in rat testis: histological, immunohistochemical, and molecular study Cell and tissue research 2021-04-01 [PMID: 33791879] (PCR)
• Liu, C, Ma, Z Et al. Identification of primordial germ cell-like cells as liver metastasis initiating cells in mouse tumour models. Cell Discov 2020-03-24 [PMID: 33758163]
• Liu C, Ma Z, Cai Z et Al. Identification of primordial germ cell-like cells as liver metastasis initiating cells in mouse tumour models Cell Discov 2020-03-24 [PMID: 32218989] (IF/IHC, Mouse)
• Liu C, Cai Z, Jin G et al. Abnormal gametogenesis induced by p53 deficiency promotes tumor progression and drug resistance. Cell Discov. 2018-10-02 [PMID: 30302273] (IF/IHC, Mouse)
• Levi M, Stemmer SM, Stein J et al. Treosulfan induces distinctive gonadal toxicity compared with busulfan Oncotarget 2018-04-10 [PMID: 29721205] (IHC-P, Mouse)
• Fang F, Angulo B, Xia N et al. A PAX5-OCT4-PRDM1 developmental switch specifies human primordial germ cells Nat. Cell Biol. 2018-06-01 [PMID: 29713018] (Human)
• Guo K, Li CH, Wang XY et al. Germ stem cells are active in postnatal mouse ovary under physiological conditions. Mol. Hum. Reprod. 2016-05-01 [PMID: 26916381] (ICC/IF, Mouse)
• Levi M, Tzabari M, Savion N et al. Dexrazoxane exacerbates doxorubicin-induced testicular toxicity. Reproduction 2015-10-01 [PMID: 26329125] (IHC-P, Mouse)
• Dominguez AA, Chiang HR, Sukhwani M et al. Human germ cell formation in xenotransplants of induced pluripotent stem cells carrying X chromosome aneuploidies. Sci Rep. 2014-09-22 [PMID: 25242416] (IHC-P, Mouse)
• Harkey MA, Asano A, Zoulas ME et al. Isolation, genetic manipulation, and transplantation of canine spermatogonial stem cells: progress toward transgenesis through the male germ-line. Reproduction. 2013-06-14 [PMID: 23690628] (ICC/IF, IHC-P, WB, Canine)
• He Z, Jiang J, Kokkinaki M et al. Gdnf upregulates c-Fos transcription via the Ras/Erk1/2 pathway to promote mouse spermatogonial stem cell proliferation. Stem Cells. 2008-01-01 [PMID: 17962702]
• Hermann, B P, M Sukhwani et al. Characterization, Cryopreservation, Ablation of Spermatogonial Stem Cells in Adult Rhesus Macaques. 25: 2330-2338. 2007-01-01 [PMID: 17585169]
• Cauffman G, Van de Velde H, Liebaers I, Van Steirteghem A. DAZL expression in human oocytes, preimplantation embryos and embryonic stem cells. Mol Hum Reprod 2005-06-01 [PMID: 15879466]

Bioinformatics

• Gene Symbol: DAZL
• Entrez:
  • 1618(Human)
• Uniprot:
  • Q92904(Human)