Aquaporin 1/AQP1 Antibody (1/A5F6)

• Reactivity: Hu, Mu, Rt, Rb, Ze
• Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
• Clone: 1/A5F6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Aquaporin 1/AQP1 Antibody (1/A5F6) Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 249 - 269 of Rat Aquaporin 1.
• Localization: Plasma membrane
• Specificity: Antibody recognises an epitope within the cytoplasmic domain of the water-specific channel aquaporin 1.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: AQP1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000-1:5000
  • ELISA 1:1000-1:20000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:500-1:1000
  • Immunohistochemistry-Paraffin 1:500-1:1000
• Application Notes: ELISA: Use at a dilution of 1/1000. IHC-P: Use at a dilution of 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.IHC-Fr: Use at a dilution of 1/500. IP: Use at a dilution of 1/1000. WB: Use at a dilution of 1/1000. Predicted molecular weight: 32.3 kDa. Immunofluorescence staining reported successful by customer review.
• Control:

  Aquaporin 1/AQP1 Overexpression Lysate
  Human Kidney Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Phosphate buffered saline, 0.1% Bovine Serum Albumin
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Aquaporin 1/AQP1 Antibody (1/A5F6)

• 28-kDa
• AQP1
• AQP-1
• AQP-CHIP
• aquaporin 1 (channel-forming integral protein, 28kDa)
• aquaporin 1 (Colton blood group)
• Aquaporin 1
• Aquaporin-CHIP
• CHIP28
• CHIP2828kDa, CO blood group)
• CO
• MGC26324

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Aquaporin 1/AQP1 Antibody (NB600-749)(6)

Publications using NB600-749

• Espana-Agusti J, Zou X, Wong K et al. Generation and Characterisation of a Pax8-CreERT2 Transgenic Line and a Slc22a6-CreERT2 Knock-In Line for Inducible and Specific Genetic Manipulation of Renal Tubular Epithelial Cells. PLoS One 2016 Feb 11 [PMID: 26866916]
• Espana-Agusti J, Tuveson DA, Adams DJ, Matakidou A. A minimally invasive, lentiviral based method for the rapid and sustained genetic manipulation of renal tubules. Sci Rep 2015 [PMID: 26046460] (IHC, Mouse)
• Francone, VP et al. Signaling from the secretory granule to the nucleus: Uhmk1 and PAM. 2010 [PMID: 20573687]
• Felszeghy, S et al. Expression of aquaporin isoforms during human and mouse tooth development. 2004 [PMID: 15003543]
• Nagy, G et al. Development and characterisation of a monoclonal antibody family against aquaporin 1 (AQP1) and aquaporin 4 (AQP4). 2002 [PMID: 12172575]
• Bodis, B et al. Active water selective channels in the stomach: investigation of aquaporins after ethanol and capsaicin treatment in rats. 2001 [PMID: 11595449]

Review for Aquaporin 1/AQP1 Antibody (NB600-749) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Anonymous IF Rat 11/30/2010

Summary

• Application: Immunofluorescence
• Sample Tested: Rat Lung Tissue
• Species: Rat
• Lot: 130209
• Comments: Used at 1:500 dilution

Blocking

• Blocking Details: Blocking Buffer: PBS with 5% FBS, Blocking Time: 30 minutes, Blocking Temp: room temperature

Primary Anitbody

• Dilution Ratio: Primary Ab Incubation Dilution Buffer: PBS with 5% FBS, Dilution Ratio: 1:500, Incubation Time: 1 hr, Incubation Temp:RT

Secondary Antibody

• Secondary Description: Secondary Ab: Millipore anti-mouse IgG Cy3, Secondary Ab Dilution: 1:400
• Secondary Manufacturer Cat#: AP181C

Details

• Detection Notes: Detection Visualization Method: Fluorescence at 570nm
• Fixation Details: Sample Preparation: OCT

Comments

• Comments: Used at 1:500 dilution

FAQs for Aquaporin 1/AQP1 Antibody (NB600-749). (Showing 1 - 1 of 1 FAQs).

1. Are any of your Aquaporin-1 antibodies suitable for live cell staining? I am looking at doing flow cytometry on live cells and further isolation of the positive cells to purify the population.
   • A list of our Aquaporin-1 antibodies suitable for use in flow cytometry can be found using this link. Of note, NB600-741 is known to bind to an extracellular domain, so should be suitable for your use.

Bioinformatics

• Gene Symbol: AQP1
• Entrez:
  • 358(Human)
  • 11826(Mouse)
  • 25240(Rat)
• Uniprot:
  • P29975 (Rat)