Aquaporin 1/AQP1 Antibody (1/A5F6)

Images

 
Immunohistochemistry: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749] - Pax8-CreERT2 mediated beta-galactosidase expression. beta-galactosidase positivity was exclusively found in the tubular epithelium of the kidney of ...read more
Immunohistochemistry: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749] - Intraparenchymal delivery of lentiviral vectors preferentially infects epithelial cells of both kidneys and urinary bladder. Confocal images of ...read more
Immunohistochemistry: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749] - Slc22a6-CreERT2 mediated beta-galactosidase expression. beta-galactosidase positivity was exclusively found in the tubular epithelium of the kidney ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Rb, ZeSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
1/A5F6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Aquaporin 1/AQP1 Antibody (1/A5F6) Summary

Immunogen
Synthetic peptide, corresponding to amino acids 249 - 269 of Rat Aquaporin 1.
Localization
Plasma membrane
Specificity
Antibody recognises an epitope within the cytoplasmic domain of the water-specific channel aquaporin 1.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
AQP1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:1000-1:20000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:500-1:1000
  • Immunohistochemistry-Paraffin 1:500-1:1000
  • Western Blot 1:1000-1:5000
Application Notes
ELISA: Use at a dilution of 1/1000. IHC-P: Use at a dilution of 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.IHC-Fr: Use at a dilution of 1/500. IP: Use at a dilution of 1/1000. WB: Use at a dilution of 1/1000. Predicted molecular weight: 32.3 kDa. Immunofluorescence staining reported successful by customer review.
Control
Aquaporin 1/AQP1 Overexpression Lysate
Human Kidney Whole Tissue Lysate (Adult Whole Normal)
Reviewed Applications
Read 1 Review rated 4
using
NB600-749 in the following applications:

Publications
Read Publications using
NB600-749 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 0.1% BSA
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Aquaporin 1/AQP1 Antibody (1/A5F6)

  • 28-kDa
  • AQP1
  • AQP-1
  • AQP-CHIP
  • aquaporin 1 (channel-forming integral protein, 28kDa)
  • aquaporin 1 (Colton blood group)
  • Aquaporin 1
  • Aquaporin-CHIP
  • CHIP28
  • CHIP2828kDa, CO blood group)
  • CO
  • MGC26324

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Aquaporin 1/AQP1 Antibody (NB600-749)(12)

We have publications tested in 3 confirmed species: Human, Mouse, Rabbit.

We have publications tested in 2 applications: ICC/IF, IHC.


Filter By Application
ICC/IF
(1)
IHC
(6)
All Applications
Filter By Species
Human
(2)
Mouse
(4)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NB600-749 Applications Species
Rossbach B, Hariharan K, Mah N et al. Human iPSC-Derived Renal Cells Change Their Immunogenic Properties during Maturation: Implications for Regenerative Therapies Cells Apr 13 2022 [PMID: 35456007] (ICC/IF, Human) ICC/IF Human
Lim S, Kim YJ, Park S Et al. mTORC1-induced retinal progenitor cell overproliferation leads to accelerated mitotic aging and degeneration of descendent MUller glia eLife Oct 22 2021 [PMID: 34677125] (IHC, Mouse) IHC Mouse
Purohit D, Finkel DA, Malfa A et al. Human Cord Blood Derived Unrestricted Somatic Stem Cells Restore Aquaporin Channel Expression, Reduce Inflammation and Inhibit the Development of Hydrocephalus After Experimentally Induced Perinatal Intraventricular Hemorrhage Frontiers in cellular neuroscience Apr 9 2021 [PMID: 33897371] (IHC, Rabbit) IHC Rabbit
Zheng C, Huang L et al. Inhibition of STAT3 in tubular epithelial cells prevents kidney fibrosis and nephropathy in STZ-induced diabetic mice. Cell Death Dis Jul 11 2019 [PMID: 31699972] (IHC, Mouse) IHC Mouse
Gong Y, He X et al. SCF/SCFR signaling plays an important role in the early morphogenesis and neurogenesis of human embryonic neural retina. Development Oct 17 2019 [PMID: 31548215] (IHC, Human) IHC Human
Moon K H, Kim H T et al. Differential Expression of NF2 in Neuroepithelial Compartments Is Necessary for Mammalian Eye Development. Dev Cell Aug 1 2018 [PMID: 29249622] (IHC, Mouse) IHC Mouse
Espana-Agusti J, Zou X, Wong K et al. Generation and Characterisation of a Pax8-CreERT2 Transgenic Line and a Slc22a6-CreERT2 Knock-In Line for Inducible and Specific Genetic Manipulation of Renal Tubular Epithelial Cells. PLoS One 2016 Feb 11 [PMID: 26866916]
Espana-Agusti J, Tuveson DA, Adams DJ, Matakidou A. A minimally invasive, lentiviral based method for the rapid and sustained genetic manipulation of renal tubules. Sci Rep 2015 [PMID: 26046460] (IHC, Mouse) IHC Mouse
Francone, VP et al. Signaling from the secretory granule to the nucleus: Uhmk1 and PAM. 2010 [PMID: 20573687]
Felszeghy, S et al. Expression of aquaporin isoforms during human and mouse tooth development. 2004 [PMID: 15003543]
Show All 12 Publications.

Review for Aquaporin 1/AQP1 Antibody (NB600-749) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NB600-749:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence Aquaporin 1/AQP1 NB600-749
Enlarge
4
reviewed by:
Keri Newell
IF Rat 11/30/2010
View

Summary

ApplicationImmunofluorescence
Sample TestedRat Lung Tissue
SpeciesRat
Lot130209

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Aquaporin 1/AQP1 Antibody (NB600-749). (Showing 1 - 1 of 1 FAQs).

  1. Are any of your Aquaporin-1 antibodies suitable for live cell staining? I am looking at doing flow cytometry on live cells and further isolation of the positive cells to purify the population.
    • A list of our Aquaporin-1 antibodies suitable for use in flow cytometry can be found using this link. Of note, NB600-741 is known to bind to an extracellular domain, so should be suitable for your use.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Aquaporin 1/AQP1 Antibody (NB600-749)

Discover related pathways, diseases and genes to Aquaporin 1/AQP1 Antibody (NB600-749). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Recent Reviews

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4
1
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1
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Keri Newell
11/30/2010
Application: IF
Species: Rat

Bioinformatics

Gene Symbol AQP1
Entrez
Uniprot