Aquaporin 1/AQP1 Antibody (1/A5F6)

Immunohistochemistry: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749] - Pax8-CreERT2 mediated beta-galactosidase expression. beta-galactosidase positivity was exclusively found in the tubular epithelium of the kidney of tamoxifen treated mice, with no staining observed in glomeruli or bloods vessels (a, framed area is shown enlarged in b). Kidneys from vehicle treated mice were negative (c). Co-localisation studies revealed X-gal expression within tubules of all renal tubular compartment (proximal tubules-AQP1, distal tubules-THP and collecting ducts-AQP2). Scale bars, 100um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0148055), licensed under a CC-BY licence.

Immunohistochemistry: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749] - Intraparenchymal delivery of lentiviral vectors preferentially infects epithelial cells of both kidneys and urinary bladder. Confocal images of Strawberry expression in renal sections of ELS infected kidneys 60 days post injection. Sections were stained with the indicated antibodies and merged images with DAPI (blue) are presented. Preferential transduction was observed in proximal (AQP1) and distal (THP) renal tubular epithelial cells and renal fibroblasts (CD73) (white arrowheads). Strawberry was not localized to collecting ducts (AQP2) or podocytes (Nephrin) (white arrows). Scale bars, 50 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/doifinder/10.1038/srep11061), licensed under a CC-BY licence.

Immunohistochemistry: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749] - Slc22a6-CreERT2 mediated beta-galactosidase expression. beta-galactosidase positivity was exclusively found in the tubular epithelium of the kidney of tamoxifen treated mice (a); kidneys from vehicle treated mice were negative (b). Co-localisation studies revealed specific X-gal expression within proximal tubular epithelium (AQP1) with no expression detected in distal tubules (THP) or collecting ducts (AQP2). Scale bars, 100um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0148055), licensed under a CC-BY licence.

• Reactivity: Hu, Mu, Rt, Rb, Ze
• Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
• Clone: 1/A5F6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Aquaporin 1/AQP1 Antibody (1/A5F6) Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 249 - 269 of Rat Aquaporin 1.
• Localization: Plasma membrane
• Specificity: Antibody recognises an epitope within the cytoplasmic domain of the water-specific channel aquaporin 1.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: AQP1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000-1:5000
  • ELISA 1:1000-1:20000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:500-1:1000
  • Immunohistochemistry-Paraffin 1:500-1:1000
• Application Notes: ELISA: Use at a dilution of 1/1000. IHC-P: Use at a dilution of 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.IHC-Fr: Use at a dilution of 1/500. IP: Use at a dilution of 1/1000. WB: Use at a dilution of 1/1000. Predicted molecular weight: 32.3 kDa. Immunofluorescence staining reported successful by customer review.
• Control:

  Aquaporin 1/AQP1 Overexpression Lysate
  Human Kidney Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Phosphate buffered saline, 0.1% Bovine Serum Albumin
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Aquaporin 1/AQP1 Antibody (1/A5F6)

• 28-kDa
• AQP1
• AQP-1
• AQP-CHIP
• aquaporin 1 (channel-forming integral protein, 28kDa)
• aquaporin 1 (Colton blood group)
• Aquaporin 1
• Aquaporin-CHIP
• CHIP28
• CHIP2828kDa, CO blood group)
• CO
• MGC26324

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Aquaporin 1/AQP1 Antibody (NB600-749)(9)

Publications using NB600-749

• Zheng C, Huang L et al. Inhibition of STAT3 in tubular epithelial cells prevents kidney fibrosis and nephropathy in STZ-induced diabetic mice. Cell Death Dis Jul 11 2019 [PMID: 31699972] (IHC, Mouse)
• Gong Y, He X et al. SCF/SCFR signaling plays an important role in the early morphogenesis and neurogenesis of human embryonic neural retina. Development Oct 17 2019 [PMID: 31548215] (IHC, Human)
• Moon K H, Kim H T et al. Differential Expression of NF2 in Neuroepithelial Compartments Is Necessary for Mammalian Eye Development. Dev Cell Aug 1 2018 [PMID: 29249622] (IHC, Mouse)
• Espana-Agusti J, Zou X, Wong K et al. Generation and Characterisation of a Pax8-CreERT2 Transgenic Line and a Slc22a6-CreERT2 Knock-In Line for Inducible and Specific Genetic Manipulation of Renal Tubular Epithelial Cells. PLoS One 2016 Feb 11 [PMID: 26866916]
• Espana-Agusti J, Tuveson DA, Adams DJ, Matakidou A. A minimally invasive, lentiviral based method for the rapid and sustained genetic manipulation of renal tubules. Sci Rep 2015 [PMID: 26046460] (IHC, Mouse)
• Francone, VP et al. Signaling from the secretory granule to the nucleus: Uhmk1 and PAM. 2010 [PMID: 20573687]
• Felszeghy, S et al. Expression of aquaporin isoforms during human and mouse tooth development. 2004 [PMID: 15003543]
• Nagy, G et al. Development and characterisation of a monoclonal antibody family against aquaporin 1 (AQP1) and aquaporin 4 (AQP4). 2002 [PMID: 12172575]
• Bodis, B et al. Active water selective channels in the stomach: investigation of aquaporins after ethanol and capsaicin treatment in rats. 2001 [PMID: 11595449]

Review for Aquaporin 1/AQP1 Antibody (NB600-749) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Anonymous IF Rat 11/30/2010

Summary

• Application: Immunofluorescence
• Sample Tested: Rat Lung Tissue
• Species: Rat
• Lot: 130209
• Comments: Used at 1:500 dilution

Blocking

• Blocking Details: Blocking Buffer: PBS with 5% FBS, Blocking Time: 30 minutes, Blocking Temp: room temperature

Primary Anitbody

• Dilution Ratio: Primary Ab Incubation Dilution Buffer: PBS with 5% FBS, Dilution Ratio: 1:500, Incubation Time: 1 hr, Incubation Temp:RT

Secondary Antibody

• Secondary Description: Secondary Ab: Millipore anti-mouse IgG Cy3, Secondary Ab Dilution: 1:400
• Secondary Manufacturer Cat#: AP181C

Details

• Detection Notes: Detection Visualization Method: Fluorescence at 570nm
• Fixation Details: Sample Preparation: OCT

Comments

• Comments: Used at 1:500 dilution

FAQs for Aquaporin 1/AQP1 Antibody (NB600-749). (Showing 1 - 1 of 1 FAQs).

1. Are any of your Aquaporin-1 antibodies suitable for live cell staining? I am looking at doing flow cytometry on live cells and further isolation of the positive cells to purify the population.
   • A list of our Aquaporin-1 antibodies suitable for use in flow cytometry can be found using this link. Of note, NB600-741 is known to bind to an extracellular domain, so should be suitable for your use.

Bioinformatics

• Gene Symbol: AQP1
• Entrez:
  • 358(Human)
  • 11826(Mouse)
  • 25240(Rat)
• Uniprot:
  • P29975 (Rat)