Aquaporin 1/AQP1 Antibody (1/A5F6)

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Product Details

Summary
Reactivity Hu, Mu, Rt, Rb, ZeSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
1/A5F6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Aquaporin 1/AQP1 Antibody (1/A5F6) Summary

Immunogen
Synthetic peptide, corresponding to amino acids 249 - 269 of Rat Aquaporin 1.
Localization
Plasma membrane
Specificity
Antibody recognises an epitope within the cytoplasmic domain of the water-specific channel aquaporin 1.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
AQP1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • ELISA 1:1000-1:20000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:500-1:1000
  • Immunohistochemistry-Paraffin 1:500-1:1000
Application Notes
ELISA: Use at a dilution of 1/1000. IHC-P: Use at a dilution of 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.IHC-Fr: Use at a dilution of 1/500. IP: Use at a dilution of 1/1000. WB: Use at a dilution of 1/1000. Predicted molecular weight: 32.3 kDa. Immunofluorescence staining reported successful by customer review.
Control
Aquaporin 1/AQP1 Overexpression Lysate
Human Kidney Whole Tissue Lysate (Adult Whole Normal)
Reviewed Applications
Read 1 Review rated 4
using
NB600-749 in the following applications:

Publications
Read Publications using
NB600-749 in the following applications:

  • IHC
    1 publication

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Phosphate buffered saline, 0.1% Bovine Serum Albumin
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Aquaporin 1/AQP1 Antibody (1/A5F6)

  • 28-kDa
  • AQP1
  • AQP-1
  • AQP-CHIP
  • aquaporin 1 (channel-forming integral protein, 28kDa)
  • aquaporin 1 (Colton blood group)
  • Aquaporin 1
  • Aquaporin-CHIP
  • CHIP28
  • CHIP2828kDa, CO blood group)
  • CO
  • MGC26324

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB600-749
Species: Hu, Mu, Rt, Rb, Ze
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for Aquaporin 1/AQP1 Antibody (NB600-749)(6)

Review for Aquaporin 1/AQP1 Antibody (NB600-749) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NB600-749:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence Aquaporin 1/AQP1 NB600-749
Enlarge
reviewed by:
Anonymous
IF Rat 11/30/2010
View

Summary

ApplicationImmunofluorescence
Sample TestedRat Lung Tissue
SpeciesRat
Lot130209
CommentsUsed at 1:500 dilution

Blocking

Blocking DetailsBlocking Buffer: PBS with 5% FBS, Blocking Time: 30 minutes, Blocking Temp: room temperature

Primary Anitbody

Dilution RatioPrimary Ab Incubation Dilution Buffer: PBS with 5% FBS, Dilution Ratio: 1:500, Incubation Time: 1 hr, Incubation Temp:RT

Secondary Antibody

Secondary DescriptionSecondary Ab: Millipore anti-mouse IgG Cy3, Secondary Ab Dilution: 1:400
Secondary Manufacturer Cat#AP181C

Details

Detection NotesDetection Visualization Method: Fluorescence at 570nm
Fixation DetailsSample Preparation: OCT

Comments

CommentsUsed at 1:500 dilution

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Aquaporin 1/AQP1 Antibody (NB600-749). (Showing 1 - 1 of 1 FAQs).

  1. Are any of your Aquaporin-1 antibodies suitable for live cell staining? I am looking at doing flow cytometry on live cells and further isolation of the positive cells to purify the population.
    • A list of our Aquaporin-1 antibodies suitable for use in flow cytometry can be found using this link. Of note, NB600-741 is known to bind to an extracellular domain, so should be suitable for your use.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Blogs on Aquaporin 1/AQP1

There are no specific blogs for Aquaporin 1/AQP1, but you can read our latest blog posts.
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Recent Reviews

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5
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1
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Anonymous
11/30/2010
Application: IF
Species: Rat

Bioinformatics

Gene Symbol AQP1
Entrez
Uniprot