VASA Antibody - BSA Free

Immunohistochemistry-Paraffin: VASA Antibody [NBP2-24558] - Analysis of DDX4 in mouse testis tissue using an isotype control (top left) and this antibody (bottom left, right) at 5 ug/ml.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Eq, Pm, Xp, Ze
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

VASA Antibody - BSA Free Summary

• Immunogen: A portion of amino acids 700-724 of DDX4 was used as the immunogen.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DDX4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence reported in scientific literature (PMID 34183774)
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml

Reactivity Notes

Orangutan (100%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for VASA Antibody - BSA Free

• DDX4
• DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
• DEAD box protein 4
• EC 3.6.1
• EC 3.6.4.13
• MGC111074
• probable ATP-dependent RNA helicase DDX4
• Vasa homolog
• VASA
• VASADEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4

Background

VASA is implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. VASA is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VASA Antibody (NBP2-24558)(7)

Publications using NBP2-24558

• Nagham Ali, Eitan Lunenfeld, Mahmoud Huleihel, Jaime Catalan, Manuel Álvarez-Rodríguez Effect of IL-1 beta on the Development of Spermatogenesis In Vitro in Normal and Busulfan-Treated Immature Mice International Journal of Molecular Sciences 2024-04-30 [PMID: 38732137]
• Jorban, A;Lunenfeld, E;Huleihel, M; Effect of Temperature on the Development of Stages of Spermatogenesis and the Functionality of Sertoli Cells In Vitro International journal of molecular sciences 2024-02-10 [PMID: 38396838]
• AbuMadighem A, Shuchat S, Lunenfeld E et al. Testis on a chip - a microfluidic 3-dimensional culture system for the development of spermatogenesisin-vitro Biofabrication 2022-03-25 [PMID: 35334473]
• Rore H, Owen N, Pina-Aguilar Re Et Al. Testicular somatic cell-like cells derived from embryonic stem cells induce differentiation of epiblasts into germ cells Communications biology 2021-06-28 [PMID: 34183774] (ICC/IF)
• Sawaied A, Arazi E, AbuElhija A et al. The Presence of Colony-Stimulating Factor-1 and Its Receptor in Different Cells of the Testis; It Involved in the Development of Spermatogenesis In Vitro International Journal of Molecular Sciences 2021-02-26 [PMID: 33652607]
• Bagdadi N, Sawaied A, AbuMadighem A, et al. The Expression Levels and Cellular Localization of Pigment Epithelium Derived Factor (PEDF) in Mouse Testis: Its Possible Involvement in the Differentiation of Spermatogonial Cells International journal of molecular sciences 2021-01-24 [PMID: 33498962] (IHC-P, Mouse)
• Sawaied A, Lunenfeld E, Huleihel M. Interleukin-34, a Novel Paracrine/Autocrine Factor in Mouse Testis, and Its Possible Role in the Development of Spermatogonial Cells In Vitro International Journal of Molecular Sciences 2020-10-30 [PMID: 33143373]

Bioinformatics

• Gene Symbol: DDX4
• Uniprot:
  • AF262962.1(Human)