USP20 Antibody - BSA Free

Western Blot: USP20 Antibody [NB100-59837] - Detection of Mouse USP20/VDU2 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-USP20/VDU2 antibody NB100-59837 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Western Blot: USP20 Antibody [NB100-59837] - Detection of Human USP20/VDU2 on HeLa whole cell lysate (5, 15 and 50 microgram for WB; 1mg for IP, 20% of IP loaded) and 293T (T; 50 microgram) using NB100-59837. USP20/VDU2 was also immunoprecipitated by another rabbit anti-USP20/VDU2 antibody.

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

USP20 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit USP20 Antibody - BSA Free (NB100-59837) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 863 and 913 of human ubiquitin specific peptidase 20 (pVHL-interacting deubiquitinating enzyme 2) using the numbering given in entry NP_006667.2 (GeneID 10868).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: USP20
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:10-1:500
  • Western Blot
• Control:

  USP20 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for USP20 Antibody - BSA Free

• Deubiquitinating enzyme 20
• EC 3.4.19.12
• KIAA1003hVDU2
• LSFR3A
• pVHL-interacting deubiquitinating enzyme 2
• ubiquitin carboxyl-terminal hydrolase 20
• ubiquitin specific peptidase 20
• ubiquitin specific protease 20
• ubiquitin thioesterase 20
• Ubiquitin Thiolesterase 20
• Ubiquitin-specific-processing protease 20
• USP20
• VDU2
• VHL-interacting deubiquitinating enzyme 2

Background

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: USP20
• Entrez:
  • 10868(Human)
• Uniprot:
  • NP_006667.2(Human)