VAC14 Antibody - BSA Free

Western Blot: VAC14 Antibody [NBP2-44278] - Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. NBP2-44278 was used for IP at 6 ug per reaction. VAC14 was also Immunoprecipitated by another rabbit anti-VAC14 antibody.

Western Blot: VAC14 Antibody [NBP2-44278] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer.

• Reactivity: Hu, Rt, Bv
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

VAC14 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit VAC14 Antibody - BSA Free (NBP2-44278) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The epitope recognized by this antibody maps to a region between residue 732 to 782 of human Protein VAC14 homolog using the numbering given in entry Q08AM6.1 (GeneID 55697).
• Predicted Species: Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: VAC14
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Western Blot 1:2000 - 1:10000
• Application Notes: Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for VAC14 Antibody - BSA Free

• ArPIKfyve
• FLJ10305
• FLJ36622
• MGC149816
• protein VAC14 homolog
• Tax1 (human T-cell leukemia virus type I) binding protein 1
• Tax1 (human T-cell leukemia virus type I) binding protein 2
• Tax1-binding protein 2
• TAX1BP2FLJ46582
• TRXMGC149815
• Vac14 homolog (S. cerevisiae)

Background

Phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2) is a low-abundance signaling molecule. A regulatory complex made up of VAC14 and FIG4 (MIM 609390) control synthesis of PI(3,5)P2 by activating PI(3)P kinase, FAB1 (PIP5K3; MIM 609414). The VAC14/FIG4 complex also functions in the breakdown of PI(3,5)P2 (Zhang et al., 2007 [PubMed 17956977]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VAC14