MARCH6 Antibody

Immunohistochemistry-Paraffin: MARCH6 Antibody [NBP1-06088] - (3.75ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

MARCH6 Antibody Summary

• Immunogen: Peptide with sequence C-QRLVNYERKSGKQ corresponding to internal region according to NP_005876.2.
• Epitope: C-QRLVNYERKSGKQ
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MARCHF6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:16000
• Application Notes: WB: Preliminary experiments in human kidney, thymus and ovary lysates gave no specific signal but low background.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MARCH6 Antibody

• E3 ubiquitin-protein ligase MARCH6
• EC 6.3.2.-
• MARCH-VIDOA10
• membrane-associated ring finger (C3HC4) 6
• Membrane-associated RING finger protein 6
• Membrane-associated RING-CH protein VI
• Protein TEB-4
• RNF176KIAA0597Doa10 homolog
• TEB4RING finger protein 176

Background

MARCH6 (Membrane associated RING finger protein 6) belongs to the MARCH family, which contains at least seven membrane associated RING-CH (MARCH)proteins. MARCH proteins are E3 ubiquitin ligases and are located to subcellular membranes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MARCH6 Antibody (NBP1-06088)(1)

Publication using NBP1-06088

• Kreft SG, Wang L, Hochstrasser M. Membrane topology of the yeast endoplasmic reticulum-localized ubiquitin ligase Doa10 and comparison with its human ortholog TEB4 (MARCH-VI). J Biol Chem 2006-02-24 [PMID: 16373356]

Bioinformatics

• Gene Symbol: MARCHF6
• Entrez:
  • 10299(Human)
  • 223455(Mouse)
• Uniprot:
  • NP_005876.2(Human)