USP33 Antibody

Images

 
Western Blot: USP33 Antibody [NB110-40693] - Detection of Human USP33 on HeLa whole cell lysate using NB110-40693. USP33 was also immunoprecipitated by rabbit anti-USP33 antibody NB110-40692.
Immunohistochemistry: USP33 Antibody [NB110-40693] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- USP33 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: USP33 Antibody [NB110-40693] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-USP33 used at a dilution of 1:250.
Immunohistochemistry-Paraffin: USP33 Antibody [NB110-40693] - FFPE section of mouse renal cell carcinoma. Affinity purified rabbit anti-USP33 used at a dilution of 1:250.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

USP33 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 900 and the C-terminus (residue 942) of human Ubiquitin Specific Peptidase 33 using the numbering given in entry NP_055832.3 (GeneID 23032).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
USP33
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry 1:250
  • Immunohistochemistry-Paraffin 1:250
  • Immunoprecipitation 2-5 ug/mg lysate
Publications
Read Publications using NB110-40693.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for USP33 Antibody

  • Deubiquitinating enzyme 33
  • EC 3.1.2.15
  • EC 3.4.19.12
  • hVDU1
  • KIAA1097MGC16868
  • pVHL-interacting deubiquitinating enzyme 1
  • ubiquitin carboxyl-terminal hydrolase 33
  • ubiquitin specific peptidase 33
  • ubiquitin thioesterase 33
  • Ubiquitin thiolesterase 33
  • Ubiquitin-specific-processing protease 33
  • VDU1ubiquitin specific protease 33
  • VHL-interacting deubiquitinating enzyme 1

Background

A novel protein named VHL-interacting deubiquitinating enzyme 1 (VDU1/USP33) was identified as being able to directly interact with pVHL in vitro and in vivo. The VDU1-interacting region in pVHL is located in its beta-domain, and several naturally occurring mutations located in this domain disrupt the interaction between pVHL and VDU1 protein. VDU1 is able to be ubiquitinated via a pVHL-dependent pathway for proteasomal degradation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for USP33 Antibody (NB110-40693)(3)

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol USP33
Entrez
Uniprot