USP33 Antibody - BSA Free

Western Blot: USP33 Antibody [NB110-40693] - Detection of Human USP33 on HeLa whole cell lysate using NB110-40693. USP33 was also immunoprecipitated by rabbit anti-USP33 antibody NB110-40692.

Immunohistochemistry: USP33 Antibody [NB110-40693] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- USP33 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Immunohistochemistry-Paraffin: USP33 Antibody [NB110-40693] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-USP33 used at a dilution of 1:250.

Immunohistochemistry-Paraffin: USP33 Antibody [NB110-40693] - FFPE section of mouse renal cell carcinoma. Affinity purified rabbit anti-USP33 used at a dilution of 1:250.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

USP33 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit USP33 Antibody - BSA Free (NB110-40693) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-USP33 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 900 and the C-terminus (residue 942) of human Ubiquitin Specific Peptidase 33 using the numbering given in entry NP_055832.3 (GeneID 23032).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: USP33
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:250
  • Immunohistochemistry-Paraffin 1:250
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for USP33 Antibody - BSA Free

• Deubiquitinating enzyme 33
• EC 3.1.2.15
• EC 3.4.19.12
• hVDU1
• KIAA1097
• KIAA1097MGC16868
• pVHL-interacting deubiquitinating enzyme 1
• ubiquitin carboxyl-terminal hydrolase 33
• ubiquitin specific peptidase 33
• ubiquitin thioesterase 33
• Ubiquitin thiolesterase 33
• Ubiquitin-specific-processing protease 33
• USP33
• VDU1
• VDU1ubiquitin specific protease 33
• VHL-interacting deubiquitinating enzyme 1

Background

A novel protein named VHL-interacting deubiquitinating enzyme 1 (VDU1/USP33) was identified as being able to directly interact with pVHL in vitro and in vivo. The VDU1-interacting region in pVHL is located in its beta-domain, and several naturally occurring mutations located in this domain disrupt the interaction between pVHL and VDU1 protein. VDU1 is able to be ubiquitinated via a pVHL-dependent pathway for proteasomal degradation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for USP33 Antibody (NB110-40693)(3)

Publications using NB110-40693

• Thorne C, Eccles RL, Coulson JM et al. Isoform-specific localization of the deubiquitinase USP33 to the Golgi apparatus. Traffic 2011-11-01 [PMID: 21801292]
• Yasunaga J, Lin FC, Lu X et al. Ubiquitin-specific peptidase 20 targets TRAF6 and human T cell leukemia virus type 1 tax to negatively regulate NF-kappaB signaling. J Virol 2011-07-01 [PMID: 21525354]
• Shenoy SK, Modi AS, Shukla AK et al. Beta-arrestin-dependent signaling and trafficking of 7-transmembrane receptors is reciprocally regulated by the deubiquitinase USP33 and the E3 ligase Mdm2. Proc Natl Acad Sci U S A 2009-04-01 [PMID: 19363159]

Bioinformatics

• Gene Symbol: USP33
• Entrez:
  • 23032(Human)
• Uniprot:
  • Q8TEY7(Human)