UCP1 Antibody (536435) [Phycoerythrin]

Human preadipocytes were stained with Mouse Anti-Human/Mouse UCP1 PE-conjugated Monoclonal Antibody (Catalog # IC6158P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

3T3-L1 mouse embryonic fibroblast adipose-like cell line was stained with Mouse Anti-Human/Mouse UCP1 PE-conjugated Monoclonal Antibody (Catalog # IC6158P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu, Mu
• Applications: Flow
• Clone: 536435
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

UCP1 Antibody (536435) [Phycoerythrin] Summary

• Immunogen: E. coli-derived recombinant human UCP1
  Met1-Thr307
  Accession # P25874
• Specificity: Detects human and mouse UCP1 in Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: UCP1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for UCP1 Antibody (536435) [Phycoerythrin]

• SLC25A7
• SLC25A7mitochondrial brown fat uncoupling protein 1
• Solute carrier family 25 member 7
• Thermogenin
• UCP 1
• UCP
• UCP1
• uncoupling protein 1 (mitochondrial, proton carrier)

Background

Mitochondrial brown fat uncoupling protein 1 (UCP1; also Thermogenin and UCP) is a 33 kDa member of the mitochondrial carrier family of proteins. Human and mouse UCP1 are both 307 amino acids (aa) in length and contain three solcar repetitive regions and six transmembrane segments. UCP1 is found in brown adipose tissue, where it becomes activated by fatty acids and inhibited by nucleotides. It functions as a mitochondrial transporter that creates a proton leak across the inner mitochondrial membrane, uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. Human and mouse UCP1 share 79% aa sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for UCP1 Antibody (IC6158P)(1)

Publication using IC6158P

• L Zhang, J Avery, A Yin, AM Singh, TS Cliff, H Yin, S Dalton Generation of Functional Brown Adipocytes from Human Pluripotent Stem Cells via Progression through a Paraxial Mesoderm State Cell Stem Cell, 2020-08-11;0(0):. 2020-08-11 [PMID: 32783886] (Western Blot, IHC, Flow Cytometry, ICC, Xenograft, Human)

Bioinformatics

• Gene Symbol: UCP1
• Uniprot:
  • P25874(Human)