U2AF2 Antibody - BSA Free

Immunohistochemistry-Paraffin: U2AF2 Antibody [NBP2-04140] - Human breast carcinoma. Antibody: Affinity purified rabbit anti- U2AF65 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Immunoprecipitation: U2AF2 Antibody [NBP2-04140] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-U2AF65 antibody NBP2-04138 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. U2AF65 was also immunoprecipitated by rabbit anti-U2AF65 antibodies NBP2-04139 and NBP2-04140, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

U2AF2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit U2AF2 Antibody - BSA Free (NBP2-04140) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-U2AF2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 430 and 475 of human U2 Small Nuclear Ribonucleoprotein Auxiliary Factor (65kD) using the numbering given in entry NP_009210.1 (GeneID 11338).
• Predicted Species: Mouse (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: U2AF2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for U2AF2 Antibody - BSA Free

• hU2AF(65)
• hU2AF65
• splicing factor U2AF 65 kD subunit
• splicing factor U2AF 65 kDa subunit
• U2 (RNU2) small nuclear RNA auxiliary factor 2
• U2 auxiliary factor 65 kDa subunit
• U2 small nuclear ribonucleoprotein auxiliary factor (65kD)
• U2 small nuclear RNA auxiliary factor 2
• U2AF65U2 snRNP auxiliary factor large subunit

Background

U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for U2AF2 Antibody (NBP2-04140)(1)

Publication using NBP2-04140

• Carolyn M Jablonowski, Waise Quarni, Shivendra Singh, Haiyan Tan, Dhanushka Hewa Bostanthirige, Hongjian Jin, Jie Fang, Ti-Cheng Chang, David Finkelstein, Ji-Hoon Cho, Dongli Hu, Vishwajeeth Pagala, Sadie Miki Sakurada, Shondra M Pruett-Miller, Ruoning Wang, Andrew Murphy, Kevin Freeman, Junmin Peng, Andrew M Davidoff, Gang Wu, Jun Yang, Sean Lawler, Wafik S El-Deiry Metabolic reprogramming of cancer cells by JMJD6-mediated pre-mRNA splicing associated with therapeutic response to splicing inhibitor eLife 2024-03-15 [PMID: 38488852]

Bioinformatics

• Gene Symbol: U2AF2
• Uniprot:
  • NP_009210.1(Human)