U2AF35 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: U2AF35 Antibody [NBP1-92553] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.

Immunohistochemistry-Paraffin: U2AF35 Antibody [NBP1-92553] - Staining of human hippocampus shows moderate nuclear positivity in neuronal cells.

Staining of human duodenum shows strong nuclear positivity in glandular cells.

Staining of human cerebral cortex shows strong nuclear positivity in neurons.

Staining of human skin shows strong nuclear positivity in squamous epithelial cells.

Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, Mycoplasma
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

U2AF35 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit U2AF35 Antibody - BSA Free (NBP1-92553) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-U2AF35 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: MHLKPISRELRRELYGRRRKKHRSRS
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: U2AF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  U2AF35 Protein (NBP1-92553PEP)

Reactivity Notes

Reactivity reported in scientific literature (PMID: 24579871)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for U2AF35 Antibody - BSA Free

• FP793
• human U2 snRNP auxiliary factor small subunit10U2AF35DKFZp313J1712
• RN
• RNU2AF1,35-KD subunit
• U2 auxiliary factor 35 kDa subunit
• U2 small nuclear RNA auxiliary factor 1
• U2 small nuclear RNA auxillary factor 1
• U2 snRNP auxiliary factor small subunit

Background

U2AF35 belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for U2AF35 Antibody (NBP1-92553)(1)

Publication using NBP1-92553

• Stadler C, Fagerberg L, Sivertsson A et al. RNA- and antibody-based profiling of the human proteome with focus on chromosome 19. J Proteome Res 2014-04-04 [PMID: 24579871]

Bioinformatics

• Gene Symbol: U2AF1