Tyrosine Hydroxylase Antibody (779427)

Images

 
Western blot shows lysates of human adrenal gland tissue, mouse adrenal gland tissue, and rat adrenal gland tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human/Mouse Tyrosine Hydroxylase Monoclonal ...read more
Simple Western lane view shows lysates of human adrenal gland tissue and mouse adrenal gland tissue, loaded at 0.2 mg/mL. Specific bands were detected for Tyrosine Hydroxylase at approximately 52-61 kDa (as indicated) ...read more
Tyrosine Hydroxylase was detected in immersion fixed mouse embryonic stem cells differentiated into dopaminergic neurons using Mouse Anti-Human/Mouse Tyrosine Hydroxylase Monoclonal Antibody (Catalog # MAB7566) at 10 ...read more
Tyrosine Hydroxylase was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Mouse Anti-Human/Mouse Tyrosine Hydroxylase Monoclonal Antibody (Catalog # MAB7566) at 25 µg/mL overnight ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF
Clone
779427
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Tyrosine Hydroxylase Antibody (779427) Summary

Immunogen
E. coli-derived recombinant human Tyrosine Hydroxylase
Ala278-Tyr401
Accession # P07101
Specificity
Detects human Tyrosine Hydroxylase in direct ELISAs and human, mouse and rat Tyrosine Hydroxylase in Western blots. In direct ELISAs, no cross-reactivity with recombinant human Tryptophan Hydroxylase-1 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
TH
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.25 ug/mL
  • Simple Western 5 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Immunocytochemistry 8-25 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 1 Review rated 5
using
MAB7566 in the following applications:

Publications
Read Publications using
MAB7566 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Tyrosine Hydroxylase Antibody (779427)

  • DYT14
  • DYT5b
  • EC 1.14.16
  • EC 1.14.16.2
  • TH
  • TYH dystonia 14
  • TYH
  • Tyrosine 3-hydroxylase
  • tyrosine 3-monooxygenase
  • Tyrosine Hydroxylase

Background

TH (Tyrosine 3-hydroxylase), also known as Tyrosine 3-monooxygenase, is a 60-62 kDa member of the biopterin-dependent aromatic amino acid hydroxylase family of molecules. It is expressed by neurons of the dopamine and autonomic nervous system, plus the neuroendocrine cells of the adrenal medulla. TH is considered the rate limiting enzyme for catecholamine synthesis, and serves to catalyze the hydroxylation of L-tyrosine. It maintains stores of catecholamines following secretion, and its activity is regulated by targeted site phosphorylation. Human TH is 528 amino acids (aa) in length. It contains an N-terminal ACT domain (aa 69-190) that binds small molecules and regulates enzyme activity, and a C-terminal enzymatic region (aa 196-493). There are three significant utilized phosphorylation sites. Two at Ser31 and Ser40 increase enzyme activity, while phosphorylation at Ser19 promotes subsequent Ser40 phosphorylation. TH functions as a 240 kDa noncovalent homotetramer. There are four potential splice variants. One shows a deletion of aa 31-34, a second shows a deletion of aa 31-61, while a third contains a Met substitution for aa 1-34. A fourth isoform variant shows a deletion of aa 35-61. Over aa 278-401, human TH shares 94% aa sequence identity with mouse TH, a molecule that most closely resembles the fourth human isoform variant described above.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Tyrosine Hydroxylase Antibody (MAB7566)(4)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC, Western Blot.


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ICC
(3)
Western Blot
(1)
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Human
(4)
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Review for Tyrosine Hydroxylase Antibody (MAB7566) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using MAB7566:
Filter by Applications
IHC-Fr
(1)
All Applications
Filter by Species
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Tyrosine Hydroxylase MAB7566
Enlarge
5
reviewed by:
Verified Customer
IHC-Fr Rat 09/02/2015
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedFrozen tissue sections or rat cerebrum
SpeciesRat
Lot2458991

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Video Protocols

WB Video Protocol

FAQs for Tyrosine Hydroxylase Antibody (MAB7566) (0)

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Blogs on Tyrosine Hydroxylase.

The identification of dopaminergic neurons using Tyrosine Hydroxylase in Parkinson's research and LRRK2
Tyrosine hydroxylase (TH) is a crucial enzyme involved in the biosynthesis of dopamine, norepinephrine and epinephrine in the brain.  Specifically, TH catalyzes the conversion of l-tyrosine to l-dihydroxyphenylalanine (l-dopa).  The importance of t...  Read full blog post.

Tyrosine hydroxylase - a marker for dopaminergic neurons in the central nervous system
Tyrosine hydroxylase is a member of the aromatic amino acid hydroxylase (AAAH) family.  It is expressed throughout the central nervous system (CNS) and catalyzes the conversion of tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), which can be, thr...  Read full blog post.

Tyrosine Hydroxylase - rate-limiting enzyme in catecholamine synthesis
Catecholamines are tyrosine-derived hormones that are produced in the adrenal gland. They include epinephrine, norepinephrine, and dopamine and are used as neurotransmitters by the central and peripheral nervous system. The rate limiting enzyme in...  Read full blog post.

A Big Guy for the Catecholamine Synthesis - Tyrosine hydroxylase (TH)
In the synthesis pathway for the catecholamines - dopamine, epinephrine, and norepinephrine, tyrosine hydroxylase is the rate-limiting enzyme. Through alternative mRNA splicing, a wide molecular diversity of TH isoforms are generated that are tissue-s...  Read full blog post.

Tyrosine Hydroxylase Deficiencies and Neurodegeneration
Tyrosine hydroxylase is the rate-limiting enzyme in the synthesis pathway of the catecholamines dopamine, epinephrine, and norepinephrine. Alternative mRNA splicing generates a wide molecular diversity of TH isoforms that are tissue specific and produ...  Read full blog post.

Tyrosine Hydroxylase Deficiency and Brain Disorders
 Tyrosine hydroxylase catalyzes the rate-limiting step in the biosynthesis of the catecholamines dopamine, norepinephrine, and epinephrine. A hallmark of Parkinson's disease is the loss of dopaminergic neurons in the substantia nigra. Mutations in cas...  Read full blog post.

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Recent Reviews

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5
1
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3
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2
0
1
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Verified Customer
09/02/2015
Application: IHC-Fr
Species: Rat

Bioinformatics

Gene Symbol TH
Uniprot