TSG-6 Overexpression Lysate (Native)

Images

 
Western Blot: TSG6 Overexpression Lysate (Adult Normal) [NBL1-17137] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TSG6.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

TSG-6 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC206510 and based on accession number NM_007115. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens tumor necrosis factor, alpha-induced protein 6 (TNFAIP6), mRNA.
Gene
TNFAIP6

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
28.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for TSG-6

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for TSG-6 Overexpression Lysate (Native)

  • Hyaluronate-binding protein
  • TNF alpha-induced protein 6
  • TNFAIP6
  • TNF-stimulated gene 6 protein
  • TSG6
  • TSG-6
  • TSG-6tumor necrosis factor alpha-inducible protein 6
  • TSG6tumor necrosis factor-inducible gene 6 protein
  • Tumor necrosis factor alpha-induced protein 6
  • tumor necrosis factor, alpha-induced protein 6
  • tumor necrosis factor-stimulated gene-6 protein

Background

The protein encoded by this gene is a secretory protein that contains a hyaluronan-binding domain, and thus is a member of the hyaluronan-binding protein family. The hyaluronan-binding domain is known to be involved in extracellular matrix stability and cell migration. This protein has been shown to form a stable complex with inter-alpha-inhibitor (I alpha I), and thus enhance the serine protease inhibitory activity of I alpha I, which is important in the protease network associated with inflammation. The expression of this gene can be induced by tumor necrosis factor alpha and interleukin-1. The expression can also be induced by mechanical stimuli in vascular smooth muscle cells, and is found to be correlated with proteoglycan synthesis and aggregation. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for TSG-6 Lysate (NBL1-17137) (0)

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Bioinformatics

Gene Symbol TNFAIP6
Entrez