PITX3 Antibody

Western Blot: PITX3 Antibody [NB100-96910] - (1ug/ml) staining of Human Cer(35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PITX3 Antibody Summary

• Description: Novus Biologicals Goat PITX3 Antibody (NB100-96910) is a polyclonal antibody validated for use in WB and ELISA. Anti-PITX3 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence PEHGCKGQEHSD corresponding to internal region according to NP_005020.1.
• Epitope: PEHGCKGQEHSD
• Marker: Mesencephalic Dopaminergic Neuronal Marker
• Specificity: This antibody is not excpected to cross-react with PITX1 and PITX2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PITX3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:1000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 40 kDa band observed in human brain is reported in scientific literature (PMID: 17848564).
• Control:

  PITX3 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PITX3 Antibody

• CTPP4
• Homeobox protein PITX3
• MGC12766
• paired-like homeodomain 3
• Paired-like homeodomain transcription factor 3
• pituitary homeobox 3
• PTX3

Background

This gene encodes a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. Members of this family act as transcription factors. This protein is involved in lens formation during eye development. Mutations of this gene have been associated with anterior segment mesenchymal dysgenesis and congenital cataracts. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PITX3 Antibody (NB100-96910)(2)

Publications using NB100-96910

• Coulon V, L'Honore A, Ouimette JF et al. A muscle-specific promoter directs Pitx3 gene expression in skeletal muscle cells. J Biol Chem 2007-11-01 [PMID: 17848564]
• Kim J, Inoue K, Ishii J et al. A MicroRNA feedback circuit in midbrain dopamine neurons. Science 2007-08-31 [PMID: 17761882]

Bioinformatics

• Gene Symbol: PITX3
• Entrez:
  • 5309(Human)
• Uniprot:
  • NP_005020.1(Human)