Epiregulin Antibody [Unconjugated]

Images

 
Recombinant Human Epiregulin (Catalog # 1195-EP) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human ...read more
Epiregulin was detected in immersion fixed paraffin-embedded sections of human skin using Goat Anti-Human Epiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1195) at 15 µg/mL overnight at 4 °C. ...read more
Recombinant Human Epiregulin protein was serially diluted 2-fold and captured by Rat Anti-Human Epiregulin Monoclonal Antibody (Catalog # MAB14252) coated on a Clear Polystyrene Microplate (Catalog # DY990). ...read more
EREG expression correlates with MMP-1 levels in human DCIS lesions. a qRT-PCR analysis of MMP-1 gene expression in MCF10A and MCF10DCIS cells. Expression levels of MMP-1 are normalized to CYBP. b Immunoblot analysis of ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Epiregulin Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Epiregulin (R&D Systems, Catalog # 1195-EP)
Val63-Leu108
Accession # O14944
Specificity
Detects human Epiregulin in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant mouse Epiregulin is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
EREG
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 0.3-1.5 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF1195 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Epiregulin Antibody [Unconjugated]

  • Epiregulin
  • ER
  • EREG
  • proepiregulin

Background

Epiregulin is a member of the EGF family of growth factors which includes, among others, epidermal growth factor (EGF), transforming growth factor (TGF)-alpha, amphiregulin (ARG), HB (heparin-binding)-EGF, betacellulin, and the various heregulins. All EGF family members are synthesized as transmembrane precursors and are converted to soluble forms by proteolytic cleavage. Epiregulin was originally purified from the mouse fibroblast-derived tumor cell line NIH3T3/T7. The human epiregulin cDNA encodes a 169 amino acid (aa) residues transmembrane precursor with a 29 aa signal peptide, a 21 aa transmembrane domain and a 21 aa cytoplasmic domain. The putative soluble mature Epiregulin comprising the EGF-like domain (aa residues 64-104) is formed by proteolytic removal of the propeptide regions. There is 85% aa sequence homology between human and mouse epiregulins. Epiregulin is expressed primarily in the placenta and macrophages. High level expression has also been detected in various carcinomas. Epiregulin specifically binds EGF R (ErbB1) and ErbB4 but not ErbB2 and ErbB3. It activates the homodimers of both ErbB1 and ErbB4. In addition, epiregulin can also activate all possible heteromeric combinations of the four ErbB family members. Epiregulin stimulates the proliferation of fibroblasts, smooth muscle cells and hepatocytes. It has been shown to be an autocrine growth factor for epidermal keratinocytes as well as mesangial cells. Epiregulin has also been shown to inhibit growth of several epithelial tumor cells. In addition, Epiregulin has been implicated in the implantation process during pregnancy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Epiregulin Antibody (AF1195)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 6 applications: ELISA Development, ICC, IHC, IHC-P, Neutralization, Western Blot.


Filter By Application
ELISA Development
(1)
ICC
(1)
IHC
(1)
IHC-P
(3)
Neutralization
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(7)
All Species
Showing Publications 1 - 7 of 7.
Publications using AF1195 Applications Species
S Liu, Y Wang, Y Han, W Xia, L Zhang, S Xu, H Ju, X Zhang, G Ren, L Liu, W Ye, Z Zhang, J Hu EREG-driven oncogenesis of Head and Neck Squamous Cell Carcinoma exhibits higher sensitivity to Erlotinib therapy Theranostics, 2020-08-25;10(23):10589-10605. 2020-08-25 [PMID: 32929368] (IHC, Human) IHC Human
Tumor endothelial cells promote metastasis and cancer stem cell-like phenotype through elevated Epiregulin in esophageal cancer Am J Cancer Res, 2016-10-01;6(10):2277-2288. 2016-10-01 [PMID: 27822417] (IHC-P, Western Blot, Human) IHC-P, Western Blot Human
Auf G, Jabouille A, Delugin M, Guerit S, Pineau R, North S, Platonova N, Maitre M, Favereaux A, Vajkoczy P, Seno M, Bikfalvi A, Minchenko D, Minchenko O, Moenner M High epiregulin expression in human U87 glioma cells relies on IRE1alpha and promotes autocrine growth through EGF receptor. BMC Cancer, 2013-12-13;13(0):597. 2013-12-13 [PMID: 24330607] (ELISA Development, Human) ELISA Development Human
Amsterdam A, Shezen E, Raanan C Epiregulin as a marker for the initial steps of ovarian cancer development. Int. J. Oncol., 2011-07-14;39(5):1165-72. 2011-07-14 [PMID: 21769422] (IHC-P, Human) IHC-P Human
Sun M, Behrens C, Feng L, Ozburn N, Tang X, Yin G, Komaki R, Varella-Garcia M, Hong WK, Aldape KD, Wistuba II HER family receptor abnormalities in lung cancer brain metastases and corresponding primary tumors. Clin. Cancer Res., 2009-07-21;15(15):4829-37. 2009-07-21 [PMID: 19622585] (IHC-P, Human) IHC-P Human
Armant DR, Kilburn BA, Petkova A, Edwin SS, Duniec-Dmuchowski ZM, Edwards HJ, Romero R, Leach RE Human trophoblast survival at low oxygen concentrations requires metalloproteinase-mediated shedding of heparin-binding EGF-like growth factor. Development, 2006-01-11;133(4):751-9. 2006-01-11 [PMID: 16407398] (ICC, Human) ICC Human
Kansra S, Johnson JL Autocrine extracellular signal-regulated kinase (ERK) activation in normal human keratinocytes: metalloproteinase-mediated release of amphiregulin triggers signaling from ErbB1 to ERK. Mol. Biol. Cell, 2004-07-14;15(9):4299-309. 2004-07-14 [PMID: 15254267] (Neutralization, Human) Neutralization Human

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Bioinformatics

Gene Symbol EREG
Uniprot