Mouse TSG-6 ELISA Kit (Colorimetric)

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Standard Curve Range: 0.156 - 10 ng/mL (example only; lot dependent)
• Sensitivity: 0.054 ng/mL (example only; lot dependent)

Mouse TSG-6 ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 4.5 hours
• Standard Curve Range: 0.156 - 10 ng/mL (example only; lot dependent)
• Sensitivity: 0.054 ng/mL (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 12 (example only; lot dependent)
• Intra-Assay: %CV < 10 (example only; lot dependent)
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: TNFAIP6

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse TSG-6 ELISA Kit (Colorimetric)

• Hyaluronate-binding protein
• TNF alpha-induced protein 6
• TNFAIP6
• TNF-stimulated gene 6 protein
• TSG6
• TSG-6
• TSG-6tumor necrosis factor alpha-inducible protein 6
• TSG6tumor necrosis factor-inducible gene 6 protein
• Tumor necrosis factor alpha-induced protein 6
• tumor necrosis factor, alpha-induced protein 6
• tumor necrosis factor-stimulated gene-6 protein

Background

TSG6 is encoded by this gene is a secretory protein that contains a hyaluronan-binding domain, and thus is a member of the hyaluronan-binding protein family. The hyaluronan-binding domain is known to be involved in extracellular matrix stability and cell migration. This protein has been shown to form a stable complex with inter-alpha-inhibitor (I alpha I), and thus enhance the serine protease inhibitory activity of I alpha I, which is important in the protease network associated with inflammation. The expression of this gene can be induced by tumor necrosis factor alpha and interleukin-1. The expression can also be induced by mechanical stimuli in vascular smooth muscle cells, and is found to be correlated with proteoglycan synthesis and aggregation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for TSG-6 ELISA Kit (NBP3-53731). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: TNFAIP6