TRPM4 Antibody (OTI10H5) - Azide and BSA Free

Western Blot: TRPM4 Antibody (OTI10H5) - Azide and BSA Free [NBP2-74647] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRPM4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRPM4.

Immunohistochemistry: TRPM4 Antibody (OTI10H5) - Azide and BSA Free [NBP2-74647] - Staining of paraffin-embedded Human Ovary tissue using anti-TRPM4 mouse monoclonal antibody.

Western Blot: TRPM4 Antibody (OTI10H5) - Azide and BSA Free [NBP2-74647] - Analysis of extracts (35ug) from 9 different cell lines by using anti-TRPM4 monoclonal antibody.

Immunohistochemistry: TRPM4 Antibody (OTI10H5) - Azide and BSA Free [NBP2-74647] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-TRPM4 mouse monoclonal antibody.

Immunohistochemistry: TRPM4 Antibody (OTI10H5) - Azide and BSA Free [NBP2-74647] - Staining of paraffin-embedded Human Kidney tissue using anti-TRPM4 mouse monoclonal antibody.

Immunohistochemistry: TRPM4 Antibody (OTI10H5) - Azide and BSA Free [NBP2-74647] - Staining of paraffin-embedded Human liver tissue using anti-TRPM4 mouse monoclonal antibody.

Immunohistochemistry: TRPM4 Antibody (OTI10H5) - Azide and BSA Free [NBP2-74647] - Staining of paraffin-embedded Human pancreas tissue using anti-TRPM4 mouse monoclonal antibody.

• Reactivity: Hu, Ca, Pm, Pm
• Applications: WB, IHC
• Clone: OTI10H5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

TRPM4 Antibody (OTI10H5) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse TRPM4 Antibody (OTI10H5) - Azide and BSA Free (NBP1-48036) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human TRPM4 (NP_060106) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TRPM4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin
  • Western Blot 1:5000-10000
• Theoretical MW: 134.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for TRPM4 Antibody (OTI10H5) - Azide and BSA Free

• Calcium-activated non-selective cation channel 1
• FLJ20041
• hTRPM4
• Long transient receptor potential channel 4
• LTrpC4
• LTrpC-4
• Melastatin-4
• PFHB1B
• transient receptor potential cation channel subfamily M member 4
• transient receptor potential cation channel, subfamily M, member 4
• TRPM4
• TRPM4B

Background

FUNCTION: Calcium-activated non selective (CAN) cation channel that mediates membrane depolarization. While it is activated by increase in intracellular Ca(2+), it is impermeable to it. Mediates transport of monovalent cations (Na(+) > K(+) > Cs(+) > Li(+)), leading to depolarize the membrane. It thereby plays a central role in cadiomyocytes, neurons from entorhinal cortex, dorsal root and vomeronasal neurons, endocrine pancreas cells, kidney epithelial cells, cochlea hair cells etc. Participates in T-cell activation by modulating Ca(2+) oscillations after T lymphocyte activation, which is required for NFAT-dependent IL2 production. Involved in myogenic constriction of cerebral arteries. Controls insulin secretion in pancreatic beta-cells. May also be involved in pacemaking or could cause irregular electrical activity under conditions of Ca(2+) overload.; ENZYME REGULATION: Gating is voltage-dependent and repressed by decavanadate. Calmodulin-binding confers the Ca(2+) sensitivity. ATP is able to restore Ca(2+) sensitivity after desensitization. Phosphatidylinositol 4,5-biphosphate (PIP2)-binding strongly enhances activity, by increasing the channel's Ca(2+) sensitivity and shifting its voltage dependence of activation towards negative potentials. Activity is also enhanced by 3,5-bis(trifluoromethyl)pyrazole derivative (BTP2). SUBUNIT: Homomultimer. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Widely expressed with a high expression in intestine and prostate. In brain, it is both expressed in whole cerebral arteries and isolated vascular smooth muscle cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRPM4