TRPM4 Antibody (OTI10H5)

Images

 
Western Blot: TRPM4 Antibody (OTI10H5) [NBP1-48036] - Analysis of extracts (35ug) from 9 different cell lines by using anti-TRPM4 monoclonal antibody.
Immunohistochemistry-Paraffin: TRPM4 Antibody (OTI10H5) [NBP1-48036] - Human endometrial carcinoma tissue section. Fixed paraffin embedded sections were deparaffinized. EDTA was used for antigen retrieval. Samples were ...read more
Western Blot: TRPM4 Antibody (10H5) [NBP1-48036] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRPM4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunohistochemistry-Paraffin: TRPM4 Antibody (10H5) [NBP1-48036] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-TRPM4 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TRPM4 Antibody (10H5) [NBP1-48036] - Staining of paraffin-embedded Human Kidney tissue using anti-TRPM4 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TRPM4 Antibody (10H5) [NBP1-48036] - Staining of paraffin-embedded Human liver tissue using anti-TRPM4 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TRPM4 Antibody (10H5) [NBP1-48036] - Staining of paraffin-embedded Human Ovary tissue using anti-TRPM4 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TRPM4 Antibody (10H5) [NBP1-48036] - Staining of paraffin-embedded Human pancreas tissue using anti-TRPM4 mouse monoclonal antibody.
Immunohistochemistry: TRPM4 Antibody (OTI10H5) [NBP1-48036] - Human heart tissue was blocked with 10% serum and stained with the primary antibody at 1:100 dilution overnight at 4 degrees. A secondary FITC-conjugated ...read more

Product Details

Summary
Reactivity Hu, Ca, Pm, PmSpecies Glossary
Applications WB, IHC, IHC-P
Clone
OTI10H5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

TRPM4 Antibody (OTI10H5) Summary

Immunogen
Full length human recombinant protein of human TRPM4 (NP_060106) produced in HEK293T cell.
Specificity
This antibody is specific for Homo sapiens transient receptor potential cation channel, subfamily M, member 4 (TRPM4).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TRPM4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:5000-10000
Theoretical MW
134.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 5
using
NBP1-48036 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TRPM4 Antibody (OTI10H5)

  • Calcium-activated non-selective cation channel 1
  • FLJ20041
  • hTRPM4
  • Long transient receptor potential channel 4
  • LTrpC4
  • LTrpC-4
  • Melastatin-4
  • PFHB1B
  • transient receptor potential cation channel subfamily M member 4
  • transient receptor potential cation channel, subfamily M, member 4
  • TRPM4
  • TRPM4B

Background

Calcium-activated non selective (CAN) cation channel that mediates membrane depolarization. While it is activated by increase in intracellular Ca(2+), it is impermeable to it. Mediates transport of monovalent cations (Na(+) > K(+) > Cs(+) > Li(+)), leading to depolarize the membrane. It thereby plays a central role in cadiomyocytes, neurons from entorhinal cortex, dorsal root and vomeronasal neurons, endocrine pancreas cells, kidney epithelial cells, cochlea hair cells etc. Participates in T-cell activation by modulating Ca(2+) oscillations after T lymphocyte activation, which is required for NFAT-dependent IL2 production. Involved in myogenic constriction of cerebral arteries. Controls insulin secretion in pancreatic beta-cells. May also be involved in pacemaking or could cause irregular electrical activity under conditions of Ca(2+) overload

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-31133
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-88370
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-48760
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-12906
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, MiAr, WB
NB100-98844
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, WB
NB110-81601
Species: Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
NBP1-77260
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NBP1-77262
Species: Hu, Mu
Applications: ELISA, IF, IHC, IHC-P, WB
NBP1-97311
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KD, WB
NB110-74960
Species: Hu, Pm, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NB100-1617
Species: Hu, Mu, Pm, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
DCLU00
Species: Hu
Applications: ELISA
NBP1-32096
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-58415
Species: Hu
Applications: IHC, IHC-P
NB100-98864
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
MAB1417
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
NB110-40763
Species: Gp, Hu, Mu, Rt, Ze
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, WB
NBP1-20989
Species: Hu, Rt
Applications: IHC, IHC-P, PEP-ELISA, WB

Publications for TRPM4 Antibody (NBP1-48036) (0)

There are no publications for TRPM4 Antibody (NBP1-48036).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for TRPM4 Antibody (NBP1-48036) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NBP1-48036:
Filter by Applications
Immunohistochemistry-Paraffin
(1)
IHC/Immunofluorescence
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin TRPM4 NBP1-48036
Enlarge
5
reviewed by:
Michael Nowak
Immunohistochemistry-Paraffin Human 12/13/2021
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedEndometrial carcinoma
SpeciesHuman
LotOTI10H5

Comments

CommentsFixed paraffin embedded sections were deparaffinized. EDTA was used for antigen retrieval. Samples were incubated O/N in 4 C with the primary antibody (1:200 dilution).
Other TRPM4 NBP1-48036
Enlarge
5
reviewed by:
Ashleigh Dean
IHC/Immunofluorescence Human 08/27/2021
View

Summary

ApplicationOther
Sample TestedHeart tissue
SpeciesHuman
LotOTI10H5

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TRPM4 Antibody (NBP1-48036) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional TRPM4 Products

Bioinformatics Tool for TRPM4 Antibody (NBP1-48036)

Discover related pathways, diseases and genes to TRPM4 Antibody (NBP1-48036). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for TRPM4 Antibody (NBP1-48036)

Discover more about diseases related to TRPM4 Antibody (NBP1-48036).
 

Pathways for TRPM4 Antibody (NBP1-48036)

View related products by pathway.

PTMs for TRPM4 Antibody (NBP1-48036)

Learn more about PTMs related to TRPM4 Antibody (NBP1-48036).
 

Research Areas for TRPM4 Antibody (NBP1-48036)

Find related products by research area.

Blogs on TRPM4.

Winter is coming, and TRPM8 welcomes the cold!
TRPM8, or transient receptor potential melastatin 8, is a nonselective cation channel that is activated by cold environments and menthol-like cooling compounds.  While TRPM8 is best known for its location in peripheral nerve endings, it has functio...  Read full blog post.

Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Michael Nowak
12/13/2021
Application: Immunohistochemistry-Paraffin
Species: Human

Ashleigh Dean
08/27/2021
Application: IHC/Immunofluorescence
Species: Human

Bioinformatics

Gene Symbol TRPM4
Entrez