Western Blot: TRPA1 Antibody [NB110-40763] - Expression and localization of TRPA1 channels in wild-type (WT) and APP/PS1 Tg mice. Brains were harvested from WT and APP/PS1 Tg mice at 8A months old. Western blot analysis ...read more
Immunohistochemistry: TRPA1 Antibody [NB110-40763] - Staining TRPA1 in mouse intestine.
Immunocytochemistry/ Immunofluorescence: TRPA1 Antibody [NB110-40763] - Ai2 elicits TRPA1-dependent Ca2+ influx in astrocytes. Immunostaining of primary astrocytes from WT mice with anti-IgG and anti-TRPA1 antibody, ...read more
Flow Cytometry: TRPA1 Antibody [NB110-40763] - An intracellular stain was performed on A549 cells with NB110-40763C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized ...read more
Flow Cytometry: TRPA1 Antibody [NB110-40763] - An intracellular stain was performed on A549 cells with NB110-40763G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized ...read more
Western Blot: TRPA1 Antibody [NB110-40763] - The essential role of TRPA1 in Ai2-induced calcium influx in HEK293 cells. (a) Western blot analysis of TRPA1 and I+--tubulin protein levels in non-treated, vector-or ...read more
Immunocytochemistry/ Immunofluorescence: TRPA1 Antibody [NB110-40763] - Analysis of TRPA1 in HEK 293 cells (Flp In Trex system) cells.
This TRPA1 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Western blot analysis. Additional modified form of TRPA1 can also be detected. Immunohistochemistry-Frozen was reported in scientific literature. Use in FLOW reported in scientific literature (PMID: 28990934). Use in ELISA reported in scientific literature (PMID: 29803505).
127.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 2 Reviews rated 4.5 using NB110-40763 in the following applications:
Rat reactivity reported in scientific literature (PMID: 27748654). Zebrafish reactivity reported from a verified customer review.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
Immunogen affinity purified
Alternate Names for TRPA1 Antibody - BSA Free
ANKTM1ankyrin-like with transmembrane domains 1
Ankyrin-like with transmembrane domains protein 1
Transformation-sensitive protein p120
transient receptor potential cation channel subfamily A member 1
transient receptor potential cation channel, subfamily A, member 1
TRPA1 (transient receptor potential cation channel subfamily A member 1; originally named as ANKTM1) is a member of transient receptor potential ion channels (TRPCs) which responds to stimuli such as cold temperatures, pungent compounds, and plays role in both nociceptor and hair cell transductions. TRPA1 is activated by variety of mechanical and chemicals stimuli including dietary irritants such as isothyocyanates (found in mustard oil, horse-radish, wasabi), allycin/garlic etc. and its activity is regulated by endogenous pro-inflammatory factors such as cyclopentane prostaglandins and byproducts of oxidative stress (e.g. 4-HNE, 4-oxononenal) which activate TRPA1 directly via covalent modification of its cysteine residues. Once TRPA1 is activated, increased intracellular Ca2+ induces the peripheral release of neuropeptides namely substance P and CGRP (calcitonin gene-related peptide), purines, and other transmitters from sensitized nerve endings, which results in neurogenic inflammation and hypersensitivity. TRPA1 is mainly expressed in small-diameter peptidergic nociceptors of the dorsal root, nodose and trigeminal ganglia, along with TRPV1, and has also been reported to be expressed in non-neuronal tissues (skeletal muscle, lung, small intestine, colon, pancreas etc) and is found dysregulated during process of carcinogenesis in certain malignancies.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Liu Q, Feng L, Han X et al. The TRPA1 Channel Mediates Mechanical Allodynia and Thermal Hyperalgesia in a Rat Bone Cancer Pain Model Frontiers in Pain Research Mar 22 2021 (WB, Rat)
Zhu T, Niu J, Su C et al. Botulinum Neurotoxin A Prevents the Development of Nitroglycerin-Induced Chronic Migraine via Inhibition of CGRP and NLRP3 Inflammasomes in Mice Research Square Jun 18 2020 (WB, Mouse)
Nummenmaa E TRPA1 as a Novel Factor and Drug Target in Osteoarthritis Thesis Jan 1 2020 (WB, Human)
1. ZEM-2S cells were harvested using Tyrode/EDTA solution; 2. Cells were fixed in 4% paraformaldehyde for 30 min at 4 oC; 3. Cells were washed with PBS and centrifuged at 2000 g for 3 min; 4. Approximately 10^6 cells were added to round-bottom plates and centrifuged; 5. Blocking solution (1% BSA, 22.52 mg/ml glycine in PBS) was added to each well, and cells were kept at 4oC for 30 min; 6. Cells were washed with PBS and incubated with rabbit TRPA1 mouse antibody (NB110-40763) in incubation solution (for each 100 mL 0.25 g of carragenine, 1 g of BSA, and 300 uL of Triton X-100). Cells were incubated overnight. 7. Cells were washed with PBS, centrifuged, and secondary antibody (goat-anti rabbit Alexa 488 - 1:2000) was added at room temperature for 60 min; 8. Cells were washed with PBS and harvested. 9. Acquisition took place in FACS Canto (BD Biosciences)
FAQs for TRPA1 Antibody (NB110-40763). (Showing 1 - 4 of 4 FAQs).
I read the first publication (Yu S et al. 2009) referenced for NB110-40763. This product reacts with human and mouse according to your datasheet, but in that publication it was used for western blot in guinea pig. Does this mean this antibody is suitable to use for guinea pig samples?
Yes, according to this paper this antibody has been successfully used on guinea pig samples. This is the only testing that has been done in guinea pig though as we have done no in-house testing. It should work just fine for you.
Has the specificity of NB110-40763 has been confirmed by mouse dorsal root ganglion (DRG) tissue in immunohistochemistry? Do you know what sample types have been tested?
We have a couple of publications with the base product catalog number NB110-40763 (which is just the unconjugated version of NB110-40763G), which have utilized our product with mouse colonic dorsal root ganglion. (PMID 19875705 and PMID 21689654). The image on our website was taken of a mouse intestine sample.
What are the other two bands in the image on the datasheet? UniProt finds a theoretical MW of 127.5kDa. You find a MW of 110kDa in WB application. There are two more bands at 60kDa and 17kDa. What are these? Unspecific staining or fragments or variants of the protein? I did not find any info on variants when looking into the UniProt data (http://www.uniprot.org/uniprot/O75762).
We generally use gradient gels in our lab and for proteins which are multi-pass membrane protein, it is not uncommon to see an approximate 10-15% change in expected vs observed molecular weight in WB assay. The band observed at approximately 110kD is highly specific and there is no doubt that the antibody is detecting the right band. No, we have not characterized the additional low molecular weight bands yet which are considerably mild bands when compared to the expected target band. During a brief literature research this morning, I came across some papers wherein other researchers have also observed additional lower molecular weight bands and some of those references are: J Biol Chem. 2010 May 14; 285(20): 15167-15177; J Biol Chem. 2006 October 27; 281(43): 32879-32890; J Clin Invest. 2007 July 2; 117(7): 1979-1987.
Human brain membrane fraction is mentioned to be used as a positive control for this antibody. Do you sell the positive control too?
The Western blot data that is shown on our product page for the TRPA1 antibody with catalogue number NB110-40763 was, as you have pointed out, generated from human brain membrane lysate. We do sell a human brain membrane fraction that is suitable for Western blotting, and you can see this here: TRPA1 antibody datasheet. This lysate is supplied as native protein, and we recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.
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