TRPA1 Antibody


Western Blot: TRPA1 Antibody [NB110-40763] - Expression and localization of TRPA1 channels in wild-type (WT) and APP/PS1 Tg mice. Brains were harvested from WT and APP/PS1 Tg mice at 8A months old. Western blot analysis more
Immunohistochemistry: TRPA1 Antibody [NB110-40763] - Staining TRPA1 in mouse intestine.
Immunocytochemistry/ Immunofluorescence: TRPA1 Antibody [NB110-40763] - Ai2 elicits TRPA1-dependent Ca2+ influx in astrocytes. Immunostaining of primary astrocytes from WT mice with anti-IgG and anti-TRPA1 antibody, more
Flow Cytometry: TRPA1 Antibody [NB110-40763] - An intracellular stain was performed on A549 cells with NB110-40763C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized more
Flow Cytometry: TRPA1 Antibody [NB110-40763] - An intracellular stain was performed on A549 cells with NB110-40763G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized more
Western Blot: TRPA1 Antibody [NB110-40763] - The essential role of TRPA1 in Ai2-induced calcium influx in HEK293 cells. (a) Western blot analysis of TRPA1 and I+--tubulin protein levels in non-treated, vector-or more
Immunocytochemistry/ Immunofluorescence: TRPA1 Antibody [NB110-40763] - Analysis of TRPA1 in HEK 293 cells (Flp In Trex system) cells.

Product Details

Reactivity Hu, Mu, Rt, Gp, ZeSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
1.0 mg/ml

Order Details

TRPA1 Antibody Summary

A synthetic peptide made to a region within the N-terminus (residues 1-100) of the human TRPA1 protein. [Swiss-Prot# O75762]
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 2 ug/ml
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:200
  • Immunohistochemistry 0.5-1.0 ug/ml
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:250
Application Notes
This TRPA1 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Western blot analysis, where a band is seen at ~110 kDa. Immunohistochemistry-Frozen was reported in scientific literature. Use in FLOW reported in scientific literature (PMID: 28990934). Use in ELISA reported in scientific literature (PMID: 29803505).
Reviewed Applications
Read 2 Reviews rated 4.5
NB110-40763 in the following applications:

Read Publications using
NB110-40763 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 27748654). Zebrafish reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for TRPA1 Antibody

  • ANKTM1ankyrin-like with transmembrane domains 1
  • Ankyrin-like with transmembrane domains protein 1
  • Transformation-sensitive protein p120
  • transient receptor potential cation channel subfamily A member 1
  • transient receptor potential cation channel, subfamily A, member 1


TRPA1 (transient receptor potential cation channel subfamily A member 1; originally named as ANKTM1) is a member of transient receptor potential ion channels (TRPCs) which responds to stimuli such as cold temperatures, pungent compounds, and plays role in both nociceptor and hair cell transductions. TRPA1 is activated by variety of mechanical and chemicals stimuli including dietary irritants such as isothyocyanates (found in mustard oil, horse-radish, wasabi), allycin/garlic etc. and its activity is regulated by endogenous pro-inflammatory factors such as cyclopentane prostaglandins and byproducts of oxidative stress (e.g. 4-HNE, 4-oxononenal) which activate TRPA1 directly via covalent modification of its cysteine residues. Once TRPA1 is activated, increased intracellular Ca2+ induces the peripheral release of neuropeptides namely substance P and CGRP (calcitonin gene-related peptide), purines, and other transmitters from sensitized nerve endings, which results in neurogenic inflammation and hypersensitivity. TRPA1 is mainly expressed in small-diameter peptidergic nociceptors of the dorsal root, nodose and trigeminal ganglia, along with TRPV1, and has also been reported to be expressed in non-neuronal tissues (skeletal muscle, lung, small intestine, colon, pancreas etc) and is found dysregulated during process of carcinogenesis in certain malignancies.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TRPA1 Antibody (NB110-40763)(39)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Guinea Pig.

We have publications tested in 7 applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, WB.

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Showing Publications 1 - 10 of 39. Show All 39 Publications.
Publications using NB110-40763 Applications Species
Oguri G, Nakajima T, Kikuchi H et al. Allyl isothiocyanate (AITC) activates nonselective cation currents in human cardiac fibroblasts: possible involvement of TRPA1 Heliyon Jan 1 2021 [PMID: 33458442] (WB, Human) WB Human
Van Liefferinge E, Van Noten N, Degroote J et al. Expression of Transient Receptor Potential Ankyrin 1 and Transient Receptor Potential Vanilloid 1 in the Gut of the Peri-Weaning Pig Is Strongly Dependent on Age and Intestinal Site Animals : an open access journal from MDPI Dec 17 2020 [PMID: 33348615] (IHC) IHC
Wang S, Qi S, Kogure Y et al. The ubiquitin E3 ligase Nedd4-2 relieves mechanical allodynia through the ubiquitination of TRPA1 channel in db/db mice The European journal of neuroscience Nov 25 2020 [PMID: 33236491]
Chen Z, Luo J, Li J et al. Interleukin-33 Promotes Serotonin Release from Enterochromaffin Cells for Intestinal Homeostasis Immunity Nov 10 2020 [PMID: 33220232]
Li Q, Ma TL, Qiu YQ et al. Connexin 36 Mediates Orofacial Pain Hypersensitivity Through GluK2 and TRPA1 Neurosci Bull Oct 16 2020 [PMID: 33067780]
Virk HS, Rekas MZ, Biddle MS, et al. Validation of antibodies for the specific detection of human TRPA1 Sci Rep Dec 6 2019 [PMID: 31811235] (WB, ICC/IF, Human) WB, ICC/IF Human
Sahoo SS, Majhi RK, Tiwari A et al. Transient receptor potential ankyrin1 channel is endogenously expressed in T cells and is involved in immune functions Biosci. Rep. Sep 30 2019 [PMID: 31488616] (ICC/IF, Mouse, Human) ICC/IF Mouse, Human
Brandolini L, Castelli V, Aramini A et al. DF2726A, a new IL-8 signalling inhibitor, is able to counteract chemotherapy-induced neuropathic pain Sci Rep Aug 13 2019 [PMID: 31409858] (WB, Rat) WB Rat
Ma S, Zhang Y, He K et al. Knockout of TRPA1 exacerbates angiotensin II-induced kidney injury Am. J. Physiol. Renal Physiol. Jul 24 2019 [PMID: 31339777]
Miao, F;Wang, R;Cui, G;Li, X;Wang, T;Li, X; Engagement of MicroRNA-155 in Exaggerated Oxidative Stress Signal and TRPA1 in the Dorsal Horn of the Spinal Cord and Neuropathic Pain During Chemotherapeutic Oxaliplatin Neurotox Res Apr 23 2019 [PMID: 31016687] (WB, Rat) WB Rat
Show All 39 Publications.

Reviews for TRPA1 Antibody (NB110-40763) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Zebrafish

Reviews using NB110-40763:
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Images Ratings Applications Species Date Details
Flow Cytometry TRPA1 NB110-40763
reviewed by:
Flow Zebrafish


ApplicationFlow Cytometry
Sample TestedZEM2S,ZEM2S (ATCC® CRL-2147™)
LotF-4 Dt


Comments1.     ZEM-2S cells were harvested using Tyrode/EDTA solution;
2.     Cells were fixed in 4% paraformaldehyde for 30 min at 4 oC;
3.     Cells were washed with PBS and centrifuged at 2000 g for 3 min;
4.     Approximately 10^6 cells were added to round-bottom plates and centrifuged;
5.     Blocking solution (1% BSA, 22.52 mg/ml glycine in PBS) was added to each well, and cells were kept at 4oC for 30 min;
6.     Cells were washed with PBS and incubated with rabbit TRPA1 mouse antibody (NB110-40763) in incubation solution (for each 100 mL 0.25 g of carragenine, 1 g of BSA, and 300 uL of Triton X-100). Cells were incubated overnight.
7.     Cells were washed with PBS, centrifuged, and secondary antibody (goat-anti rabbit Alexa 488 - 1:2000) was added at room temperature for 60 min;
8.     Cells were washed with PBS and harvested.
9.     Acquisition took place in FACS Canto (BD Biosciences)
Immunofluorescence TRPA1 NB110-40763
reviewed by:
TinaMarie Lieu
IF Human 08/21/2012


Sample TestedHEKFLPNTREX Cell line
CommentsTRP channel antibodies are difficult to stain with specificity. This TRPA1 antibody from Novus works pretty well.


Blocking DetailsBlock with 10% goat serum in 1% BSA & 0.1% Saponin for 2 hrs at RT

Primary Anitbody

Dilution RatioPrimary antibody of TRPA1 from Novus Biologicals – dilute in 1% BSA/0.1% Saponin (1:200 dilution)

Secondary Antibody

Secondary DescriptionSecondary antibody diluted in 1%BSA/0.1% Saponin (1:1000 dilution) – Invitrogen Alexa Flour Goat anti-rabbit
Secondary Concentration1:1000


Detection NotesLeica with MetaFlour Flourescene Ratio Imaging, Pos. Control (DRG expressed TRPA1), Neg. Control (HEK nontransfected with TRPA1)
Fixation DetailsFixed in cold methanol for 2 minutes (4C)
Wash DescriptionPBS Wash 3X


CommentsTRP channel antibodies are difficult to stain with specificity. This TRPA1 antibody from Novus works pretty well.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TRPA1 Antibody (NB110-40763). (Showing 1 - 4 of 4 FAQs).

  1. I read the first publication (Yu S et al. 2009) referenced for NB110-40763. This product reacts with human and mouse according to your datasheet, but in that publication it was used for western blot in guinea pig. Does this mean this antibody is suitable to use for guinea pig samples?
    • Yes, according to this paper this antibody has been successfully used on guinea pig samples. This is the only testing that has been done in guinea pig though as we have done no in-house testing. It should work just fine for you.
  2. Has the specificity of NB110-40763 has been confirmed by mouse dorsal root ganglion (DRG) tissue in immunohistochemistry? Do you know what sample types have been tested?
    • We have a couple of publications with the base product catalog number NB110-40763 (which is just the unconjugated version of NB110-40763G), which have utilized our product with mouse colonic dorsal root ganglion. (PMID 19875705 and PMID 21689654). The image on our website was taken of a mouse intestine sample.
  3. What are the other two bands in the image on the datasheet? UniProt finds a theoretical MW of 127.5kDa. You find a MW of 110kDa in WB application. There are two more bands at 60kDa and 17kDa. What are these? Unspecific staining or fragments or variants of the protein? I did not find any info on variants when looking into the UniProt data (
    • We generally use gradient gels in our lab and for proteins which are multi-pass membrane protein, it is not uncommon to see an approximate 10-15% change in expected vs observed molecular weight in WB assay. The band observed at approximately 110kD is highly specific and there is no doubt that the antibody is detecting the right band. No, we have not characterized the additional low molecular weight bands yet which are considerably mild bands when compared to the expected target band. During a brief literature research this morning, I came across some papers wherein other researchers have also observed additional lower molecular weight bands and some of those references are: J Biol Chem. 2010 May 14; 285(20): 15167-15177; J Biol Chem. 2006 October 27; 281(43): 32879-32890; J Clin Invest. 2007 July 2; 117(7): 1979-1987.
  4. Human brain membrane fraction is mentioned to be used as a positive control for this antibody. Do you sell the positive control too?
    • The Western blot data that is shown on our product page for the TRPA1 antibody with catalogue number NB110-40763 was, as you have pointed out, generated from human brain membrane lysate. We do sell a human brain membrane fraction that is suitable for Western blotting, and you can see this here: TRPA1 antibody datasheet. This lysate is supplied as native protein, and we recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NB110-40763AF350
Alexa Fluor 405 NB110-40763AF405
Alexa Fluor 488 NB110-40763AF488
Alexa Fluor 532 NB110-40763AF532
Alexa Fluor 594 NB110-40763AF594
Alexa Fluor 647 NB110-40763AF647
Alexa Fluor 700 NB110-40763AF700
Alexa Fluor 750 NB110-40763AF750
Allophycocyanin/Cy7 NB110-40763APCCY7
Biotin NB110-40763B
DyLight 350 NB110-40763UV
DyLight 405 NB110-40763V
DyLight 488 NB110-40763G
DyLight 550 NB110-40763R
DyLight 594 NB110-40763DL594
DyLight 650 NB110-40763C
DyLight 680 NB110-40763FR
DyLight 755 NB110-40763IR
FITC NB110-40763F
HRP NB110-40763H
Janelia Fluor 549 NB110-40763JF549
Janelia Fluor 646 NB110-40763JF646
PE/Atto594 NB110-40763PEATT594
PE/Cy5.5 NB110-40763PECY55
PE/Cy7 NB110-40763PECY7

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Blogs on TRPA1.

Winter is coming, and TRPM8 welcomes the cold!
TRPM8, or transient receptor potential melastatin 8, is a nonselective cation channel that is activated by cold environments and menthol-like cooling compounds.  While TRPM8 is best known for its location in peripheral nerve endings, it has functio...  Read full blog post.

TRPA1: A contributor to itching and inflammation? Scratch that!
Transient receptor potential A1 (TRPA1) is an ion channel found on the plasma membrane of many cell types that functions in diverse sensory processes such as pain and temperature. The TRPA1 ion channel is specifically expressed in nociceptive neurons,...  Read full blog post.

Touch Infographic: From Touch Receptors to the Brain
The body contains thousands of receptors and nerves which allow us to experience the sense of touch, also referred to as tactile perception. The somatosensory system allows organisms to perceive and decode a wide range of tactile stimuli to allow for...  Read full blog post.

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Recent Reviews


Application: Flow
Species: Zebrafish

TinaMarie Lieu
Application: IF
Species: Human


Gene Symbol TRPA1