TRPA1 Antibody - BSA Free

Images

 
Western Blot: TRPA1 Antibody [NB110-40763] - Expression and localization of TRPA1 channels in wild-type (WT) and APP/PS1 Tg mice. Brains were harvested from WT and APP/PS1 Tg mice at 8A months old. Western blot analysis ...read more
Immunohistochemistry: TRPA1 Antibody [NB110-40763] - Staining TRPA1 in mouse intestine.
Immunocytochemistry/ Immunofluorescence: TRPA1 Antibody [NB110-40763] - Ai2 elicits TRPA1-dependent Ca2+ influx in astrocytes. Immunostaining of primary astrocytes from WT mice with anti-IgG and anti-TRPA1 antibody, ...read more
Flow Cytometry: TRPA1 Antibody [NB110-40763] - An intracellular stain was performed on A549 cells with NB110-40763C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized ...read more
Flow Cytometry: TRPA1 Antibody [NB110-40763] - An intracellular stain was performed on A549 cells with NB110-40763G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized ...read more
Western Blot: TRPA1 Antibody [NB110-40763] - The essential role of TRPA1 in Ai2-induced calcium influx in HEK293 cells. (a) Western blot analysis of TRPA1 and I+--tubulin protein levels in non-treated, vector-or ...read more
Immunocytochemistry/ Immunofluorescence: TRPA1 Antibody [NB110-40763] - Analysis of TRPA1 in HEK 293 cells (Flp In Trex system) cells.

Product Details

Summary
Reactivity Hu, Mu, Rt, Gp, ZeSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

TRPA1 Antibody - BSA Free Summary

Immunogen
A synthetic peptide made to a region within the N-terminus (residues 1-100) of the human TRPA1 protein. [Swiss-Prot# O75762]
Localization
Membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TRPA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:200
  • Immunohistochemistry 0.5-1.0 ug/ml
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:250
  • Western Blot 2 ug/ml
Application Notes
This TRPA1 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Western blot analysis. Additional modified form of TRPA1 can also be detected. Immunohistochemistry-Frozen was reported in scientific literature. Use in FLOW reported in scientific literature (PMID: 28990934). Use in ELISA reported in scientific literature (PMID: 29803505).
Theoretical MW
127.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB110-40763 in the following applications:

Publications
Read Publications using
NB110-40763 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 27748654). Zebrafish reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TRPA1 Antibody - BSA Free

  • ANKTM1
  • ANKTM1ankyrin-like with transmembrane domains 1
  • Ankyrin-like with transmembrane domains protein 1
  • Transformation-sensitive protein p120
  • transient receptor potential cation channel subfamily A member 1
  • transient receptor potential cation channel, subfamily A, member 1
  • TRPA1
  • Wasabi receptor

Background

TRPA1 (transient receptor potential cation channel subfamily A member 1; originally named as ANKTM1) is a member of transient receptor potential ion channels (TRPCs) which responds to stimuli such as cold temperatures, pungent compounds, and plays role in both nociceptor and hair cell transductions. TRPA1 is activated by variety of mechanical and chemicals stimuli including dietary irritants such as isothyocyanates (found in mustard oil, horse-radish, wasabi), allycin/garlic etc. and its activity is regulated by endogenous pro-inflammatory factors such as cyclopentane prostaglandins and byproducts of oxidative stress (e.g. 4-HNE, 4-oxononenal) which activate TRPA1 directly via covalent modification of its cysteine residues. Once TRPA1 is activated, increased intracellular Ca2+ induces the peripheral release of neuropeptides namely substance P and CGRP (calcitonin gene-related peptide), purines, and other transmitters from sensitized nerve endings, which results in neurogenic inflammation and hypersensitivity. TRPA1 is mainly expressed in small-diameter peptidergic nociceptors of the dorsal root, nodose and trigeminal ganglia, along with TRPV1, and has also been reported to be expressed in non-neuronal tissues (skeletal muscle, lung, small intestine, colon, pancreas etc) and is found dysregulated during process of carcinogenesis in certain malignancies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, Gp, Ze
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for TRPA1 Antibody (NB110-40763)(51)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Guinea Pig.

We have publications tested in 7 applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ELISA
(1)
Flow
(2)
ICC/IF
(10)
IHC
(9)
IHC-Fr
(2)
IHC-P
(3)
WB
(23)
All Applications
Filter By Species
Human
(17)
Mouse
(17)
Rat
(7)
Guinea Pig
(1)
All Species
Showing Publications 1 - 10 of 51. Show All 51 Publications.
Publications using NB110-40763 Applications Species
Chen YS, Lian YZ, Chen WC et al. Lycium barbarum Polysaccharides and Capsaicin Inhibit Oxidative Stress, Inflammatory Responses, and Pain Signaling in Rats with Dextran Sulfate Sodium-Induced Colitis International journal of molecular sciences Feb 22 2022 [PMID: 35269566] (WB, Rat) WB Rat
Kiss F, Kormos V, Szoke E et al. Functional Transient Receptor Potential Ankyrin 1 and Vanilloid 1 Ion Channels Are Overexpressed in Human Oral Squamous Cell Carcinoma International journal of molecular sciences Feb 8 2022 [PMID: 35163843] (IHC, Human) IHC Human
Yang XJ, Ling SZ, Zhou ML et al. Inhibition of TRPA1 attenuates oxidative stress-induced damage after traumatic brain injury via the ERK/AKT signaling pathway Neuroscience Feb 11 2022 [PMID: 35158017] (ICC/IF) ICC/IF
SzabO K, KemEny A, BalAzs N et al. Presence of TRPA1 Modifies CD4+/CD8+ T Lymphocyte Ratio and Activation Pharmaceuticals (Basel, Switzerland) Jan 1 2022 [PMID: 35056114] (Flow, Mouse) Flow Mouse
Chen LL, Lee MH, Chang CL et al. Suppression of Inflammatory and Fibrotic Signals by Cinnamon (Cinnamomum cassia) and Cinnamaldehyde in Cyclophosphamide-Induced Overactive Bladder in Mice Evidence-based complementary and alternative medicine : eCAM Dec 22 2021 [PMID: 34976095] (IHC-Fr, WB, Mouse) IHC-Fr, WB Mouse
Chen C, Huang X, Zhu W et al. TRPA1 triggers hyperalgesia and inflammation after tooth bleaching Scientific reports Aug 31 2021 [PMID: 34465829] (WB, IHC, Human, Rat) WB, IHC Human, Rat
Luostarinen S, Hamalainen M, Hatano N Et Al. The inflammatory regulation of TRPA1 expression in Human A549 lung epithelial cells Pulmonary pharmacology & therapeutics Jul 21 2021 [PMID: 34302984] (WB) WB
Chen L, Liu S, Xiao L et al. An initial assessment of the involvement of transglutaminase2 in eosinophilic bronchitis using a disease model developed in C57BL/6 mice Scientific reports Jun 7 2021 [PMID: 34099759] (IHC-P, Mouse) IHC-P Mouse
Huang, H, Tay, S H Et al. Targeting novel human transient receptor potential ankyrin 1 splice variation with splice-switching antisense oligonucleotides. Pain Jan 26 2021 [PMID: 33938719]
Shi L, Lin Y, Jiao Y et al. Acrolein scavenger dimercaprol offers neuroprotection in an animal model of Parkinsons disease: implication of acrolein and TRPA1 Translational Neurodegeneration Dec 1 2021 [PMID: 33910636] (IHC, Rat) IHC Rat
Show All 51 Publications.

Reviews for TRPA1 Antibody (NB110-40763) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Zebrafish.

Reviews using NB110-40763:
Filter by Applications
Flow Cytometry
(1)
Immunofluorescence
(1)
All Applications
Filter by Species
Human
(1)
Zebrafish
(1)
All Species
Images Ratings Applications Species Date Details
Flow Cytometry TRPA1 NB110-40763
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5
reviewed by:
Verified Customer
Flow Cytometry Zebrafish 10/28/2019
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Summary

ApplicationFlow Cytometry
Sample TestedZEM2S (ATCC® CRL-2147™),ZEM2S
SpeciesZebrafish
LotF-4 Dt

Comments

Comments1.     ZEM-2S cells were harvested using Tyrode/EDTA solution;
2.     Cells were fixed in 4% paraformaldehyde for 30 min at 4 oC;
3.     Cells were washed with PBS and centrifuged at 2000 g for 3 min;
4.     Approximately 10^6 cells were added to round-bottom plates and centrifuged;
5.     Blocking solution (1% BSA, 22.52 mg/ml glycine in PBS) was added to each well, and cells were kept at 4oC for 30 min;
6.     Cells were washed with PBS and incubated with rabbit TRPA1 mouse antibody (NB110-40763) in incubation solution (for each 100 mL 0.25 g of carragenine, 1 g of BSA, and 300 uL of Triton X-100). Cells were incubated overnight.
7.     Cells were washed with PBS, centrifuged, and secondary antibody (goat-anti rabbit Alexa 488 - 1:2000) was added at room temperature for 60 min;
8.     Cells were washed with PBS and harvested.
9.     Acquisition took place in FACS Canto (BD Biosciences)
Immunofluorescence TRPA1 NB110-40763
Enlarge
4
reviewed by:
Verified Customer
Immunofluorescence Human 08/21/2012
View

Summary

ApplicationImmunofluorescence
Sample TestedHEKFLPNTREX Cell line
SpeciesHuman

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TRPA1 Antibody (NB110-40763). (Showing 1 - 4 of 4 FAQs).

  1. I read the first publication (Yu S et al. 2009) referenced for NB110-40763. This product reacts with human and mouse according to your datasheet, but in that publication it was used for western blot in guinea pig. Does this mean this antibody is suitable to use for guinea pig samples?
    • Yes, according to this paper this antibody has been successfully used on guinea pig samples. This is the only testing that has been done in guinea pig though as we have done no in-house testing. It should work just fine for you.
  2. Has the specificity of NB110-40763 has been confirmed by mouse dorsal root ganglion (DRG) tissue in immunohistochemistry? Do you know what sample types have been tested?
    • We have a couple of publications with the base product catalog number NB110-40763 (which is just the unconjugated version of NB110-40763G), which have utilized our product with mouse colonic dorsal root ganglion. (PMID 19875705 and PMID 21689654). The image on our website was taken of a mouse intestine sample.
  3. What are the other two bands in the image on the datasheet? UniProt finds a theoretical MW of 127.5kDa. You find a MW of 110kDa in WB application. There are two more bands at 60kDa and 17kDa. What are these? Unspecific staining or fragments or variants of the protein? I did not find any info on variants when looking into the UniProt data (http://www.uniprot.org/uniprot/O75762).
    • We generally use gradient gels in our lab and for proteins which are multi-pass membrane protein, it is not uncommon to see an approximate 10-15% change in expected vs observed molecular weight in WB assay. The band observed at approximately 110kD is highly specific and there is no doubt that the antibody is detecting the right band. No, we have not characterized the additional low molecular weight bands yet which are considerably mild bands when compared to the expected target band. During a brief literature research this morning, I came across some papers wherein other researchers have also observed additional lower molecular weight bands and some of those references are: J Biol Chem. 2010 May 14; 285(20): 15167-15177; J Biol Chem. 2006 October 27; 281(43): 32879-32890; J Clin Invest. 2007 July 2; 117(7): 1979-1987.
  4. Human brain membrane fraction is mentioned to be used as a positive control for this antibody. Do you sell the positive control too?
    • The Western blot data that is shown on our product page for the TRPA1 antibody with catalogue number NB110-40763 was, as you have pointed out, generated from human brain membrane lysate. We do sell a human brain membrane fraction that is suitable for Western blotting, and you can see this here: TRPA1 antibody datasheet. This lysate is supplied as native protein, and we recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.

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Isotype Controls

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Blogs on TRPA1.

Winter is coming, and TRPM8 welcomes the cold!
TRPM8, or transient receptor potential melastatin 8, is a nonselective cation channel that is activated by cold environments and menthol-like cooling compounds.  While TRPM8 is best known for its location in peripheral nerve endings, it has functio...  Read full blog post.

TRPA1: A contributor to itching and inflammation? Scratch that!
Transient receptor potential A1 (TRPA1) is an ion channel found on the plasma membrane of many cell types that functions in diverse sensory processes such as pain and temperature. The TRPA1 ion channel is specifically expressed in nociceptive neurons,...  Read full blog post.

Touch Infographic: From Touch Receptors to the Brain
The body contains thousands of receptors and nerves which allow us to experience the sense of touch, also referred to as tactile perception. The somatosensory system allows organisms to perceive and decode a wide range of tactile stimuli to allow for ...  Read full blog post.

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
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Verified Customer
10/28/2019
Application: Flow Cytometry
Species: Zebrafish

Verified Customer
08/21/2012
Application: Immunofluorescence
Species: Human

Bioinformatics

Gene Symbol TRPA1
Entrez
Uniprot