TRPA1 Antibody

Images

 
Western Blot: TRPA1 Antibody [NB110-40763] - Detection of TRPA1 in human brain membrane fraction using 2ug/ml of NB110-40763.
Immunocytochemistry/ Immunofluorescence: TRPA1 Antibody [NB110-40763] - IF analysis of TRPA1 in HEK 293 cells (Flp In Trex system) cells.
Immunohistochemistry: TRPA1 Antibody [NB110-40763] - Staining TRPA1 in mouse intestine.

Product Details

Summary
Reactivity Hu, Mu, GPSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

TRPA1 Antibody Summary

Immunogen
A synthetic peptide made to a region within the N-terminus (residues 1-100) of the human TRPA1 protein. [Swiss-Prot# O75762]
Localization
Membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TRPA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:200
  • Immunohistochemistry 0.5-1.0 ug/ml
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:250
Application Notes
This TRPA1 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Western blot analysis, where a band is seen at ~110 kDa. Immunohistochemistry-Frozen was reported in scientific literature.
Reviewed Applications
Read 1 Review rated 4
using
NB110-40763 in the following applications:

Publications
Read Publications using
NB110-40763 in the following applications:

Reactivity Notes

Human, mouse and guinea pig. Immunogen sequence has 69% homology to mouse and rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TRPA1 Antibody

  • ANKTM1ankyrin-like with transmembrane domains 1
  • Ankyrin-like with transmembrane domains protein 1
  • Transformation-sensitive protein p120
  • transient receptor potential cation channel subfamily A member 1
  • transient receptor potential cation channel, subfamily A, member 1

Background

TRPA1 (transient receptor potential cation channel subfamily A member 1; originally named as ANKTM1) is a member of transient receptor potential ion channels (TRPCs) which responds to stimuli such as cold temperatures, pungent compounds, and plays role in both nociceptor and hair cell transductions. TRPA1 is activated by variety of mechanical and chemicals stimuli including dietary irritants such as isothyocyanates (found in mustard oil, horse-radish, wasabi), allycin/garlic etc. and its activity is regulated by endogenous pro-inflammatory factors such as cyclopentane prostaglandins and byproducts of oxidative stress (e.g. 4-HNE, 4-oxononenal) which activate TRPA1 directly via covalent modification of its cysteine residues. Once TRPA1 is activated, increased intracellular Ca2+ induces the peripheral release of neuropeptides namely substance P and CGRP (calcitonin gene-related peptide), purines, and other transmitters from sensitized nerve endings, which results in neurogenic inflammation and hypersensitivity. TRPA1 is mainly expressed in small-diameter peptidergic nociceptors of the dorsal root, nodose and trigeminal ganglia, along with TRPV1, and has also been reported to be expressed in non-neuronal tissues (skeletal muscle, lung, small intestine, colon, pancreas etc) and is found dysregulated during process of carcinogenesis in certain malignancies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TRPA1 Antibody (NB110-40763)(17)

We have publications tested in 3 confirmed species: Human, Mouse, Guinea Pig.

We have publications tested in 6 applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, WB.


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(1)
ICC/IF
(4)
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(4)
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(8)
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Human
(7)
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(9)
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Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using NB110-40763 Applications Species
Matsuda K, Okamoto N, Kondo M et al. Mast cell hyperactivity underpins the development of oxygen-induced retinopathy. J. Clin. Invest. 2017 Nov 01 [PMID: 28990934] (Flow, Mouse) Flow Mouse
Nummenmaa E, Hamalainen M, Moilanen LJ et al. TRPA1 expression is downregulated by dexamethasone and aurothiomalate in human chondrocytes: TRPA1 as a novel factor and drug target in arthritis RMD Open 2017 Sep 15 [PMID: 28912961] (WB, Human) WB Human
Chen JL, Ping YH, Tseng MJ et al. Notch1-promoted TRPA1 expression in erythroleukemic cells suppresses erythroid but enhances megakaryocyte differentiation. Sci Rep. 2017 Feb 21 [PMID: 28220825] (WB, Human) WB Human
Hidaka T, Ogawa E, Kobayashi EH et al. The aryl hydrocarbon receptor AhR links atopic dermatitis and air pollution via induction of the neurotrophic factor artemin. Nat. Immunol. 2016 Nov 21 [PMID: 27869817] (Mouse)

Details:
This citation used the DyLight 650 version of this antibody.
Mouse
Lee KI, Lin HC, Lee HT et al. Loss of Transient Receptor Potential Ankyrin 1 Channel Deregulates Emotion, Learning and Memory, Cognition, and Social Behavior in Mice. Mol. Neurobiol. May 19 2016 [PMID: 27194300] (WB, Mouse) WB Mouse
Lee KI, Lee HT, Lin HC et al. Role of transient receptor potential ankyrin 1 channels in Alzheimer's disease. J Neuroinflammation. Apr 28 2016 [PMID: 27121378] (WB, IHC, Mouse, Human) WB, IHC Mouse, Human
Takaya J, Mio K, Shiraishi T et al. A Potent and Site-Selective Agonist of TRPA1. J. Am. Chem. Soc. 2015 Dec 23 [PMID: 26630251] (Human) Human
Billeter AT, Galbraith N, Walker S et al. TRPA1 mediates the effects of hypothermia on the monocyte inflammatory response. Surgery 2015 Jun 5 [PMID: 26054320]
Weng HJ, Patel KN, Jeske NA et al. Tmem100 Is a Regulator of TRPA1-TRPV1 Complex and Contributes to Persistent Pain Neuron. 2015 Jan 28 [PMID: 25640077] (WB, Mouse) WB Mouse
Terada Y, Fujimura M, Nishimura S et al. Roles of Cav 3.2 and TRPA1 channels targeted by hydrogen sulfide in pancreatic nociceptive processing in mice with or without acute pancreatitis. J. Neurosci. Res. 2014 Sep 30 [PMID: 25267397] (WB, Mouse)

Details:
TRPA1 antibody used for WB in experiments involving mouse model of acute pancreatitis. Figure 5 shows TRPA1 expression in dorsal root ganglia of mice with TRPA1 knockdown achieved via repeated intrathecal administration of the mixture of two or three distinct oligodeoxynucleotides (ODNs) - AS-ODNs-TRPA1 (AS) or SC-ODNs-TRPA1 (SC). Figure 6 shows the expression levels of TRPA1 in the DRG of mice with cerulein / Cer-induced pancreatitis.
WB Mouse
Show All 17 Publications.

Review for TRPA1 Antibody (NB110-40763) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: IF.

Reviews using NB110-40763:
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IF
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Immunofluorescence TRPA1 NB110-40763
Enlarge
reviewed by:
TinaMarie Lieu
IF Human 08/21/2012
View

Summary

ApplicationImmunofluorescence
Sample TestedHEKFLPNTREX Cell line
SpeciesHuman
CommentsTRP channel antibodies are difficult to stain with specificity. This TRPA1 antibody from Novus works pretty well.

Blocking

Blocking DetailsBlock with 10% goat serum in 1% BSA & 0.1% Saponin for 2 hrs at RT

Primary Anitbody

Dilution RatioPrimary antibody of TRPA1 from Novus Biologicals – dilute in 1% BSA/0.1% Saponin (1:200 dilution)

Secondary Antibody

Secondary DescriptionSecondary antibody diluted in 1%BSA/0.1% Saponin (1:1000 dilution) – Invitrogen Alexa Flour Goat anti-rabbit
Secondary Concentration1:1000

Details

Detection NotesLeica with MetaFlour Flourescene Ratio Imaging, Pos. Control (DRG expressed TRPA1), Neg. Control (HEK nontransfected with TRPA1)
Fixation DetailsFixed in cold methanol for 2 minutes (4C)
Wash DescriptionPBS Wash 3X

Comments

CommentsTRP channel antibodies are difficult to stain with specificity. This TRPA1 antibody from Novus works pretty well.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TRPA1 Antibody (NB110-40763). (Showing 1 - 4 of 4 FAQs).

  1. I read the first publication (Yu S et al. 2009) referenced for NB110-40763. This product reacts with human and mouse according to your datasheet, but in that publication it was used for western blot in guinea pig. Does this mean this antibody is suitable to use for guinea pig samples?
    • Yes, according to this paper this antibody has been successfully used on guinea pig samples. This is the only testing that has been done in guinea pig though as we have done no in-house testing. It should work just fine for you.
  2. Has the specificity of NB110-40763 has been confirmed by mouse dorsal root ganglion (DRG) tissue in immunohistochemistry? Do you know what sample types have been tested?
    • We have a couple of publications with the base product catalog number NB110-40763 (which is just the unconjugated version of NB110-40763G), which have utilized our product with mouse colonic dorsal root ganglion. (PMID 19875705 and PMID 21689654). The image on our website was taken of a mouse intestine sample.
  3. What are the other two bands in the image on the datasheet? UniProt finds a theoretical MW of 127.5kDa. You find a MW of 110kDa in WB application. There are two more bands at 60kDa and 17kDa. What are these? Unspecific staining or fragments or variants of the protein? I did not find any info on variants when looking into the UniProt data (http://www.uniprot.org/uniprot/O75762).
    • We generally use gradient gels in our lab and for proteins which are multi-pass membrane protein, it is not uncommon to see an approximate 10-15% change in expected vs observed molecular weight in WB assay. The band observed at approximately 110kD is highly specific and there is no doubt that the antibody is detecting the right band. No, we have not characterized the additional low molecular weight bands yet which are considerably mild bands when compared to the expected target band. During a brief literature research this morning, I came across some papers wherein other researchers have also observed additional lower molecular weight bands and some of those references are: J Biol Chem. 2010 May 14; 285(20): 15167-15177; J Biol Chem. 2006 October 27; 281(43): 32879-32890; J Clin Invest. 2007 July 2; 117(7): 1979-1987.
  4. Human brain membrane fraction is mentioned to be used as a positive control for this antibody. Do you sell the positive control too?
    • The Western blot data that is shown on our product page for the TRPA1 antibody with catalogue number NB110-40763 was, as you have pointed out, generated from human brain membrane lysate. We do sell a human brain membrane fraction that is suitable for Western blotting, and you can see this here: TRPA1 antibody datasheet. This lysate is supplied as native protein, and we recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-40763AF405
Alexa Fluor 488 Labeled NB110-40763AF488
Alexa Fluor 647 Labeled NB110-40763AF647
Alexa Fluor 700 Labeled NB110-40763AF700
Biotin Labeled NB110-40763B
DyLight 405 Labeled NB110-40763V
DyLight 488 Labeled NB110-40763G
DyLight 550 Labeled NB110-40763R
DyLight 650 Labeled NB110-40763C
FITC Labeled NB110-40763F
HRP Labeled NB110-40763H

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Blogs on TRPA1.

Winter is coming, and TRPM8 welcomes the cold!
TRPM8, or transient receptor potential melastatin 8, is a nonselective cation channel that is activated by cold environments and menthol-like cooling compounds.  While TRPM8 is best known for its location in peripheral nerve endings, it has functio...  Read full blog post.

TRPA1: A contributor to itching and inflammation? Scratch that!
Transient receptor potential A1 (TRPA1) is an ion channel found on the plasma membrane of many cell types that functions in diverse sensory processes such as pain and temperature. The TRPA1 ion channel is specifically expressed in nociceptive neurons,...  Read full blog post.

Touch Infographic: From Touch Receptors to the Brain
The body contains thousands of receptors and nerves which allow us to experience the sense of touch, also referred to as tactile perception. The somatosensory system allows organisms to perceive and decode a wide range of tactile stimuli to allow for...  Read full blog post.

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TinaMarie Lieu
08/21/2012
Application: IF
Species: Human

Bioinformatics

Gene Symbol TRPA1
Entrez
Uniprot