TRPA1 Antibody - BSA Free

Images

 
Western Blot: TRPA1 Antibody [NB110-40763] - Expression and localization of TRPA1 channels in wild-type (WT) and APP/PS1 Tg mice. Brains were harvested from WT and APP/PS1 Tg mice at 8A months old. Western blot analysis ...read more
Immunohistochemistry: TRPA1 Antibody [NB110-40763] - Staining TRPA1 in mouse intestine.
Immunocytochemistry/ Immunofluorescence: TRPA1 Antibody [NB110-40763] - Ai2 elicits TRPA1-dependent Ca2+ influx in astrocytes. Immunostaining of primary astrocytes from WT mice with anti-IgG and anti-TRPA1 antibody, ...read more
Flow Cytometry: TRPA1 Antibody [NB110-40763] - An intracellular stain was performed on A549 cells with NB110-40763C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized ...read more
Flow Cytometry: TRPA1 Antibody [NB110-40763] - An intracellular stain was performed on A549 cells with NB110-40763G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized ...read more
Western Blot: TRPA1 Antibody [NB110-40763] - The essential role of TRPA1 in Ai2-induced calcium influx in HEK293 cells. (a) Western blot analysis of TRPA1 and I+--tubulin protein levels in non-treated, vector-or ...read more
Immunocytochemistry/ Immunofluorescence: TRPA1 Antibody [NB110-40763] - Analysis of TRPA1 in HEK 293 cells (Flp In Trex system) cells.
Evaluation of anti-TRPA1 antibody specificity by western blotting and immunofluorescence. b) The performance of the same antibodies was evaluated by immunofluorescence (red) in comparison to the expression of the GFP ...read more
Oxygen-induced degranulation of mast cells was induced by TRPA1.(B and C) To identify O2-sensing molecules on mast cells, TRPA1 was detected by flow cytometry and immunoblotting. Results are representative of 3 ...read more
The production of DSBs depends on Top1 degradation in CPT-treated quiescent cells. (A–C) Serum-starved WI38 hTERT cells were co-transfected with siRNAs against cullin 3 and cullin 4B or against a control sequence and ...read more
dsRNA-induced SGs require G3BP1 and G3BP2, whereas RLBs do not.A, immunoblot for G3BP1, RNase L, GAPDH in parental (WT), RL-KO, G3BP-KO, and RL/G3BP-KO U-2 OS cells. B, immunoblot for G3BP1 and G3BP2 in RL-KO and ...read more
SOD2 mRNA and protein expression. RT-PCR: (A) SOD2 mRNA expression is higher in adults, and highest in the adult parenchyma. (B) No exposure effects on SOD2 mRNA were observed in neonates. (C) Adult SOD2 mRNA was ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Gp, ZeSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

TRPA1 Antibody - BSA Free Summary

Immunogen
A synthetic peptide made to a region within the N-terminus (residues 1-100) of the human TRPA1 protein. [Swiss-Prot# O75762]
Localization
Membrane
Specificity
Additional modified form of TRPA1 can also be detected.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TRPA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA reported in scientific literature (PMID 29803505)
  • Flow Cytometry reported in scientific literature (PMID 28990934)
  • Immunocytochemistry/ Immunofluorescence 1:200
  • Immunohistochemistry 0.5-1.0 ug/ml
  • Immunohistochemistry-Frozen reported in scientific literature
  • Immunohistochemistry-Paraffin 1:100-1:250
  • Western Blot 2 ug/ml
Theoretical MW
127.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB110-40763 in the following applications:

Publications
Read Publications using
NB110-40763 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 27748654). Zebrafish reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TRPA1 Antibody - BSA Free

  • ANKTM1
  • ANKTM1ankyrin-like with transmembrane domains 1
  • Ankyrin-like with transmembrane domains protein 1
  • Transformation-sensitive protein p120
  • transient receptor potential cation channel subfamily A member 1
  • transient receptor potential cation channel, subfamily A, member 1
  • TRPA1
  • Wasabi receptor

Background

TRPA1 (transient receptor potential cation channel subfamily A member 1; originally named as ANKTM1) is a member of transient receptor potential ion channels (TRPCs) which responds to stimuli such as cold temperatures, pungent compounds, and plays role in both nociceptor and hair cell transductions. TRPA1 is activated by variety of mechanical and chemicals stimuli including dietary irritants such as isothyocyanates (found in mustard oil, horse-radish, wasabi), allycin/garlic etc. and its activity is regulated by endogenous pro-inflammatory factors such as cyclopentane prostaglandins and byproducts of oxidative stress (e.g. 4-HNE, 4-oxononenal) which activate TRPA1 directly via covalent modification of its cysteine residues. Once TRPA1 is activated, increased intracellular Ca2+ induces the peripheral release of neuropeptides namely substance P and CGRP (calcitonin gene-related peptide), purines, and other transmitters from sensitized nerve endings, which results in neurogenic inflammation and hypersensitivity. TRPA1 is mainly expressed in small-diameter peptidergic nociceptors of the dorsal root, nodose and trigeminal ganglia, along with TRPV1, and has also been reported to be expressed in non-neuronal tissues (skeletal muscle, lung, small intestine, colon, pancreas etc) and is found dysregulated during process of carcinogenesis in certain malignancies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRPA1 Antibody (NB110-40763)(71)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Guinea Pig.

We have publications tested in 10 applications: ELISA, FLOW, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, IP, In Vivo, WB.


Filter By Application
ELISA
(1)
FLOW
(2)
ICC/IF
(14)
IF/IHC
(10)
IHC
(1)
IHC-Fr
(3)
IHC-P
(4)
IP
(1)
In Vivo
(1)
WB
(29)
All Applications
Filter By Species
Human
(21)
Mouse
(20)
Rat
(8)
Guinea Pig
(1)
All Species
Showing Publications 1 - 10 of 71. Show All 71 Publications.
Publications using NB110-40763 Applications Species
Warfield R, Robinson JA, Podgorski RM et al. Neuroinflammation in the Dorsal Root Ganglia and Dorsal Horn Contributes to Persistence of Nociceptor Sensitization in SIV-Infected Antiretroviral Therapy-Treated Macaques The American journal of pathology 2023-09-19 [PMID: 37734588] (IHC) IHC
Luostarinen S, Hämäläinen M, Pemmari A, Moilanen E The regulation of TRPA1 expression and function by Th1 and Th2-type inflammation in human A549 lung epithelial cells Inflammation research : official journal of the European Histamine Research Society ... [et al.] 2023-06-29 [PMID: 37386145] (WB, Human) WB Human
Wang M, Zhao M, Xu S et al. TRPA1 deficiency attenuates cardiac fibrosis via regulating GRK5/NFAT signaling in diabetic rats Biochemical pharmacology 2023-06-26 [PMID: 37380112] (IHC-P, Rat) IHC-P Rat
Pandey M, Zhang JH, Adikaram PR et al. Specific regulation of mechanical nociception by G?5 involves GABA-B receptors JCI insight 2023-05-23 [PMID: 37219953] (IHC-Fr, Mouse)

Details:
Dilutions: 1:200
IHC-Fr Mouse
Pereira GC, Piton E, Bornholdt J et al. TRPA1 participation in behavioral impairment induced by chronic corticosterone administration Psychopharmacology 2022-12-15 [PMID: 36520197]
Yang N, Shao H, Deng J et al. Dictamnine ameliorates chronic itch in DNFB-induced atopic dermatitis mice via inhibiting MrgprA3 Biochemical pharmacology 2022-12-06 [PMID: 36493846] (WB, Mouse)

Details:
Dilution used 1:1000
WB Mouse
Ma S, Wang DH. Knockout of Trpa1 accelerates age-related cardiac fibrosis and dysfunction PLOS ONE 2022-09-14 [PMID: 36103570] (WB) WB
S�nchez JC, Mu�oz LV, Galindo-M�rquez ML et al. Paclitaxel Regulates TRPA1 Function and Expression Through PKA and PKC Neurochemical Research 2023-01-01 [PMID: 36098890] (WB) WB
Kusiak AA, Jakubowska MA, Stopa KB et al. Activation of pancreatic stellate cells attenuates intracellular Ca2+ signals due to downregulation of TRPA1 and protects against cell death induced by alcohol metabolites Cell death & disease 2022-08-29 [PMID: 36038551] (ICC/IF, Human)

Details:
Dilutions: 1:300
ICC/IF Human
Vasavda C, Xu R, Liew J et al. Identification of the NRF2 transcriptional network as a therapeutic target for trigeminal neuropathic pain Science advances 2022-08-05 [PMID: 35921423] (WB, Mouse)

Details:
Dilutions: 1:1000; Supplementary Figure 2
WB Mouse
Show All 71 Publications.

Reviews for TRPA1 Antibody (NB110-40763) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Zebrafish.

Reviews using NB110-40763:
Filter by Applications
Flow
(1)
IF
(1)
All Applications
Filter by Species
Human
(1)
Zebrafish
(1)
All Species
Images Ratings Applications Species Date Details
Flow Cytometry TRPA1 NB110-40763
Enlarge
5
reviewed by:
LEONARDO DE ASSIS
Flow Zebrafish 10/28/2019
View

Summary

ApplicationFlow Cytometry
Sample TestedZEM2S (ATCC® CRL-2147™),ZEM2S
SpeciesZebrafish
LotF-4 Dt

Comments

Comments1.     ZEM-2S cells were harvested using Tyrode/EDTA solution;
2.     Cells were fixed in 4% paraformaldehyde for 30 min at 4 oC;
3.     Cells were washed with PBS and centrifuged at 2000 g for 3 min;
4.     Approximately 10^6 cells were added to round-bottom plates and centrifuged;
5.     Blocking solution (1% BSA, 22.52 mg/ml glycine in PBS) was added to each well, and cells were kept at 4oC for 30 min;
6.     Cells were washed with PBS and incubated with rabbit TRPA1 mouse antibody (NB110-40763) in incubation solution (for each 100 mL 0.25 g of carragenine, 1 g of BSA, and 300 uL of Triton X-100). Cells were incubated overnight.
7.     Cells were washed with PBS, centrifuged, and secondary antibody (goat-anti rabbit Alexa 488 - 1:2000) was added at room temperature for 60 min;
8.     Cells were washed with PBS and harvested.
9.     Acquisition took place in FACS Canto (BD Biosciences)
Immunofluorescence TRPA1 NB110-40763
Enlarge
4
reviewed by:
TinaMarie Lieu
IF Human 08/21/2012
View

Summary

ApplicationImmunofluorescence
Sample TestedHEKFLPNTREX Cell line
SpeciesHuman

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TRPA1 Antibody (NB110-40763). (Showing 1 - 4 of 4 FAQs).

  1. I read the first publication (Yu S et al. 2009) referenced for NB110-40763. This product reacts with human and mouse according to your datasheet, but in that publication it was used for western blot in guinea pig. Does this mean this antibody is suitable to use for guinea pig samples?
    • Yes, according to this paper this antibody has been successfully used on guinea pig samples. This is the only testing that has been done in guinea pig though as we have done no in-house testing. It should work just fine for you.
  2. Has the specificity of NB110-40763 has been confirmed by mouse dorsal root ganglion (DRG) tissue in immunohistochemistry? Do you know what sample types have been tested?
    • We have a couple of publications with the base product catalog number NB110-40763 (which is just the unconjugated version of NB110-40763G), which have utilized our product with mouse colonic dorsal root ganglion. (PMID 19875705 and PMID 21689654). The image on our website was taken of a mouse intestine sample.
  3. What are the other two bands in the image on the datasheet? UniProt finds a theoretical MW of 127.5kDa. You find a MW of 110kDa in WB application. There are two more bands at 60kDa and 17kDa. What are these? Unspecific staining or fragments or variants of the protein? I did not find any info on variants when looking into the UniProt data (http://www.uniprot.org/uniprot/O75762).
    • We generally use gradient gels in our lab and for proteins which are multi-pass membrane protein, it is not uncommon to see an approximate 10-15% change in expected vs observed molecular weight in WB assay. The band observed at approximately 110kD is highly specific and there is no doubt that the antibody is detecting the right band. No, we have not characterized the additional low molecular weight bands yet which are considerably mild bands when compared to the expected target band. During a brief literature research this morning, I came across some papers wherein other researchers have also observed additional lower molecular weight bands and some of those references are: J Biol Chem. 2010 May 14; 285(20): 15167-15177; J Biol Chem. 2006 October 27; 281(43): 32879-32890; J Clin Invest. 2007 July 2; 117(7): 1979-1987.
  4. Human brain membrane fraction is mentioned to be used as a positive control for this antibody. Do you sell the positive control too?
    • The Western blot data that is shown on our product page for the TRPA1 antibody with catalogue number NB110-40763 was, as you have pointed out, generated from human brain membrane lysate. We do sell a human brain membrane fraction that is suitable for Western blotting, and you can see this here: TRPA1 antibody datasheet. This lysate is supplied as native protein, and we recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.

Secondary Antibodies

 

Isotype Controls

Additional TRPA1 Products

Research Areas for TRPA1 Antibody (NB110-40763)

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Blogs on TRPA1.

Winter is coming, and TRPM8 welcomes the cold!
TRPM8, or transient receptor potential melastatin 8, is a nonselective cation channel that is activated by cold environments and menthol-like cooling compounds.  While TRPM8 is best known for its location in peripheral nerve endings, it has functio...  Read full blog post.

TRPA1: A contributor to itching and inflammation? Scratch that!
Transient receptor potential A1 (TRPA1) is an ion channel found on the plasma membrane of many cell types that functions in diverse sensory processes such as pain and temperature. The TRPA1 ion channel is specifically expressed in nociceptive neurons,...  Read full blog post.

Touch Infographic: From Touch Receptors to the Brain
The body contains thousands of receptors and nerves which allow us to experience the sense of touch, also referred to as tactile perception. The somatosensory system allows organisms to perceive and decode a wide range of tactile stimuli to allow for ...  Read full blog post.

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

LEONARDO DE ASSIS
10/28/2019
Application: Flow
Species: Zebrafish

TinaMarie Lieu
08/21/2012
Application: IF
Species: Human

Bioinformatics

Gene Symbol TRPA1
Entrez
Uniprot