TRPA1 Antibody


Western Blot: TRPA1 Antibody [NB110-40763] - Detection of TRPA1 in human brain membrane fraction using 2ug/ml of NB110-40763.
Immunocytochemistry/ Immunofluorescence: TRPA1 Antibody [NB110-40763] - IF analysis of TRPA1 in HEK 293 cells (Flp In Trex system) cells.
Immunohistochemistry: TRPA1 Antibody [NB110-40763] - Staining TRPA1 in mouse intestine.

Product Details

Reactivity Hu, Mu, GPSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
1.0 mg/ml

TRPA1 Antibody Summary

A synthetic peptide made to a region within the N-terminus (residues 1-100) of the human TRPA1 protein. [Swiss-Prot# O75762]
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
1X PBS, 0.05% NaN3
0.05% Sodium Azide
1.0 mg/ml
Immunogen affinity purified


Application Notes
This TRPA1 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Western blot analysis, where a band is seen at ~110 kDa. Immunohistochemistry-Frozen was reported in scientific literature.
Reviewed Applications
Read 1 Review rated 4
NB110-40763 in the following application:

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NB110-40763 in the following applications:

Reactivity Notes

Human, mouse and guinea pig. Immunogen sequence has 69% homology to mouse and rat.

Alternate Names for TRPA1 Antibody

  • ANKTM1ankyrin-like with transmembrane domains 1
  • Ankyrin-like with transmembrane domains protein 1
  • Transformation-sensitive protein p120
  • transient receptor potential cation channel subfamily A member 1
  • transient receptor potential cation channel, subfamily A, member 1


TRPA1 (transient receptor potential cation channel subfamily A member 1; originally named as ANKTM1) is a member of transient receptor potential ion channels (TRPCs) which responds to stimuli such as cold temperatures, pungent compounds, and plays role in both nociceptor and hair cell transductions. TRPA1 is activated by variety of mechanical and chemicals stimuli including dietary irritants such as isothyocyanates (found in mustard oil, horse-radish, wasabi), allycin/garlic etc. and its activity is regulated by endogenous pro-inflammatory factors such as cyclopentane prostaglandins and byproducts of oxidative stress (e.g. 4-HNE, 4-oxononenal) which activate TRPA1 directly via covalent modification of its cysteine residues. Once TRPA1 is activated, increased intracellular Ca2+ induces the peripheral release of neuropeptides namely substance P and CGRP (calcitonin gene-related peptide), purines, and other transmitters from sensitized nerve endings, which results in neurogenic inflammation and hypersensitivity. TRPA1 is mainly expressed in small-diameter peptidergic nociceptors of the dorsal root, nodose and trigeminal ganglia, along with TRPV1, and has also been reported to be expressed in non-neuronal tissues (skeletal muscle, lung, small intestine, colon, pancreas etc) and is found dysregulated during process of carcinogenesis in certain malignancies.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TRPA1 Antibody (NB110-40763)(13)

We have publications tested in 3 confirmed species: Human, Mouse, Guinea Pig.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB.

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Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NB110-40763 Applications Species
Lee KI, Lin HC, Lee HT et al. Loss of Transient Receptor Potential Ankyrin 1 Channel Deregulates Emotion, Learning and Memory, Cognition, and Social Behavior in Mice. Mol. Neurobiol. May 19 2016 12:00AM [PMID:27194300] (WB, Mouse) WB Mouse
Lee KI, Lee HT, Lin HC et al. Role of transient receptor potential ankyrin 1 channels in Alzheimer's disease. J Neuroinflammation. Apr 28 2016 12:00AM [PMID:27121378] (WB, IHC, Mouse, Human) WB, IHC Mouse, Human
Takaya J, Mio K, Shiraishi T et al. A Potent and Site-Selective Agonist of TRPA1. J. Am. Chem. Soc. 2015 Dec 23 [PMID:26630251] (Human) Human
Billeter AT, Galbraith N, Walker S et al. TRPA1 mediates the effects of hypothermia on the monocyte inflammatory response. Surgery 2015 Jun 5 [PMID:26054320]
Weng HJ, Patel KN, Jeske NA et al. Tmem100 Is a Regulator of TRPA1-TRPV1 Complex and Contributes to Persistent Pain Neuron. 2015 Jan 28 [PMID:25640077] (WB, Mouse) WB Mouse
Terada Y, Fujimura M, Nishimura S et al. Roles of Cav 3.2 and TRPA1 channels targeted by hydrogen sulfide in pancreatic nociceptive processing in mice with or without acute pancreatitis. J. Neurosci. Res. 2014 Sep 30 [PMID:25267397] (WB, Mouse) WB Mouse
Fusi C, Materazzi S, Minocci D et al. Transient Receptor Potential Vanilloid 4 (TRPV4) Is Downregulated in Keratinocytes in Human Non-Melanoma Skin Cancer. J. Invest. Dermatol. 2014 Mar 18 [PMID:24643128] (IHC-P, Human) IHC-P Human
Oh MH, Oh SY, Lu J et al. TRPA1-Dependent Pruritus in IL-13-Induced Chronic Atopic Dermatitis. J Immunol. 2013 Dec 1 [PMID:24140646] (IHC, ICC/IF, Mouse, Human) IHC, ICC/IF Mouse, Human
Okubo K, Matsumura M, Kawaishi Y et al. Hydrogen sulfide-induced mechanical hyperalgesia and allodynia require activation of both Cav3 2 and TRPA1 channels in mice. Br J Pharmacol 2012 Jul [PMID:22300342]
Mukhopadhyay I, Gomes P, Aranake S, Shetty M, Karnik P, Damle M, Kuruganti S, Thorat S, Khairatkar-Joshi N. Expression of functional TRPA1 receptor on human lung fibroblast and epithelial cells. J Recept Signal Transduct Res;31(5):350-8. 2011 Oct. [PMID:21848366] (WB, Human) WB Human
Show All 13 Publications.

Reviews for TRPA1 Antibody (NB110-40763) (1) 41

Average Rating: 4
(Based on 1 reviews)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: IF.

Reviews using NB110-40763:
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Images Ratings Applications Species Date Details
Immunofluorescence TRPA1 NB110-40763
reviewed by:
IF Human 08/21/2012


Sample TestedHEKFLPNTREX Cell line
CommentsTRP channel antibodies are difficult to stain with specificity. This TRPA1 antibody from Novus works pretty well.


Blocking DetailsBlock with 10% goat serum in 1% BSA & 0.1% Saponin for 2 hrs at RT

Primary Anitbody

Dilution RatioPrimary antibody of TRPA1 from Novus Biologicals – dilute in 1% BSA/0.1% Saponin (1:200 dilution)

Secondary Antibody

Secondary DescriptionSecondary antibody diluted in 1%BSA/0.1% Saponin (1:1000 dilution) – Invitrogen Alexa Flour Goat anti-rabbit
Secondary Concentration1:1000


Detection NotesLeica with MetaFlour Flourescene Ratio Imaging, Pos. Control (DRG expressed TRPA1), Neg. Control (HEK nontransfected with TRPA1)
Fixation DetailsFixed in cold methanol for 2 minutes (4C)
Wash DescriptionPBS Wash 3X


CommentsTRP channel antibodies are difficult to stain with specificity. This TRPA1 antibody from Novus works pretty well.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TRPA1 Antibody (NB110-40763). (Showing 1 - 4 of 4 FAQs).

  1. I read the first publication (Yu S et al. 2009) referenced for NB110-40763. This product reacts with human and mouse according to your datasheet, but in that publication it was used for western blot in guinea pig. Does this mean this antibody is suitable to use for guinea pig samples?
    • Yes, according to this paper this antibody has been successfully used on guinea pig samples. This is the only testing that has been done in guinea pig though as we have done no in-house testing. It should work just fine for you.
  2. Has the specificity of NB110-40763 has been confirmed by mouse dorsal root ganglion (DRG) tissue in immunohistochemistry? Do you know what sample types have been tested?
    • We have a couple of publications with the base product catalog number NB110-40763 (which is just the unconjugated version of NB110-40763G), which have utilized our product with mouse colonic dorsal root ganglion. (PMID 19875705 and PMID 21689654). The image on our website was taken of a mouse intestine sample.
  3. What are the other two bands in the image on the datasheet? UniProt finds a theoretical MW of 127.5kDa. You find a MW of 110kDa in WB application. There are two more bands at 60kDa and 17kDa. What are these? Unspecific staining or fragments or variants of the protein? I did not find any info on variants when looking into the UniProt data (
    • We generally use gradient gels in our lab and for proteins which are multi-pass membrane protein, it is not uncommon to see an approximate 10-15% change in expected vs observed molecular weight in WB assay. The band observed at approximately 110kD is highly specific and there is no doubt that the antibody is detecting the right band. No, we have not characterized the additional low molecular weight bands yet which are considerably mild bands when compared to the expected target band. During a brief literature research this morning, I came across some papers wherein other researchers have also observed additional lower molecular weight bands and some of those references are: J Biol Chem. 2010 May 14; 285(20): 15167-15177; J Biol Chem. 2006 October 27; 281(43): 32879-32890; J Clin Invest. 2007 July 2; 117(7): 1979-1987.
  4. Human brain membrane fraction is mentioned to be used as a positive control for this antibody. Do you sell the positive control too?
    • The Western blot data that is shown on our product page for the TRPA1 antibody with catalogue number NB110-40763 was, as you have pointed out, generated from human brain membrane lysate. We do sell a human brain membrane fraction that is suitable for Western blotting, and you can see this here: TRPA1 antibody datasheet. This lysate is supplied as native protein, and we recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB110-40763AF405
Alexa Fluor (R) 488 Labeled NB110-40763AF488
Alexa Fluor (R) 647 Labeled NB110-40763AF647
Alexa Fluor (R) 700 Labeled NB110-40763AF700
Biotin Labeled NB110-40763B
DyLight 405 Labeled NB110-40763V
DyLight 488 Labeled NB110-40763G
DyLight 550 Labeled NB110-40763R
DyLight 650 Labeled NB110-40763C
FITC Labeled NB110-40763F
HRP Labeled NB110-40763H

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Blogs on TRPA1.

TRPA1: A contributor to itching and inflammation? Scratch that!
Transient receptor potential A1 (TRPA1) is an ion channel found on the plasma membrane of many cell types that functions in diverse sensory processes such as pain and temperature. The TRPA1 ion channel is specifically expressed in nociceptive neurons,...  Read full blog post.

Touch Infographic: From Touch Receptors to the Brain
The body contains thousands of receptors and nerves which allow us to experience the sense of touch, also referred to as tactile perception. The somatosensory system allows organisms to perceive and decode a wide range of tactile stimuli to allow for...  Read full blog post.

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Product PDFs


Gene Symbol TRPA1

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