TRPM4 Antibody (CL11214) - BSA Free

Immunohistochemistry-Paraffin: TRPM4 Antibody (CL11214) [NBP3-07990] - Analysis in human duodenum and pancreas tissues using NBP3-07990 antibody. Corresponding TRPM4 RNA-seq data are presented for the same tissues.

Western Blot: TRPM4 Antibody (CL11214) [NBP3-07990] - Analysis in human cell line RT-4.

Immunocytochemistry/Immunofluorescence: TRPM4 Antibody (CL11214) [NBP3-07990] - Staining of MCF7 cells using the Anti-TRPM4 monoclonal antibody, showing specific staining in the plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Immunohistochemistry-Paraffin: TRPM4 Antibody (CL11214) [NBP3-07990] - Staining of human prostate shows moderate membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: TRPM4 Antibody (CL11214) [NBP3-07990] - Staining of human duodenum shows strong membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: TRPM4 Antibody (CL11214) [NBP3-07990] - Staining of human kidney shows moderate to strong membranous positivity in cells in tubules.

Immunohistochemistry-Paraffin: TRPM4 Antibody (CL11214) [NBP3-07990] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: CL11214
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

TRPM4 Antibody (CL11214) - BSA Free Summary

• Description: Novus Biologicals Mouse TRPM4 Antibody (CL11214) - BSA Free (NBP3-07990) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a recombinant protein derived from Q8TD43, with the exact immunogen sequence remaining proprietary.
• Predicted Species: Mouse (93%), Rat (91%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TRPM4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1 ug/ml
• Application Notes: ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for TRPM4 Antibody (CL11214) - BSA Free

• Calcium-activated non-selective cation channel 1
• FLJ20041
• hTRPM4
• Long transient receptor potential channel 4
• LTrpC4
• LTrpC-4
• Melastatin-4
• PFHB1B
• transient receptor potential cation channel subfamily M member 4
• transient receptor potential cation channel, subfamily M, member 4
• TRPM4
• TRPM4B

Background

FUNCTION: Calcium-activated non selective (CAN) cation channel that mediates membrane depolarization. While it is activated by increase in intracellular Ca(2+), it is impermeable to it. Mediates transport of monovalent cations (Na(+) > K(+) > Cs(+) > Li(+)), leading to depolarize the membrane. It thereby plays a central role in cadiomyocytes, neurons from entorhinal cortex, dorsal root and vomeronasal neurons, endocrine pancreas cells, kidney epithelial cells, cochlea hair cells etc. Participates in T-cell activation by modulating Ca(2+) oscillations after T lymphocyte activation, which is required for NFAT-dependent IL2 production. Involved in myogenic constriction of cerebral arteries. Controls insulin secretion in pancreatic beta-cells. May also be involved in pacemaking or could cause irregular electrical activity under conditions of Ca(2+) overload.; ENZYME REGULATION: Gating is voltage-dependent and repressed by decavanadate. Calmodulin-binding confers the Ca(2+) sensitivity. ATP is able to restore Ca(2+) sensitivity after desensitization. Phosphatidylinositol 4,5-biphosphate (PIP2)-binding strongly enhances activity, by increasing the channel's Ca(2+) sensitivity and shifting its voltage dependence of activation towards negative potentials. Activity is also enhanced by 3,5-bis(trifluoromethyl)pyrazole derivative (BTP2). SUBUNIT: Homomultimer. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Widely expressed with a high expression in intestine and prostate. In brain, it is both expressed in whole cerebral arteries and isolated vascular smooth muscle cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRPM4