TRPM2 Antibody - BSA Free

Immunohistochemistry-Paraffin: TRPM2 Antibody [NBP1-33416] - Paraffin-embedded rat liver. TRPM2 antibody [N1N2-2], N-term diluted at 1:500.

Immunohistochemistry-Paraffin: TRPM2 Antibody [NBP1-33416] - Paraffin-embedded MDAMB468 xenograft, using antibody at 1:100 dilution.

Western Blot: TRPM2 Antibody [NBP1-33416] - HepG2 whole cell and membrane extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TRPM2 antibody [N1N2-2], N-term (NBP1-33416) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

HepG2 whole cell and membrane extracts (30 ug) were separated by 5% SDS-PAGE, and the membranes were blotted with TRPM2 antibody [N1N2-2], N-term (NBP1-33416) diluted at 1:500 and competitor's antibody (ACC-043) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated with Novus and does not endorse this product.

• Reactivity: Hu, Rt, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TRPM2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TRPM2 Antibody - BSA Free (NBP1-33416) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human TRPM2. The exact sequence is proprietary.
• Localization: Membrane, Multi-pass membrane protein
• Predicted Species: Mouse (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRPM2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 171 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for TRPM2 Antibody - BSA Free

• EC 3.6.1.13
• EREG1MGC133383
• Estrogen-responsive element-associated gene 1 protein
• KNP3LTrpC-2
• Long transient receptor potential channel 2
• LTrpC2
• LTRPC2TRPC7transient receptor potential cation channel subfamily M member 2
• NUDT9H
• NUDT9L1
• transient receptor potential cation channel, subfamily M, member 2
• Transient receptor potential channel 7
• TrpC7

Background

TRPM2 belongs to the ion transport protein family. This family contains Sodium, Potassium, Calcium and ion channels. This family has 6 transmembrane helices in which the last two helices flank a loop which determines ion selectivity. TRPM2 is activated by oxidative stress and confers susceptibility to cell death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRPM2