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TRPC2 Antibody

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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TRPC2 Antibody Summary

Immunogen
A synthetic peptide from mouse TRPC2 conjugated to blue carrier protein was used as the antigen.
Clonality
Polyclonal
Host
Rabbit
Gene
TRPC2
Purity
Unpurified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Paraffin 1:1000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Lyophilized from whole serum
Preservative
No Preservative
Purity
Unpurified

Alternate Names for TRPC2 Antibody

  • transient receptor potential cation channel, subfamily C, member 2, pseudogene

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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TYRP1 Antibody
NBP1-88370
Western Blot: TYRP1 Antibody [NBP1-88370] - Analysis in control (vector only transfected HEK293T lysate) and TYRP1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/Immunofluorescence: TYRP1 Antibody [NBP1-88370] - Staining of human cell line SK-MEL-30 shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: TYRP1 Antibody [NBP1-88370] - Staining of human cerebral cortex shows no positivity in neurons as expected.
(2 Publications)
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
TRPM2 Antibody
NB110-81601
Western Blot: TRPM2 Antibody [NB110-81601] - Lane 1: CHO cells untransfected. Lane 2: CHO cell transfected with rat TRPM2 Lane 3: Mouse cortical neurons (cultured) Photo courtesy of Dr. Paco Herson, Oregon Health & Science University.
Immunocytochemistry/Immunofluorescence: TRPM2 Antibody [NB110-81601] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-TRPM2 at 5 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunocytochemistry/Immunofluorescence: TRPM2 Antibody [NB110-81601] - Staining of rat hippocampus using NB110-81601. TRPM2 is stained red, GFAP is stained green and nuclei staining is blue. Photo courtesy of Dr. Ji-Zhong Bai, The University of Auckland, New Zealand.
(8 Publications)
Species: Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr
TRPC1 Antibody
NB100-98844
Immunocytochemistry/Immunofluorescence: TRPC1 Antibody [NB100-98844] - IF (confocal) on rat trigeminal at 1 : 500 dilution using Rabbit antibody to 2d extracellular loop of TRPC1: whole serum, DAPI counterstained appearing in blue.
Immunocytochemistry/Immunofluorescence: TRPC1 Antibody [NB100-98844] - IF (confocal) on rat brain at 1:500 dilution using Rabbit antibody to 2d extracellular loop of TRPC1: whole serum, DAPI counterstained appearing in blue.
Immunocytochemistry/Immunofluorescence: TRPC1 Antibody [NB100-98844] - IF (confocal) on rat olfactory bulb at 1:500 dilution using Rabbit antibody to TRPC1: whole serum, DAPI counterstained appearing in blue.
(2 Publications)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC
TBP like protein
TLP Antibody
NBP1-31133
Western Blot: TBP like protein TLP Antibody [NBP1-31133] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE, antibody diluted at 1:2000.
Immunocytochemistry/Immunofluorescence: TBP like protein TLP Antibody [NBP1-31133] - NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TLP protein stained by TLP antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunohistochemistry-Paraffin: TBP like protein TLP Antibody [NBP1-31133] - Paraffin-embedded mouse lung. TLP antibody dilution: 1:500.
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
TRPC3 Antibody
NBP1-77262
Western Blot: TRPC3 Antibody [NBP1-77262] - Human brain tissue lysate. (A) 1 and (B) 2 ug/mL.
Immunohistochemistry-Paraffin: TRPC3 Antibody [NBP1-77262] - Mouse heart tissue with TRPC3 antibody at 2 ug/mL.
Immunofluorescence: TRPC3 Antibody [NBP1-77262] - Mouse Heart cells with TRPC3 antibody at 20 ug/mL.
Species: Hu, Mu
Applications: WB, ELISA, IHC, IHC-P, IF
TRPC6 Antibody
NBP1-00142
Western Blot: TRPC6 Antibody [NBP1-00142] - Analysis of TRPC6 in human placenta lysate (35ug protein in RIPA buffer) using this antibody at 0.03 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
(1 Review)
(4 Publications)
Species: Hu, Mu
Applications: WB, IP, PEP-ELISA
TRPC4 Antibody
NBP1-20989
Western Blot: TRPC4 Antibody [NBP1-20989] - Staining of Rat Brain lysate (35 ug protein in RIPA buffer) with antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: TRPC4 Antibody [NBP1-20989] - Staining of paraffin embedded Human Placenta with antibody at 4.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: TRPC4 Antibody [NBP1-20989] - Staining of paraffin embedded Human Kidney with antibody at 4.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
(2 Publications)
Species: Hu, Rt
Applications: WB, IHC, IHC-P, PEP-ELISA
TRP2 Antibody
NBP1-86892
Western Blot: TRP2 Antibody [NBP1-86892] - Analysis in human cell line SK-MEL-30 and human cell line U-2 OS.
Immunohistochemistry-Paraffin: TRP2 Antibody [NBP1-86892] - Staining of human skin.
Immunohistochemistry-Paraffin: TRP2 Antibody [NBP1-86892] - Staining of human skin shows strong cytoplasmic positivity in melanocytes.
(1 Publication)
Species: Hu
Applications: WB, IHC, IHC-P
TRPC5 Antibody
(S67-15)
NBP2-12919
Western Blot: TRPC5 Antibody (S67-15) [NBP2-12919] - Rat Brain Membrane showing detection of ~110 kDa TrpC5 protein using Mouse Anti-TrpC5 Monoclonal Antibody, Clone S67-15. Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain Membrane cell lysate. Load: 20 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-TrpC5 Monoclonal Antibody ) at 1:1000 for 16 hours at 4 degrees C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:100 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~110 kDa. Other Band(s): 160kDa, 60kDa.
Immunohistochemistry: TRPC5 Antibody (S67-15) [NBP2-12919] - Tissue: Brain Slice. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-TrpC5 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT. Localization: Nuclear staining. Magnification: 10X.
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, IP, MiAr
Clusterin/APOJ
[HRP]
DCLU00
(2 Reviews)
(10 Publications)
Species: Hu
TRP7 Antibody
(S64A-36)
NBP1-48318
Western Blot: TRP7 Antibody (S64A-36) [NBP1-48318] - analysis of Rat brain membrane lysate showing detection of TrpC7 protein using Mouse Anti-TrpC7 Monoclonal Antibody, Clone S64A-36. Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpC7 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Immunocytochemistry/Immunofluorescence: TRP7 Antibody (S64A-36) [NBP1-48318] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-TrpC7 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Nuclear staining.
Immunohistochemistry-Paraffin: TRP7 Antibody (S64A-36) [NBP1-48318] - Mouse backskin sections. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP, MiAr
RanBP2 Antibody
NB120-2938
Western Blot: RanBP2 Antibody [NB120-2938] - Effect of hypoxia on the interaction between HIF1alpha and BICD1. Co-immunoprecipitation of HIF1alpha, Dynein IC, Importin alpha3, RanBP2 with IgG and BICD1 antibodies were shown in left panel. Total protein expressions in lysate were shown in right panel. n = 3. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41418-018-0241-1) licensed under a CC-BY licence.
Immunohistochemistry: RanBP2 Antibody [NB120-2938] - Figure 1 illustrates immunohistochemical staining of RanBP2 in bovine retina using NB120-2938. Figure 2 illustrates Western blot detection of RanBP2 from human retinal extracts using NB120-2938.
Western Blot: RanBP2 Antibody [NB120-2938] - Analysis of human retinal extracts.
(2 Publications)
Species: Hu, Mu, Bv
Applications: WB, IHC, IHC-P
Erythropoietin/EPO
[HRP]
MEP00B
(3 Reviews)
(77 Publications)
Species: Mu
IP3R1 Antibody
NBP1-21398
Western Blot: IP3R1 Antibody [NBP1-21398] - Whole cell lysate from HeLa cells. Affinity purified rabbit anti-IP3R1 antibody. IP3R1 was also immunoprecipitated by rabbit anti-IP3R1 antibody NBP1-21397.
Immunohistochemistry-Paraffin: IP3R1 Antibody [NBP1-21398] - Section of mouse cerebellum. Antibody: Affinity purified rabbit anti- IP3R1 used at a dilution of 1:100 (2ug/ml). Detection: Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight 594 Conjugated used ata dilution of 1:100. Counterstain: DAPI (blue).
Immunohistochemistry-Paraffin: IP3R1 Antibody [NBP1-21398] - Section of mouse cerebellum. Antibody: Affinity purified rabbit anti- IP3R1 used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
(1 Review)
(2 Publications)
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP, IF
Dystroglycan
Antibody
NBP2-14868
Western Blot: Dystroglycan Antibody [NBP2-14868] - Various tissue extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dystroglycan Antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: Dystroglycan Antibody [NBP2-14868] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Dystroglycan protein stained by Dystroglycan antibody 38) diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: Dystroglycan Antibody [NBP2-14868] - Mouse placenta. DAG1 stained by Dystroglycan antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
(2 Publications)
Species: Hu, Mu, Eq
Applications: WB, ICC/IF, IHC, IHC-P

Endorepellin/Perlecan/ ...
AF2364
Endorepellin/Perlecan was detected in immersion fixed paraffin-embedded sections of human skin using 5 µg/mL Goat Anti-Human Endorepellin/Perlecan Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2364) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
(5 Reviews)
(3 Publications)
Species: Hu
Applications: WB, IHC
IP3R3 Antibody
NBP1-21399
Immunohistochemistry-Paraffin: IP3R3 Antibody [NBP1-21399] - Section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-IP3R3 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: IP3R3 Antibody [NBP1-21399] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-IP3R3 antibody used for IP at 3 mcg/mg lysate. IP3R3 was also immunoprecipitated by rabbit anti-IP3R3 antibody NBP1-21400 which recognizes a downstream e
(1 Publication)
Species: Hu
Applications: WB (-), IHC, IHC-P
EPX Antibody
NBP2-13967
Western Blot: EPX Antibody [NBP2-13967] - Analysis in human cell line NB4.
Immunohistochemistry-Paraffin: EPX Antibody [NBP2-13967] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.
Immunohistochemistry-Paraffin: EPX Antibody [NBP2-13967] - Staining of human bone marrow shows moderate cytoplasmic positivity in hematopoietic cells.
(1 Publication)
Species: Hu
Applications: WB, IHC, IHC-P
TIMP-1 [HRP]
DTM100
(1 Review)
(81 Publications)
Species: Hu
TRPC2 Antibody
NBP2-80537
TRPC2 Antibody
Species: Mu
Applications: WB, IHC, IHC-P

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol TRPC2