TRPC6 Antibody

Western Blot: TRPC6 Antibody [NBP2-20737] - A549 whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRPC6 antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: TRPC6 Antibody [NBP2-20737] - Immunofluorescence analysis of methanol-fixed MCF-7, using antibody at 1:500 dilution.

Western Blot: TRPC6 Antibody [NBP2-20737] - Sample (30 ug of whole cell lysate) A: MCF-7 7. 5% SDS PAGE gel, diluted at 1:1000.

• Reactivity: Hu, Bv
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

TRPC6 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human TRPC6. The exact sequence is proprietary.
• Localization: Membrane; Multi-pass membrane protein
• Predicted Species: Bovine (91%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRPC6
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 106 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (88%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for TRPC6 Antibody

• FLJ11098
• FSGS2
• short transient receptor potential channel 6
• transient receptor potential cation channel, subfamily C, member 6
• Transient receptor protein 6
• TRP6
• TRP-6
• TRP6FLJ14863
• TRPC6

Background

The protein encoded by this gene forms a receptor-activated calcium channel in the cell membrane. The channel is activated by diacylglycerol and is thought to be under the control of a phosphatidylinositol second messenger system. Activation of this channel occurs independently of protein kinase C and is not triggered by low levels of intracellular calcium. Defects in this gene are a cause of focal segmental glomerulosclerosis 2 (FSGS2). [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: TRPC6
• Uniprot:
  • Q9Y210(Human)