TRPC6 Antibody - BSA Free

Western Blot: TRPC6 Antibody [NBP1-31008] - A549 whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRPC6 antibody diluted at 1:3000.

Immunohistochemistry-Paraffin: TRPC6 Antibody [NBP1-31008] - TRPC6 antibody [C3], C-term detects TRPC6 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse cerebellum.Green: TRPC6 stained by TRPC6 antibody [C3], C-term (NBP1-31008).Red: NF-H, a neuron marker, stained by NF-H antibody [GT114].Blue: Fluoroshield with DAPI.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: TRPC6 Antibody [NBP1-31008] - Sample(30 ug of whole cell lysate)A:HeLa S3 7.5% SDS PAGE, antibody diluted at 1:3000.

• Reactivity: Hu, Mu, Bv
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TRPC6 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TRPC6 Antibody - BSA Free (NBP1-31008) is a polyclonal antibody validated for use in IHC and WB. Anti-TRPC6 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human TRPC6. The exact sequence is proprietary.
• Localization: Membrane, Multi-pass membrane protein
• Predicted Species: Bovine (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRPC6
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 106 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 40% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for TRPC6 Antibody - BSA Free

• FLJ11098
• FSGS2
• short transient receptor potential channel 6
• transient receptor potential cation channel, subfamily C, member 6
• Transient receptor protein 6
• TRP6
• TRP-6
• TRP6FLJ14863
• TRPC6

Background

FUNCTION: Thought to form a receptor-activated non-selective calcium permanent cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C. Seems not to be activated by intracellular calcium store depletion. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for TRPC6 Antibody (NBP1-31008)(1)

Publication using NBP1-31008

• Chaudhuri P, Putta P, Rosenbaum MA, Graham LM p38 MAPK activation and STIM1-Orai3 association mediate TRPC6 externalization American journal of physiology. Cell physiology 2023-04-24 [PMID: 37093037]

Bioinformatics

• Gene Symbol: TRPC6