TROP-2 Antibody - BSA Free

Western Blot: TROP-2 Antibody [NBP1-92533] - Analysis in control (vector only transfected HEK293T lysate) and TACSTD2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunohistochemistry-Paraffin: TROP-2 Antibody [NBP1-92533] - Staining in human esophagus and colon tissues using anti-TACSTD2 antibody. Corresponding TACSTD2 RNA-seq data are presented for the same tissues.

Western Blot: TROP-2 Antibody [NBP1-92533] - Analysis in human cell line A-431.

Immunohistochemistry-Paraffin: TROP-2 Antibody [NBP1-92533] - Staining of human colon shows low expression as expected.

Immunohistochemistry-Paraffin: TROP-2 Antibody [NBP1-92533] - Staining of human esophagus shows high expression.

Immunohistochemistry-Frozen: TROP-2 Antibody [NBP1-92533] - Staining of LapC4 (Trop2 +) and PC3 (Trop2 -) tumor sections. This image was submitted via a customer review.

Analysis in human cell line A-431.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TROP-2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TROP-2 Antibody - BSA Free (NBP1-92533) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: SGMAVDCSTLTSKCLLLKARMSAPKNARTLVRPSEHALVDNDGL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TACSTD2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. IHC-F reported in a certified customer review.
• Control Peptide:

  TROP-2 Protein (NBP1-92533PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for TROP-2 Antibody - BSA Free

• Cell surface glycoprotein Trop-2
• EGP1
• EGP-1
• epithelial glycoprotein-1
• GA733-1
• GA733-1GP50
• gastrointestinal tumor-associated antigen GA7331
• gp50
• M1S1cell surface glycoprotein TROP2
• Membrane component chromosome 1 surface marker 1
• membrane component, chromosome 1, surface marker 1
• Pancreatic carcinoma marker protein GA733-1
• pancreatic carcinoma marker protein GA7331,40kD glycoprotein, identified by monoclonal GA733
• T16
• TACSTD2
• TROP2
• TROP-2
• TROP2GA7331
• tumor-associated calcium signal transducer 2

Background

TROP2 encodes a carcinoma-associated antigen defined by the monoclonal antibody GA733. This antigen is a member of a family including at least two type I membrane proteins. It transduces an intracellular calcium signal and acts as a cell surface receptor. Mutations of this gene result in gelatinous drop-like corneal dystrophy, an autosomal recessive disorder characterized by severe corneal amyloidosis leading to blindness. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for TROP-2 Antibody (NBP1-92533) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IHC-Fr Human 03/05/2018

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Human prostate cancer cell line
• Species: Human
• Lot: A105294

Bioinformatics

• Gene Symbol: TACSTD2