Claudin-1 Antibody (1C5-D9)

Images

 
Western Blot: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - CLDN1 monoclonal antibody (M01), clone 1C5-D9. Analysis of CLDN1 expression in A-431.
Immunohistochemistry-Paraffin: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - Analysis of monoclonal antibody to CLDN1 on formalin-fixed paraffin-embedded human colon. Antibody concentration 5 ug/ml.
Western Blot: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - CLDN1 monoclonal antibody (M01), clone 1C5-D9. Analysis of CLDN1 expression in PRC/PRF/5.
Western Blot: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - Analysis of CLDN1 expression in transfected 293T cell line by CLDN1 monoclonal antibody (M01), clone 1C5-D9.Lane 1: CLDN1 transfected lysate(23 KDa).Lane 2: ...read more
Immunohistochemistry-Paraffin: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - Analysis of monoclonal antibody to CLDN1 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.
Immunoprecipitation: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - Analysis of CLDN1 transfected lysate using anti-CLDN1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CLDN1 MaxPab rabbit ...read more
ELISA: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - Detection limit for recombinant GST tagged CLDN1 is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, Mu, PmSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP
Clone
1C5-D9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Claudin-1 Antibody (1C5-D9) Summary

Immunogen
CLDN1 (AAH12471.1, 1 a.a. ~ 211 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MANAGLQLLGFILAFLGWIGAIVSTALPQWRIYSYAGDNIVTAQAMYEGLWMSCVSQSTGQIQCKVFDSLLNLSSTLQATRALMVVGILLGVIAIFVATVGMKCMKCLEDDEVQKMRMAVIGGAIFLLAGLAILVATAWYGNRIVQEFYDPMTPVNARYEFGQALFTGWAAASLCLLGGALLCCSCPRKTTSYPTPRPYPKPAPSSGKDYV
Specificity
CLDN1 - claudin 1
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CLDN1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500
Application Notes
Antibody reactive against cell lysate, transfected lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA. Use in Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID:31769164).
Publications
Read Publications using
H00009076-M01 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Claudin-1 Antibody (1C5-D9)

  • claudin 1
  • Claudin1
  • Claudin-1
  • CLD1
  • CLDN1
  • ILVASCSenescence-associated epithelial membrane protein
  • SEMP1
  • SEMP1senescence-associated epithelial membrane protein 1

Background

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Claudin-1 Antibody (H00009076-M01)(25)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC/IF, WB, WB,ICC/IF.


Filter By Application
ICC/IF
(2)
WB
(3)
WB,ICC/IF
(1)
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Filter By Species
Human
(6)
All Species
Showing Publications 1 - 10 of 25. Show All 25 Publications.
Publications using H00009076-M01 Applications Species
Struver K, Friess W, Hedtrich S. et al. Development of a Perfusion Platform for Dynamic Cultivation of in vitro Skin Models. Skin Pharmacol Physiol Jun 27 2017 [PMID: 28651246]
Miki Y, Hamada K, Yoshino T et al. Type AB thymoma is not a mixed tumor of type A and type B thymomas, but a distinct type of thymoma. Virchows Arch. 2014 May 07 [PMID: 24802113]
Ratovitski EA. Phospho-DeltaNp63alpha regulates AQP3, ALOX12B, CASP14 and CLDN1 expression through transcription and microRNA modulation. FEBS Lett. 2013 Nov 1 [PMID: 24070899] (Human) Human
Kitazawa K, Kawasaki S, Shinomiya K et al. Establishment of a Human Corneal Epithelial Cell Line Lacking the Functional TACSTD2 Gene as an In Vitro Model for Gelatinous Drop-Like Dystrophy. Invest Ophthalmol Vis Sci. 2013 Aug 31 [PMID: 23868985]
Florentin J, Aouar B, Dental C et al. HCV glycoprotein E2 is a novel BDCA-2 ligand and acts as an inhibitor of IFN production by plasmacytoid dendritic cells. Blood. 2012 Oct 10 [PMID: 23053572]
Sainz B Jr, Barretto N, Yu X et al. Permissiveness of human hepatoma cell lines for HCV infection. Virol J. 2012 Jan 24 [PMID: 22273112]
Fletcher NF, Wilson GK, Murray J et al. Hepatitis C Virus Infects the Endothelial Cells of the Blood-Brain Barrier. Gastroenterology. 2011 Nov 30 [PMID: 22138189]
Kanki Y, Kohro T, Jiang S et al. Epigenetically coordinated GATA2 binding is necessary for endothelium-specific endomucin expression. EMBO J. 2011 Jun 10 [PMID: 21666600]
Jevtic M Fine-Tuning of Organotypic Skin Equivalents for Preclinical Research and Their Utilization to Study Epidermal-Dermal Crosstalk Thesis Jan 1 2021 (WB,ICC/IF) WB,ICC/IF
Yamamoto D, Kayamori K, Sakamoto K et al. Intracellular claudin-1 at the invasive front of tongue squamous cell carcinoma is associated with lymph node metastasis Cancer Sci. Feb 1 2020 [PMID: 31769164] (ICC/IF, Human) ICC/IF Human
Show All 25 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Claudin-1 Antibody (H00009076-M01). (Showing 1 - 1 of 1 FAQs).

  1. We are looking for two corresponding products, claudin 1 antibody and a peptide which can blocks the CLDN1.Could you plase kindly provide us solution for it? We found at your website one product, namely Claudin 1 Peptide ( NBP1-77036PEP),which can be used for blocking the activity of CLDN1. For the immunogen, 20 amino acids near the carboxy terminus of CLDN1 was mentioned. We found four Claudin 1 antibody ( NBP1-74546,NBP1-35652,NBP1-74517,H00009076-M1),and the immunogen was again the C-terminus of the Claudin 1.Cold you please send me more information about this C-terminus sequence?Is it the same in case of the NBP1-77036PEP and the four mentined antibodies?
    • The blocking peptide is a good purchase. The epitope for NBP1-35652 has not been mapped and the immunogen sequence is confidential, it is contained within the range between aa 140-211 of O95832 (Uniprot). Unfortunately NBP1-74517 has been discontinued. The amino acid sequence for H00009076-M01 is (AAH12471.1, 1 a.a. ~ 211 a.a). Unfortunately the lab that provides NBP1-74546 will not provide me with an aa range for the epitope as this is proprietary information that they are not willing to pass on.

Secondary Antibodies

 

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Nickel induces migratory and invasive phenotype in human epithelial cells by epigenetically activating ZEB1
By Jamshed Arslan Pharm.D. Nickel (Ni) is a naturally abundant metallic element. It is a major component of stainless steel, coins, and many other items of daily use. Disturbingly, Ni exposure is associated with can...  Read full blog post.

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Bioinformatics

Gene Symbol CLDN1
Entrez
OMIM
Uniprot