Western Blot: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - CLDN1 monoclonal antibody (M01), clone 1C5-D9. Analysis of CLDN1 expression in A-431.
Immunohistochemistry-Paraffin: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - Analysis of monoclonal antibody to CLDN1 on formalin-fixed paraffin-embedded human colon. Antibody concentration 5 ug/ml.
Western Blot: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - CLDN1 monoclonal antibody (M01), clone 1C5-D9. Analysis of CLDN1 expression in PRC/PRF/5.
Western Blot: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - Analysis of CLDN1 expression in transfected 293T cell line by CLDN1 monoclonal antibody (M01), clone 1C5-D9.Lane 1: CLDN1 transfected lysate(23 KDa).Lane 2: ...read more
Immunohistochemistry-Paraffin: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - Analysis of monoclonal antibody to CLDN1 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.
Immunoprecipitation: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - Analysis of CLDN1 transfected lysate using anti-CLDN1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CLDN1 MaxPab rabbit ...read more
ELISA: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - Detection limit for recombinant GST tagged CLDN1 is approximately 0.03ng/ml as a capture antibody.
CLDN1 (AAH12471.1, 1 a.a. ~ 211 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MANAGLQLLGFILAFLGWIGAIVSTALPQWRIYSYAGDNIVTAQAMYEGLWMSCVSQSTGQIQCKVFDSLLNLSSTLQATRALMVVGILLGVIAIFVATVGMKCMKCLEDDEVQKMRMAVIGGAIFLLAGLAILVATAWYGNRIVQEFYDPMTPVNARYEFGQALFTGWAAASLCLLGGALLCCSCPRKTTSYPTPRPYPKPAPSSGKDYV
CLDN1 - claudin 1
Test in a species/application not listed above to receive a full credit towards a future purchase.
Antibody reactive against cell lysate, transfected lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA. Use in Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID:31769164).
Read Publications using H00009076-M01 in the following applications:
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
In 1x PBS, pH 7.4
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Claudin-1 Antibody (1C5-D9)
ILVASCSenescence-associated epithelial membrane protein
SEMP1senescence-associated epithelial membrane protein 1
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Islam MJ, Amin MB, Uddin MKM et al. Mouse homologues of hepatitis C virus human entry factors inhibit the entry of HCV pseudoparticles (HCVpp) into human hepatoma cells. Bioresearch Communications Jan 1 2016
Islam MJ, Amin MB, Uddin MKM et al. Mouse homologues of hepatitis C virus human entry factors inhibit the entry of HCV pseudoparticles (HCVpp) into human hepatoma cells. Bioresearch Communications 2016 Jan 01 (WB, Human)
FAQs for Claudin-1 Antibody (H00009076-M01). (Showing 1 - 1 of 1 FAQs).
We are looking for two corresponding products, claudin 1 antibody and a peptide which can blocks the CLDN1.Could you plase kindly provide us solution for it? We found at your website one product, namely Claudin 1 Peptide ( NBP1-77036PEP),which can be used for blocking the activity of CLDN1. For the immunogen, 20 amino acids near the carboxy terminus of CLDN1 was mentioned. We found four Claudin 1 antibody ( NBP1-74546,NBP1-35652,NBP1-74517,H00009076-M1),and the immunogen was again the C-terminus of the Claudin 1.Cold you please send me more information about this C-terminus sequence?Is it the same in case of the NBP1-77036PEP and the four mentined antibodies?
The blocking peptide is a good purchase. The epitope for NBP1-35652 has not been mapped and the immunogen sequence is confidential, it is contained within the range between aa 140-211 of O95832 (Uniprot). Unfortunately NBP1-74517 has been discontinued. The amino acid sequence for H00009076-M01 is (AAH12471.1, 1 a.a. ~ 211 a.a). Unfortunately the lab that provides NBP1-74546 will not provide me with an aa range for the epitope as this is proprietary information that they are not willing to pass on.
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