Claudin-1 Antibody (1C5-D9)

Western Blot: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - CLDN1 monoclonal antibody (M01), clone 1C5-D9. Analysis of CLDN1 expression in A-431.

Immunohistochemistry-Paraffin: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - Analysis of monoclonal antibody to CLDN1 on formalin-fixed paraffin-embedded human colon. Antibody concentration 5 ug/ml.

Western Blot: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - CLDN1 monoclonal antibody (M01), clone 1C5-D9. Analysis of CLDN1 expression in PRC/PRF/5.

Western Blot: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - Analysis of CLDN1 expression in transfected 293T cell line by CLDN1 monoclonal antibody (M01), clone 1C5-D9.Lane 1: CLDN1 transfected lysate(23 KDa).Lane 2: Non-transfected lysate.

Immunohistochemistry-Paraffin: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - Analysis of monoclonal antibody to CLDN1 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.

Immunoprecipitation: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - Analysis of CLDN1 transfected lysate using anti-CLDN1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CLDN1 MaxPab rabbit polyclonal antibody.

ELISA: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - Detection limit for recombinant GST tagged CLDN1 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu, Mu, Pm
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP
• Clone: 1C5-D9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Claudin-1 Antibody (1C5-D9) Summary

• Immunogen: CLDN1 (AAH12471.1, 1 a.a. ~ 211 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MANAGLQLLGFILAFLGWIGAIVSTALPQWRIYSYAGDNIVTAQAMYEGLWMSCVSQSTGQIQCKVFDSLLNLSSTLQATRALMVVGILLGVIAIFVATVGMKCMKCLEDDEVQKMRMAVIGGAIFLLAGLAILVATAWYGNRIVQEFYDPMTPVNARYEFGQALFTGWAAASLCLLGGALLCCSCPRKTTSYPTPRPYPKPAPSSGKDYV
• Specificity: CLDN1 - claudin 1
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CLDN1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate, transfected lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA. Use in Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID:31769164).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Claudin-1 Antibody (1C5-D9)

• claudin 1
• Claudin1
• Claudin-1
• CLD1
• CLDN1
• ILVASCSenescence-associated epithelial membrane protein
• SEMP1
• SEMP1senescence-associated epithelial membrane protein 1

Background

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Claudin-1 Antibody (H00009076-M01)(25)

Publications using H00009076-M01

• Struver K, Friess W, Hedtrich S. et al. Development of a Perfusion Platform for Dynamic Cultivation of in vitro Skin Models. Skin Pharmacol Physiol 2017-06-27 [PMID: 28651246]
• Miki Y, Hamada K, Yoshino T et al. Type AB thymoma is not a mixed tumor of type A and type B thymomas, but a distinct type of thymoma. Virchows Arch. 2014-05-07 [PMID: 24802113]
• Ratovitski EA. Phospho-DeltaNp63alpha regulates AQP3, ALOX12B, CASP14 and CLDN1 expression through transcription and microRNA modulation. FEBS Lett. 2013-11-01 [PMID: 24070899] (Human)
• Kitazawa K, Kawasaki S, Shinomiya K et al. Establishment of a Human Corneal Epithelial Cell Line Lacking the Functional TACSTD2 Gene as an In Vitro Model for Gelatinous Drop-Like Dystrophy. Invest Ophthalmol Vis Sci. 2013-08-31 [PMID: 23868985]
• Florentin J, Aouar B, Dental C et al. HCV glycoprotein E2 is a novel BDCA-2 ligand and acts as an inhibitor of IFN production by plasmacytoid dendritic cells. Blood. 2012-10-10 [PMID: 23053572]
• Sainz B Jr, Barretto N, Yu X et al. Permissiveness of human hepatoma cell lines for HCV infection. Virol J. 2012-01-24 [PMID: 22273112]
• Fletcher NF, Wilson GK, Murray J et al. Hepatitis C Virus Infects the Endothelial Cells of the Blood-Brain Barrier. Gastroenterology. 2011-11-30 [PMID: 22138189]
• Kanki Y, Kohro T, Jiang S et al. Epigenetically coordinated GATA2 binding is necessary for endothelium-specific endomucin expression. EMBO J. 2011-06-10 [PMID: 21666600]
• Jevtic M Fine-Tuning of Organotypic Skin Equivalents for Preclinical Research and Their Utilization to Study Epidermal-Dermal Crosstalk Thesis 2021-01-01 (WB, ICC/IF)
• Yamamoto D, Kayamori K, Sakamoto K et al. Intracellular claudin-1 at the invasive front of tongue squamous cell carcinoma is associated with lymph node metastasis Cancer Sci. 2020-02-01 [PMID: 31769164] (ICC/IF, Human)
• Volz P, Schilrreff P, Brodwolf R et al. Pitfalls in using fluorescence tagging of nanomaterials: tecto-dendrimers in skin tissue as investigated by Cluster-FLIM. Ann. N. Y. Acad. Sci. 2017-10-01 [PMID: 28985028] (Human)
• Broussard EK, Kim R, Wiley JC et al. Identification of putative immunologic targets for colon cancer prevention based on conserved gene expression from pre-invasive to malignant lesions. Cancer Prev Res (Phila Pa) 2013-06-10 [PMID: 23682078] (WB, Human)
• Lowa A New biomedical approaches for studying (patho) physiological conditions of healthy and inflamed skin in vitro Thesis 2020-01-01 (ICC/IF, WB, Human)
• Giugliano S, Petroff MG, Warren BD et al. Hepatitis C Virus Sensing by Human Trophoblasts Induces Innate Immune Responses and Recruitment of Maternal NK Cells: Potential Implications for Limiting Vertical Transmission. J Immunol. 2015-09-04 [PMID: 26342030]
• Shaykhiev R, Otaki F, Bonsu P et al. Cigarette smoking reprograms apical junctional complex molecular architecture in the human airway epithelium in vivo. Cell Mol Life Sci. 2010-09-06 [PMID: 20820852]
• Islam MJ, Amin MB, Uddin MKM et al. Mouse homologues of hepatitis C virus human entry factors inhibit the entry of HCV pseudoparticles (HCVpp) into human hepatoma cells. Bioresearch Communications 2016-01-01
• Guevin C, Lamarre A, Labonte P. Novel HCV replication mouse model using human hepatocellular carcinoma xenografts. Antiviral Res 84(1):14-22. 2009-10-01 [PMID: 19622372]
• 1 Mee CJ, Grove J, Harris HJ et al. Effect of cell polarization on hepatitis C virus viral entry. J Virol;82(1):461-70. 2008-01-01 [PMID: 17959672]
• Islam MJ, Amin MB, Uddin MKM et al. Mouse homologues of hepatitis C virus human entry factors inhibit the entry of HCV pseudoparticles (HCVpp) into human hepatoma cells. Bioresearch Communications 2016-01-01 (WB, Human)
• Nakatsukasa M, Kawasaki S, Yamasaki K et al. Tumor-Associated Calcium Signal Transducer 2 Is Required for the Proper Subcellular Localization of Claudin 1 and 7. Implications in the Pathogenesis of Gelatinous Drop-Like Corneal Dystrophy. Am J Pathol. 2010-07-22 [PMID: 20651236]
• Sainz B Jr, TenCate V, Uprichard SL. Three-dimensional Huh7 cell culture system for the study of Hepatitis C virus infection. Virol J;6:103. 2009-07-15 [PMID: 19604376]
• Mee CJ, Harris HJ, Farquhar MJ et al. Polarization Restricts Hepatitis C Virus Entry into HepG2 Hepatoma Cells. J Virol;83(12):6211-6221. 2009-01-01 [PMID: 19357163]
• Stamataki Z, Shannon-Lowe C, Shaw J et al. Hepatitis C virus association with peripheral blood B lymphocytes potentiates viral infection of liver-derived hepatoma cells. Blood;113(3):585-593. 2009-01-01 [PMID: 18838615]
• Farquhar MJ, Harris HJ, Diskar M et al. Protein Kinase A-Dependent Step(s) in Hepatitis C Virus Entry Infectivity. J Virol;82(17):8797-8811. 2008-01-01 [PMID: 18579596]
• Harris HJ, Farquhar MJ, Mee CJ et al. CD81 Claudin 1 Coreceptor Association: Role in Hepatitis C Virus Entry. J Virol;82(10):5007-20. 2008-05-15 [PMID: 18337570]

Reviews for Claudin-1 Antibody (H00009076-M01) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Other, Pig.

reviewed by: L P ICC Pig 06/07/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: pig SC cells
• Species: Pig
• Lot: N/A

reviewed by: Anonymous - ICC Other 01/07/2016

Summary

• Application: Immunocytochemistry
• Sample Tested: SC cells
• Species: Other

FAQs for Claudin-1 Antibody (H00009076-M01). (Showing 1 - 1 of 1 FAQs).

1. We are looking for two corresponding products, claudin 1 antibody and a peptide which can blocks the CLDN1.Could you plase kindly provide us solution for it? We found at your website one product, namely Claudin 1 Peptide ( NBP1-77036PEP),which can be used for blocking the activity of CLDN1. For the immunogen, 20 amino acids near the carboxy terminus of CLDN1 was mentioned. We found four Claudin 1 antibody ( NBP1-74546,NBP1-35652,NBP1-74517,H00009076-M1),and the immunogen was again the C-terminus of the Claudin 1.Cold you please send me more information about this C-terminus sequence?Is it the same in case of the NBP1-77036PEP and the four mentined antibodies?
   • The blocking peptide is a good purchase. The epitope for NBP1-35652 has not been mapped and the immunogen sequence is confidential, it is contained within the range between aa 140-211 of O95832 (Uniprot). Unfortunately NBP1-74517 has been discontinued. The amino acid sequence for H00009076-M01 is (AAH12471.1, 1 a.a. ~ 211 a.a). Unfortunately the lab that provides NBP1-74546 will not provide me with an aa range for the epitope as this is proprietary information that they are not willing to pass on.

Bioinformatics

• Gene Symbol: CLDN1
• Entrez:
  • 9076(Human)
• OMIM:
  • 603718(Human)
• Uniprot:
  • AAH12471(Human)