EpCAM/TROP1 Antibody [Unconjugated]


Western blot shows lysates of HCT‑116 human colorectal carcinoma cell line, MCF‑7 human breast cancer cell line, and BG01V human embryonic stem cells. PVDF membrane was probed with 0.2 µg/mL of Goat ...read more
HT‑29 human colon adenocarcinoma cell line was stained with Goat Anti-Human EpCAM/TROP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF960, filled histogram) or isotype control antibody (Catalog # ...read more
EpCAM/TROP‑1 was detected in immersion fixed BG01V human embryonic stem cells using Goat Anti-Human EpCAM/TROP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF960) at 10 µg/mL for 3 hours at room ...read more
EpCAM/TROP‑1 was detected in immersion fixed paraffin-embedded sections of human colon using Goat Anti-Human EpCAM/TROP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF960) at 0.3 µg/mL for 1 hour ...read more
EpCAM/TROP‑1 was detected in immersion fixed paraffin-embedded sections of human colon using Goat Anti-Human EpCAM/TROP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF960) at 0.3 µg/mL for 1 ...read more
Simple Western lane view shows lysates of A549 human lung carcinoma cell line exosomes, HT‑29 human colon adenocarcinoma cell line exosomes, and COLO 205 human colorectal adenocarcinoma cell line whole cell ...read more
EpCAM/TROP‑1 was detected in immersion fixed HT‑29 human colon adenocarcinoma cell line (positive) and Jurkat human acute T cell leukemia cell line (negative) cells using Goat Anti-Human ...read more

Product Details

Reactivity HuSpecies Glossary
Applications WB, Simple Western, Flow, IHC, CyTOF-ready, ICC/IF

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EpCAM/TROP1 Antibody [Unconjugated] Summary

Mouse myeloma cell line NS0-derived recombinant human EpCAM/TROP-1, extracellular domain
Accession # CAA32870
Detects human EpCAM/TROP-1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human TROP-2 and approximately 26% cross-reactivity with recombinant mouse EpCAM/TROP-1 is observed.
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 0.3-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 0.2 ug/mL
Reviewed Applications
Read 1 Review rated 5
AF960 in the following applications:

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AF960 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EpCAM/TROP1 Antibody [Unconjugated]

  • 17-1A
  • 323/A3
  • ACSTD1
  • antigen identified by monoclonal AUA1
  • CD326 antigen
  • CD326
  • Cell surface glycoprotein Trop-1
  • chromosome 4, surface marker (35kD glycoprotein)
  • DIAR5
  • EGP
  • EGP-2
  • EGP314
  • EGP40
  • EpCAM
  • epithelial cell adhesion molecule
  • Epithelial cell surface antigen
  • Epithelial glycoprotein 314
  • Epithelial glycoprotein
  • ESA
  • GA733-2
  • GA733-2EGP34
  • gp40
  • hEGP314
  • HNPCC8
  • KS 1/4 antigen
  • KS1/4
  • KSAHEA125
  • M1S2
  • M4S1
  • M4S1Ly74
  • Major gastrointestinal tumor-associated protein GA733-2
  • MIC18MH99
  • MOC31
  • TACST-1
  • TROP1
  • TROP1CD326
  • Tumor-associated calcium signal transducer 1CO-17A


Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP-1, is a 40 kDa transmembrane glycoprotein composed of a 242 amino acid (aa) extracellular domain with two epidermal-growth-factor-like (EGF-like) repeats within the cysteine-rich N-terminal region, a 23 aa transmembrane domain, and a 26 aa cytoplasmic domain. Human and mouse EpCAM share 82% aa sequence identity. In human, EpCAM also shares 49% aa sequence homology with TROP-2/EGP-1. During embryonic development, EpCAM is detected in fetal lung, kidney, liver, pancreas, skin, and germ cells. In adults, human EpCAM is detected in basolateral cell membranes of all simple, pseudo-stratified, and transitional epithelia, but is not detected in normal squamous stratified epithelia, mesenchymal tissue, muscular tissue, neuro-endocrine tissue, or lymphoid tissue (1). EpCAM expression has been found to increase in actively proliferating epithelia tissues and during adult liver regeneration (1, 2). EpCAM expression is also found to increase in human malignant neoplasias, with most carcinoma expressing EpCAM including those of arising from squamous epithelia (1). EpCAM has been shown function as a homophilic Ca2+ independent adhesion molecule (3). Homophilic adhesion via EpCAM requires the interaction of both EGF-like repeats, with the first EGF-like repeat mediating reciprocal interaction between EpCAM molecules on opposing cells, while the second repeat is involved in lateral interaction of EpCAM. Lateral interaction of EpCAM lead to the formation of dimers and tetramers (4). During homophilic adhesion the cytoplasmic tail of EpCAM interacts with the actin cytoskeleton via a direct association alpha -actinin (5).

  1. Balzar, M. et al. (1999) J. Mol. Med. 77:699.
  2. Boer, C.J. et al. (1999) J. Pathol. 188:201.
  3. Litvinow, S.V. et al. (1994) J. Cell Biol. 125:437.
  4. Balzar, M. et al. (2001) Mol. Cell. Biol. 21:2570.
  5. Balzar, M. et al. (1998) Mol. Cell. Biol. 18:4388.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EpCAM/TROP1 Antibody (AF960)(15)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 9 applications: Adhesion Assay, CTC, Capture Assay, Cell Selection, CyTof, ELISA Capture, Flow Cytometry, ICC, IHC.

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Adhesion Assay
Capture Assay
Cell Selection
ELISA Capture
Flow Cytometry
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Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using AF960 Applications Species
Zhao, C;Wang, D;Li, Z;Zhang, Z;Xu, Y;Liu, J;Lei, Q;Han, D;Huo, Y;Liu, S;Li, L;Zhang, Y; IL8 derived from macrophages inhibits CD8+ T-cell function by downregulating TIM3 expression through IL8-CXCR2 axis in patients with advanced colorectal cancer International immunopharmacology 2023-06-16 [PMID: 37331296] (IHC, ICC, Human) IHC, ICC Human
Matkovic Leko, I;Schneider, RT;Thimraj, TA;Schrode, N;Beitler, D;Liu, HY;Beaumont, K;Chen, YW;Snoeck, HW; A distal lung organoid model to study interstitial lung disease, viral infection and human lung development Nature protocols 2023-05-10 [PMID: 37165073] (IHC, Human) IHC Human
IM Leko, N Schrode, J Torres, M Pezet, TA Thimraj, KG Beaumont, HW Snoeck Generation and expansion of transitional lung organoids from human pluripotent stem cells bioRxiv : the preprint server for biology, 2023-02-01;0(0):. 2023-02-01 [PMID: 36778291] (ICC, Human) ICC Human
JJ Wang, N Sun, YT Lee, M Kim, T Vagner, K Rohena-Riv, Z Wang, Z Chen, RY Zhang, J Lee, C Zhang, H Tang, J Widjaja, TX Zhang, D Qi, PC Teng, YJ Jan, KC Hou, C Hamann, HM Sandler, TJ Daskivich, DJ Luthringer, NA Bhowmick, R Pei, S You, DD Vizio, HR Tseng, JF Chen, Y Zhu, EM Posadas Prostate cancer extracellular vesicle digital scoring assay - a rapid noninvasive approach for quantification of disease-relevant mRNAs Nano Today, 2023-01-03;48(0):. 2023-01-03 [PMID: 36711067] (Capture Assay, Human) Capture Assay Human
H Yang, Q Deng, T Ni, Y Liu, L Lu, H Dai, H Wang, W Yang Targeted Inhibition of LPL/FABP4/CPT1 fatty acid metabolic axis can effectively prevent the progression of nonalcoholic steatohepatitis to liver cancer International journal of biological sciences, 2021-10-11;17(15):4207-4222. 2021-10-11 [PMID: 34803493] (IHC, Mouse) IHC Mouse
J Zhang, LTH Nguyen, R Hickey, N Walters, X Wang, KJ Kwak, LJ Lee, AF Palmer, E Reátegui Immunomagnetic sequential ultrafiltration (iSUF) platform for enrichment and purification of extracellular vesicles from biofluids Scientific Reports, 2021-04-13;11(1):8034. 2021-04-13 [PMID: 33850163] (Flow Cytometry, ICC, Human) Flow Cytometry, ICC Human
KM Chan, J Gleadle, J Li, TD Michl, K Vasilev, M MacGregor Improving hexaminolevulinate enabled cancer cell detection in liquid biopsy immunosensors Scientific Reports, 2021-03-31;11(1):7283. 2021-03-31 [PMID: 33790357] (ELISA Capture, Human) ELISA Capture Human
LL Lin, ER Kost, CL Lin, P Valente, CM Wang, MG Kolonin, AC Daquinag, X Tan, N Lucio, CN Hung, CP Wang, NB Kirma, TH Huang PAI-1-Dependent Inactivation of SMAD4-Modulated Junction and Adhesion Complex in Obese Endometrial Cancer Cell Rep, 2020-10-13;33(2):108253. 2020-10-13 [PMID: 33053339] (CyTof, Human) CyTof Human
N Sun, YT Lee, RY Zhang, R Kao, PC Teng, Y Yang, P Yang, JJ Wang, M Smalley, PJ Chen, M Kim, SJ Chou, L Bao, J Wang, X Zhang, D Qi, J Palomique, N Nissen, SB Han, S Sadeghi, RS Finn, S Saab, RW Busuttil, D Markovic, D Elashoff, HH Yu, H Li, AP Heaney, E Posadas, S You, JD Yang, R Pei, VG Agopian, HR Tseng, Y Zhu Purification of HCC-specific extracellular vesicles on nanosubstrates for early HCC detection by digital scoring Nat Commun, 2020-09-07;11(1):4489. 2020-09-07 [PMID: 32895384] (ELISA Capture, Human) ELISA Capture Human
S Yoshida, H Miwa, T Kawachi, S Kume, K Takahashi Generation of intestinal organoids derived from human pluripotent stem cells for drug testing Sci Rep, 2020-04-06;10(1):5989. 2020-04-06 [PMID: 32249832] (ICC, Human) ICC Human
Show All 15 Publications.

Review for EpCAM/TROP1 Antibody (AF960) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF960:
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Images Ratings Applications Species Date Details
Immunocytochemistry EpCAM/TROP1 AF960
reviewed by:
Pauline Jeannot
ICC Human 11/16/2023


Sample TestedMDA MB 231 cells

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FAQs for EpCAM/TROP1 Antibody (AF960). (Showing 1 - 1 of 1 FAQs).

  1. I'm looking for EpCAM antibody for rat. Do you have any antibody which can use FACS?
    • We unfortunately do not have any EpCAM antibodies validated in FACS for detection of the rat protein. I am sorry for any inconvenience. I do have three products to EpCAM that can detect the rat protein. These products have been validated to detect the native rat protein, so they may work for FACS however they have not yet been tested in that particular application. If you would like to try them in your experiment you would qualify for our Innovator's Reward Program. This program was designed to help scientists with the cost of their antibodies in return for valuable information. If you decide to test an antibody in a previously untested species or application we will issue you a credit for the purchase price of the antibody in exchange for a review of your experiment using our product.

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Blogs on EpCAM/TROP1.

Stemness is responsible for onset and metastasis of colorectal cancer
By Jamshed Arslan, Pharm. D., PhD. Colorectal cancer stem cells are a rare subpopulation of colorectal cancer cells that can self-renew and initiate and sustain tumor growth when transplanted into an animal host.1,2 C...  Read full blog post.

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Recent Reviews


Pauline Jeannot
Application: ICC
Species: Human


Gene Symbol EPCAM