Cytokeratin 3 Antibody (KRT3/2130)

Images

 
Immunohistochemistry-Paraffin: Cytokeratin 3 Antibody (KRT3/2130) [NBP2-79718] - Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin 3 Antibody (KRT3/2130).
Immunohistochemistry-Paraffin: Cytokeratin 3 Antibody (KRT3/2130) [NBP2-79718] - Formalin-fixed, paraffin-embedded human Ovary stained with Cytokeratin 3 Antibody (KRT3/2130).
Protein Array: Cytokeratin 3 Antibody (KRT3/2130) [NBP2-79718] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Cytokeratin 3 Antibody (KRT3/2130). Z- and S- Score: The Z-score ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, PA
Clone
KRT3/2130
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Cytokeratin 3 Antibody (KRT3/2130) Summary

Immunogen
Recombinant full-length human Cytokeratin 3 protein (Uniprot: P12035)
Localization
Cytoplasmic
Marker
Corneal Epithelial Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
KRT3
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
  • Western Blot 1:2000
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79852)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for Cytokeratin 3 Antibody (KRT3/2130)

  • CK3
  • CK-3
  • cytokeratin-3
  • FLJ95909
  • K3cytokeratin 3
  • keratin 3
  • keratin, type II cytoskeletal 3,65 kDa cytokeratin
  • Keratin-3
  • Type-II keratin Kb3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol KRT3
Uniprot