Occludin Antibody

Images

 
Orthogonal Strategies: Western Blot: Occludin Antibody [NBP1-87402] - Analysis in human cell line CACO-2 and human cell line HeLa.
Genetic Strategies: Western Blot: Occludin Antibody [NBP1-87402] - Lane 1: untreated vascular endothelial cells Lane 2: vascular endothelial cells + control siRNA. Primary antibody was diluted 1:1000. WB image ...read more
Immunocytochemistry/ Immunofluorescence: Occludin Antibody [NBP1-87402] - Staining of IEC-6 rat intestinal epithelial cells using Occludin antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: Occludin Antibody [NBP1-87402] - Staining of human prostate shows negative membranous positivity in glandular cells as expected.
Immunocytochemistry/ Immunofluorescence: Occludin Antibody [NBP1-87402] - Methanol fixed, mouse choroid plexus cells were stained with Occludin antibody. ICC/IF image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: Occludin Antibody [NBP1-87402] - Staining of human cell line A-431 shows localization to plasma membrane & cell junctions. Antibody staining shown in green.
Immunohistochemistry-Paraffin: Occludin Antibody [NBP1-87402] - Staining of human breast cancer shows moderate membranous positivity in tumor cells.
Immunohistochemistry-Paraffin: Occludin Antibody [NBP1-87402] - Staining of human prostate cancer shows moderate membranous positivity in tumor cells.
Immunohistochemistry-Paraffin: Occludin Antibody [NBP1-87402] - Staining of human stomach shows moderate membranous positivity in glandular cells.
ELISA: Occludin Antibody [NBP1-87402] - Occludin levels in untreated and LPS treated rat intestinal tissues. ELISA image submitted by a verified customer review.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Occludin Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: DKEHIYDEQPPNVEEWVKNVSAGTQDVPSPPSDYVERVDSPMAYSSNGKVNDKRFYPESSYKSTPVPEVVQELPLTSPVDDFRQPRYSSGGNFETPSKRAPAKGRAGRSKRTEQDHYETDYTTGGESCDELEED
Marker
Tight Junctions Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
OCLN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/Immunofluorescence 0.25 - 2 ug/mL
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Knockdown Validated
  • Western Blot 0.04 - 0.4 ug/mL
Application Notes
ICC/IF reported in scientific literature (PMID: 28848383), and a verified customer review. For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. Occludin antibody validated for WB and ELISA from a verified customer reviews.
Control Peptide
Occludin Protein (NBP1-87402PEP)
Reviewed Applications
Read 4 Reviews rated 4
using
NBP1-87402 in the following applications:

Publications
Read Publications using
NBP1-87402 in the following applications:

  • 4 publications
  • IHC
    5 publications
  • 2 publications
  • WB
    2 publications

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 31715313). Mouse reactivity reported in (PMID: 30685438), and a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2), 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Occludin Antibody

  • BLCPMG
  • Occludin
  • OCLN

Background

The Occludin gene encodes an integral membrane protein which is located at tight junctions. This protein may be involved in the formation and maintenance of the tight junction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Occludin Antibody (NBP1-87402)(15)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-P, WB.


Filter By Application
ICC/IF
(4)
IHC
(5)
IHC-P
(2)
WB
(2)
All Applications
Filter By Species
Human
(4)
Mouse
(6)
Rat
(1)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using NBP1-87402 Applications Species
Li Y, Wei JY, Liu H et al. An oxygen-adaptive interaction between SNHG12 and occludin maintains blood-brain barrier integrity Cell reports Apr 12 2022 [PMID: 35417709] (ICC/IF, Human) ICC/IF Human
Pretorius L, Van Staden ADP, Van der Merwe JJ et al. Alterations to microbial secretome by estrogen may contribute to sex bias in irritable bowel syndrome Inflammopharmacology Jan 13 2022 [PMID: 35022916]
Hua Q, Han Y, Zhao H et al. Punicalagin alleviates renal injury via the gut-kidney axis in high-fat diet-induced diabetic mice Food & function Jan 6 2022 [PMID: 34989745] (IHC-P) IHC-P
Delaney C, Farrell M, Doherty CP et al. Attenuated CSF-1R signalling drives cerebrovascular pathology EMBO molecular medicine Dec 22 2020 [PMID: 33350588] (WB, Human, Mouse) WB Human, Mouse
Heldt NA, Seliga A, Winfield M et al. Electronic cigarette exposure disrupts blood-brain barrier integrity and promotes neuroinflammation Brain Behav. Immun. Mar 31 2020 [PMID: 32243899] (IHC, Mouse) IHC Mouse
Shinde T, Perera AP, Vemuri R et al. Synbiotic supplementation with prebiotic green banana resistant starch and probiotic Bacillus coagulans spores ameliorates gut inflammation in mouse model of inflammatory bowel diseases Eur J Nutr Feb 17 2020 [PMID: 32067099] (IHC, Mouse) IHC Mouse
Shastri S, Shinde T, Sohal SS et al. Idebenone Protects against Acute Murine Colitis via Antioxidant and Anti-Inflammatory Mechanisms Int J Mol Sci Jan 12 2020 [PMID: 31940911] (IHC, Mouse) IHC Mouse
Almassy J, Diszhazi G, Skaliczki M et al. Expression of BK channels and Na+-K+ pumps in the apical membrane of lacrimal acinar cells suggests a new molecular mechanism for primary tear-secretion. Ocul Surf Jan 24 2019 [PMID: 30685438] (ICC/IF, Mouse) ICC/IF Mouse
Messal N, Fernandez N, Dayot S et al. Ectopic expression of OX1R in ulcerative colitis mediates anti-inflammatory effect of orexin-A Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease Nov 1 2018 [PMID: 30251681] (WB, Mouse) WB Mouse
Rom S, Zuluaga-Ramirez V, Gajghate S et al. Hyperglycemia-Driven Neuroinflammation Compromises BBB Leading to Memory Loss in Both Diabetes Mellitus (DM) Type 1 and Type 2 Mouse Models. Mol. Neurobiol. Jul 5 2018 [PMID: 29974394] (IHC) IHC
Show All 15 Publications.

Reviews for Occludin Antibody (NBP1-87402) (4) 44

Average Rating: 4
(Based on 4 reviews)
We have 4 reviews tested in 2 species: Mouse, Rat.

Reviews using NBP1-87402:
Filter by Applications
ICC
(1)
ELISA
(1)
WB
(1)
IF
(1)
All Applications
Filter by Species
Mouse
(2)
Rat
(2)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry Occludin NBP1-87402
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3
reviewed by:
Verified Customer
ICC Rat 04/06/2022
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Summary

ApplicationImmunocytochemistry
Sample TestedIEC-6 rat intestinal epithelial cells
SpeciesRat
Lot10666
ELISA Occludin NBP1-87402
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4
reviewed by:
Russ Tas
ELISA Rat 06/01/2021
View

Summary

ApplicationELISA
Sample Testedrat intestine
SpeciesRat
Lot000010666
Western Blot Occludin NBP1-87402
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5
reviewed by:
Verified Customer
WB Mouse 06/15/2017
View

Summary

ApplicationWestern Blot
Sample TestedPrimary lung endothelial cells
SpeciesMouse
LotB106982

Comments

CommentsMembrane was blocked in 5% milk in TBST. Primary antibody dilution was 1:1000 and the dilution for the secondary ab was 1:5000.
Immunofluorescence Occludin NBP1-87402
Enlarge
4
reviewed by:
Nadine Bakkar
IF Mouse 04/06/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedMouse choroid plexus cells
SpeciesMouse

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Occludin Antibody (NBP1-87402) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Occludin Antibody (NBP1-87402)

Discover related pathways, diseases and genes to Occludin Antibody (NBP1-87402). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Recent Reviews

4
5
1
4
2
3
1
2
0
1
0

Verified Customer
04/06/2022
Application: ICC
Species: Rat

Russ Tas
06/01/2021
Application: ELISA
Species: Rat

Verified Customer
06/15/2017
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol OCLN