TrkB [p Tyr707, p Tyr706] Antibody - BSA Free

Immunohistochemistry-Paraffin: TrkB [p Tyr706] Antibody [NBP2-54764] - Human brain tissue using Trk B [P-Tyr706/Tyr707) antibody (left) or the same antibody preincubated with blocking peptide (right).

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

TrkB [p Tyr707, p Tyr706] Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TrkB [p Tyr707, p Tyr706] Antibody - BSA Free (NBP2-54764) is a polyclonal antibody validated for use in IHC and WB. Anti-TrkB Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Trk B (phospho Tyr706/Tyr707) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 706/tyrosine 707 (T-D-Y (p)-Y (p)-R-V)derived from Human Trk B.
• Modification: p Tyr706, p Tyr707
• Specificity: The antibody detects endogenous levels of TrkB only when phosphorylated at tyrosine 706 and tyrosine 707.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NTRK2
• Purity: Epitope affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10 - 1:200
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Western Blot
• Application Notes: This TrkB [p Tyr706] Antibody is validated for WB from a verified customer review.
• Theoretical MW: 91 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 °C.
• Buffer: PBS (pH 7.4) (without Mg2+ and Ca2+), 150mM NaCl, 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Epitope affinity purified

Alternate Names for TrkB [p Tyr707, p Tyr706] Antibody - BSA Free

• BDNF/NT-3 growth factors receptor
• EC 2.7.10
• EC 2.7.10.1
• GP145-TrkB
• Neurotrophic tyrosine kinase receptor type 2
• neurotrophic tyrosine kinase, receptor, type 2
• NTRK2
• TrkB tyrosine kinase
• TrkB
• trk-B
• TRKBBDNF-tropomyosine receptor kinase B
• tyrosine kinase receptor B

Background

TrkB is a tyrosine-protein kinase receptor involved in the development and/or maintenance of the nervous system. TrkB is a receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5, but not nerve growth factor (NGF). The major substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TrkB Antibody (NBP2-54764)(4)

Publications using NBP2-54764

• Mina Sadighi, Lingling Mai, Yifan Xu, Morgane Boillot, Giorgia Targa, Francesca Mottarlini, Paolo Brambilla, Peter Gass, Lucia Caffino, Fabio Fumagalli, Judith R Homberg Chronic exposure to imipramine induces a switch from depression-like to mania-like behavior in female serotonin transporter knockout rats: Role of BDNF signaling in the infralimbic cortex. Journal of affective disorders 2024-02-28 [PMID: 38280571]
• Caffino L, Mottarlini F, Piva A et al. Temporal dynamics of BDNF signaling recruitment in the rat prefrontal cortex and hippocampus following a single infusion of a translational dose of ketamine Neuropharmacology 2023-10-17 [PMID: 37858883] (WB, Rat)
  
  Details:
  1:200 WB diltuion
• Mottarlini F, Rizzi B, Targa G et al. Long-lasting BDNF signaling alterations in the amygdala of adolescent female rats exposed to the activity-based anorexia model Frontiers in behavioral neuroscience 2022-12-08 [PMID: 36570702] (WB, Rat)
  
  Details:
  Dilution used 1:200
• Mottarlini F, Fumagalli M, Castillo-DIaz F et al. Single and Repeated Exposure to Cannabidiol Differently Modulate BDNF Expression and Signaling in the Cortico-Striatal Brain Network Biomedicines 2022-08-01 [PMID: 36009400] (WB, Rat)
  
  Details:
  WB 1:500

Review for TrkB Antibody (NBP2-54764) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: francesca mottarlini WB Rat 02/19/2019

Summary

• Application: Western Blot
• Sample Tested: Rat brain lysate (ventral hippocampus),Rat brain synaptosomal (P2) fraction,Rat brain tissue
• Species: Rat
• Lot: 22012-1804

Comments

• Comments: I’m writing to inform you about the Western Blot trial that I have done regarding the pTyr706 NBP2-54764; antibody that I bought Order n° 1334 15/11/2018 and that was untested for WB analysis.
  First of all, in the datasheet that you provide, bands of trkB are expected at 95kDa. However, we know from the literature that trkB receptor is present in two forms: the full length that weights 145 kDa and the truncated one that weights 95 kDa. As you can see from the blots attached, I effectively obtained with your antibody (against the phosphorilated form) and with an antibody against the total form of trkB (cell signaling #4603) two different bands at these specific molecular weights.
  
  The test was conducted on rat brain prefrontal cortex tissue extracts (Figure 1-4), and on rat brain hippocampus (Figure 5-6) obtained following the below-mentioned protocol.
  Prefrontal cortex (Pfc) tissues were processed following a classic protein extraction method done at room temperature, which allow us to obtain aliquots of whole homogenate (HOMO), nuclei (P1), cytosol (S2) and a crude membrane fractions (P2).
  
  1. Pfc tissues were homogenized in a glass-glass potter in a homogenization buffer (0,32 M sucrose, 0,1 mM PMSF, 1 mM Hepes, 0,1 mM EGTA pH 7,4, 1X Complete (protease inhibitor cocktail),
  1X phosphatase inhibitor cocktail).
  From that step we kept an aliquot of homogenate, we sonicated it and maintained at -20°C for following WB analysis.
  
  2. The left homogenate was moved on through the extraction and centrifuged 10 minutes at 4°C at 1000g.
  
  3. The obtained surnatant S1 was again centrifuged 15 minutes at 4°C at 9000g to obtain the P2 and the S2, while the pellet was the P1 fraction that was resuspended in a buffer solution (20 mM
  Hepes, 0,1 mM DTT (ditiotreitol), 0,1 mM, EGTA pH 7,4, 1X Complete, 1X phosphatase inhibitor cocktail). The obtained P2 was as for the P1 resuspended in the same previous buffer.
  Protein extracts were separated by polyacrylamide SDS/PAGE and transferred to nitrocellulose membranes. Blots were immunostained overnight at 4°C with the specific primary antibodies (see figures for specifics). After washing, the membranes were incubated for 1 h at room temperature with the appropriate secondary antibody (anti-mouse, anti-rabbit). After secondary antibody incubations, membranes were washed and finally incubated with ECL detection reagent.
  Western blot-1:
  - Figure1: blot of pTrkB Tyr706 measured in rat brain extracts of Prefrontal cortex in the whole homogenate (HOMO) and crude synaptosomal fraction (P2). We revealed one band at 145 kDa, molecular weight of Full length TrkB.
  - Figure2: blot of pTrkB Tyr706 measured in rat brain extracts of Prefrontal cortex in the whole homogenate (HOMO) and crude synaptosomal fraction (P2). We revealed one band at 95 kDa, molecular weight of truncated TrkB.
  - Figure3: blot of total TrkB measured in rat brain extracts of Prefrontal cortex in the whole homogenate (HOMO) and crude synaptosomal fraction (P2). Primary TrkB antibody (#4603) Cell signalling
  - Figure4: blot of beta-Actin (Molecular weight 43 kDa) - Prefrontal cortex
  
  Western blot-2:
  - Figure5: blot of pTrkB Tyr706 measured in rat brain extracts of hippocampus in the whole homogenate (HOMO). We revealed two bands: one at 145 kDa molecular weight of the TrkB full length and one at 95 kDa (truncated TrkB).
  - Figure6: blot of total TrkB measured in rat brain extracts of hippocampus in the whole homogenate (HOMO). We revealed two bands: one at 145 kDa molecular weight of the TrkB full length and one at 95 kDa (truncated TrkB).
  
  - Figure7: blot of beta-Actin (Molecular weight 43 kDa) - Hippocampus

Bioinformatics

• Gene Symbol: NTRK2