TrkB Antibody - Azide Free

Western Blot: TrkB Antibody [NBP2-29660] - rat synaptic membrane preparation showing specific immunolabeling of the ~ 93k TrkB protein.

• Reactivity: Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

TrkB Antibody - Azide Free Summary

• Description: Novus Biologicals Rabbit TrkB Antibody - Azide Free (NBP2-29660) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH. Accession # Q63604
• Specificity: Specific for the ~93 kDa TrkB protein in Western blots.
• Preparation Method: Prepared from rabbit serum by affinity purification on a column made with the C-terminal peptide used as antigen.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NTRK2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 93 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Affinity purified

Alternate Names for TrkB Antibody - Azide Free

• BDNF/NT-3 growth factors receptor
• EC 2.7.10
• EC 2.7.10.1
• GP145-TrkB
• Neurotrophic tyrosine kinase receptor type 2
• neurotrophic tyrosine kinase, receptor, type 2
• NTRK2
• TrkB tyrosine kinase
• TrkB
• trk-B
• TRKBBDNF-tropomyosine receptor kinase B
• tyrosine kinase receptor B

Background

Tropomyosin-receptor-kinase (TRK) receptors are members of the receptor tyrosine kinase family and include TrkA, TrkB and TrkC. Each of these receptor types has different binding affinities to the neurotrophins nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF) and neurotrophin 3 (NT-3). TrkB has the highest affinity to binding BDNF which plays critical roles in the function and survival of neurons in the CNS (Soppet et al., 1991; Klein et al., 1991). Additionally, alterations in expression of TrkB have been associated with Alzheimer's disease (Ferrer et al., 1999; Chen et al., 2008).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NTRK2
• Entrez:
  • 25054(Rat)
• Uniprot:
  • Q63604(Rat)