TRIM3/BERP Antibody

Images

 
Western Blot: TRIM3/BERP Antibody [NB100-2494] - Staining of Human Cerebellum (A) and Rat (B) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: TRIM3/BERP Antibody [NB100-2494] - HEK293 overexpressing TRIM3 and probed with (mock transfection in first lane).
Western Blot: TRIM3/BERP Antibody [NB100-2494] - Western blot analysis of TRIM3/BERP in Mouse Brain lysate (RIPA buffer, 30ug total protein per lane) using NB100-2494 at 0.05ug/ml. Primary incubated for 1 hour. Detected ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, CaSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

TRIM3/BERP Antibody Summary

Immunogen
Peptide with sequence AKREDSPGPEVQP-C corresponding to N-Terminus according to NP_150594.2.
Specificity
Both variants (NP_006449.2; NP_150594.2;) represent the same protein. This antibody is expected to recognise all three human isoforms of this protein.
Predicted Species
Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TRIM3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 1 ug/ml
  • Peptide ELISA Detection limit 1:64000
Application Notes
Please note the HEK cell experiements were performed using a previous batch (different goat). WB: Approx. 85 kDa band observed in mouse brain and human brain (cerebellum) lysate (predicted MW of approx. 80.8 kDa band according to NP_006449.2).
Control
TRIM3/BERP Overexpression Lysate
Mouse Brain Whole Tissue Lysate (Adult Whole Normal)
Publications
Read Publication using NB100-2494.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TRIM3/BERP Antibody

  • BERPRING finger protein 97
  • Brain-expressed RING finger protein
  • HAC1
  • RING finger protein 22
  • RNF22brain expressed ring finger
  • RNF97FLJ16135
  • tripartite motif containing 3
  • tripartite motif protein TRIM3
  • tripartite motif-containing 3
  • tripartite motif-containing protein 3

Background

Gene ID(s): 10612 (human)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TRIM3/BERP Antibody (NB100-2494)(1)

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Video Protocols

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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol TRIM3
Entrez
Uniprot