TRIM3/BERP Antibody

Western Blot: TRIM3/BERP Antibody [NB100-2494] - Staining of Human Cerebellum (A) and Rat (B) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western Blot: TRIM3/BERP Antibody [NB100-2494] - HEK293 overexpressing TRIM3 and probed with (mock transfection in first lane).

Western Blot: TRIM3/BERP Antibody [NB100-2494] - Western blot analysis of TRIM3/BERP in Mouse Brain lysate (RIPA buffer, 30ug total protein per lane) using NB100-2494 at 0.05ug/ml. Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

TRIM3/BERP Antibody Summary

• Immunogen: Peptide with sequence AKREDSPGPEVQP-C corresponding to N-Terminus according to NP_150594.2.
• Specificity: Both variants (NP_006449.2; NP_150594.2;) represent the same protein. This antibody is expected to recognise all three human isoforms of this protein.
• Predicted Species: Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TRIM3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.1 - 1 ug/ml
• Application Notes: Please note the HEK cell experiements were performed using a previous batch (different goat). WB: Approx. 85 kDa band observed in mouse brain and human brain (cerebellum) lysate (predicted MW of approx. 80.8 kDa band according to NP_006449.2).
• Control:

  TRIM3/BERP Overexpression Lysate
  Mouse Brain Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TRIM3/BERP Antibody

• BERPRING finger protein 97
• Brain-expressed RING finger protein
• HAC1
• RING finger protein 22
• RNF22brain expressed ring finger
• RNF97FLJ16135
• tripartite motif containing 3
• tripartite motif protein TRIM3
• tripartite motif-containing 3
• tripartite motif-containing protein 3

Background

Gene ID(s): 10612 (human)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRIM3/BERP Antibody (NB100-2494)(1)

Publication using NB100-2494

• El-Husseini AE, Fretier P, Vincent SR. Cloning and characterization of a gene (RNF22) encoding a novel brain expressed ring finger protein (BERP) that maps to human chromosome 11p15.5. Genomics 2001-02-01 [PMID: 11170753]

Bioinformatics

• Gene Symbol: TRIM3
• Entrez:
  • 10612(Human)
  • 55992(Mouse)
  • 83616(Rat)
• Uniprot:
  • NP_006449.2(Human)