FE65 Antibody - BSA Free

Western Blot: FE65 Antibody [NB110-58360] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY APBB1 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-APBB1.

Western Blot: FE65 Antibody [NB110-58360] - Protein on HEK 293 cell lysate.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.3 mg/ml

FE65 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit FE65 Antibody - BSA Free (NB110-58360) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant rat FE65, expressed in bacteria. [Swiss-Prot# P46933]
• Localization: Cytoplasmic
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: APBB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-10 ug
  • Western Blot 1:1000
• Application Notes: This FE65 antibody is useful in Western blot and Immunoprecipitation. In Western blot a band is seen at ~100 kDa versus the predicted molecular weight of 77 kDa, which corresponds to earlier findings in literature (PMID: 9685356). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 100 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  FE65 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 15% glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.3 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FE65 Antibody - BSA Free

• adaptor protein FE65a2
• amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65)
• amyloid beta A4 precursor protein-binding, family B, member 1
• Fe65
• FE65amyloid beta A4 precursor protein-binding family B member 1
• MGC:9072
• Protein Fe65
• RIR
• stat-like protein

Background

Fe65 (Protein Fe65 or APBB1) is a neuronal adaptor protein that mediates the assembly of multimolecular complexes through a variety of protein-protein interaction domains: the WW domain, which binds to proline-rich sequences, and two C-terminal phosphotyrosine-binding domains. FE65 is primarily found in CNS and is highly expressed in neurons of hippocampus, cerebellum, thalamus, and brain stem nuclei. Fe65 is localized within cytoplasm or nucleus, although its moderate amounts are tethered to membranes via binding with Alzheimer's disease amyloid precursor protein (APP). FE65 forms stable, transcriptionally active complex with AICD (APP intracellular domain) that follows nuclear translocation of AICD and apoptosis through Tip60. FE65 is functionally linked to cellular motility, morphology and actin dynamics through binding of its WW domain to actin-binding protein Mena. It plays a key role in response to DNA damage by translocating to nucleus and inducing apoptosis through recognizing/binding histone H2AX phosphorylated on 'Tyr-142' (H2AXY142ph) at DSBs, recruiting other pro-apoptosis factors such as MAPK8/JNK1. It is required for histone H4 acetylation DSBs and its ability to specifically bind modified histones and chromatin modifying enzymes such as KAT5/TIP60, probably explains its trancription activation activity. It functions in association with TSHZ3, SET and HDAC factors as a transcriptional repressor that inhibits the expression of CASP4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: APBB1