GKAP/DLGAP1 Antibody

Images

 
Western Blot: GKAP/DLGAP1 Antibody [NBP1-76911] - Analysis in rat brain tissue lysate with antibody at (A) 0.5 and (B) 1 ug/mL.
Immunocytochemistry/ Immunofluorescence: GKAP/DLGAP1 Antibody [NBP1-76911] - Human Brain cells
Immunohistochemistry-Paraffin: GKAP/DLGAP1 Antibody [NBP1-76911] - Human brain with DLGAP1 antibody at 5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

GKAP/DLGAP1 Antibody Summary

Immunogen
Antibody was raised against a 18 amino acid synthetic peptide from near the center of human SAPAP1. The immunogen is located within amino acids 640 - 690 of SAPAP1.
Specificity
At least three isoforms of SAPAP1 are known to exist. This SAPAP1 antibody will not cross-react with other SAPAP proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DLGAP1
Purity
Peptide affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/mL
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Control Peptide

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for GKAP/DLGAP1 Antibody

  • DAP1
  • DAP-1-ALPHA
  • DAP-1DAP-1-BETA
  • discs, large (Drosophila) homolog-associated protein 1
  • disks large-associated protein 1
  • DLGAP1
  • GKAP
  • GKAPMGC88156
  • hGKAP
  • PSD-95/SAP90 binding protein 1
  • PSD-95/SAP90-binding protein 1
  • SAP90/PSD-95-associated protein 1
  • SAPAP1
  • SAPAP1Guanylate kinase-associated protein

Background

SAP90/PSD-95-associated protein 1 (SAPAP1, also known as DLGAP1 or GKAP) is a member of a protein family whose members specifically interact with PSD-95/SAP90, a membrane-associated guanylate kinase localized at postsynaptic density (PSD) in neuronal cells. Like the other SAPAP proteins, SAPAP1 is thought to be an adaptor protein that also interacts with different synaptic scaffolding proteins, cytoskeletal and signaling components, such as focal adhesion kinase (FAK) and proline-rich tyrosine kinase 2 (PYK2). SAPAP1 mRNA is targeted to cell bodies in a similar manner to SAPAP2 and -4, whereas SAPAP3 mRNA is detected mainly in cell bodies. SAPAP1 protein however, is targeted to the synapse and is not reliant on the synaptic localization of PSD-95 or the synaptic scaffolding molecule (S-SCAM). At least three isoforms of SAPAP1 are known to exist. This SAPAP1 antibody will not cross-react with other SAPAP proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol DLGAP1
Uniprot