TRIAD3 Antibody - BSA Free

Immunoprecipitation:[NBP3-29793]-Detection of human RNF216 by western blot of immunoprecipitates. Samples: Whole cell lysate from HeLa cells. Antibodies: Affinity purified rabbit anti-RNF216 antibody used for IP at 6 µg/mg lysate. RNF216 was also immunoprecipitated by rabbit anti-RNF216 antibodies , and. For blotting immunoprecipitated RNF216, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TRIAD3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TRIAD3 Antibody - BSA Free (NBP3-29793) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Between 100 and 150
• Assay Type: [Assay Type]
• Sample Volume: [Sample Volume]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RNF216
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-citrate/phosphate buffer, pH 7 to 8
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Alternate Names for TRIAD3 Antibody - BSA Free

• EC 6.3.2.-
• ring finger protein 216Ubiquitin-conjugating enzyme 7-interacting protein 1
• Triad domain-containing protein 3
• TRIAD3Zinc finger protein inhibiting NF-kappa-B
• U7I1
• UBCE7IP1ubiquitin conjugating enzyme 7 interacting protein 1
• ZINE3 ubiquitin-protein ligase RNF216

Background

The TRIAD3 gene encodes a cytoplasmic protein which specifically colocalizes and interacts with the serine/threonine protein kinase, receptor-interacting protein (RIP). Zinc finger domains of the encoded protein are required for its interaction with RIP and for inhibition of TNF- and IL1-induced NF-kappa B activation pathways. The encoded protein may also function as an E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes and transfers it to substrates. Several alternatively spliced transcript variants have been described for this locus but the full-length natures of only some are known.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RNF216