TRIAD3 Antibody - BSA Free

Western Blot: TRIAD3 Antibody [NBP1-76592] - Analysis of TRIAD3 in mouse heart tissue lysate with TRIAD3 antibody at (A) 1 and (B) 2 ug/mL.

Immunocytochemistry/ Immunofluorescence: TRIAD3 Antibody - BSA Free [NBP1-76592] - Immunofluorescence of TRIAD3 in Mouse Heart cells with TRIAD3 antibody at 20 ug/mL.

Immunohistochemistry: TRIAD3 Antibody - BSA Free [NBP1-76592] - Immunohistochemistry of TRIAD3 in mouse heart with TRIAD3 antibody at 10 u/mL.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

TRIAD3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TRIAD3 Antibody - BSA Free (NBP1-76592) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a peptide corresponding to 15 amino acids near the amino-terminus of mouse TRIAD3A. The immunogen is located within amino acids 120 - 170 of TRIAD3A. Amino Acid Squence: TESDPLETQNQSSED
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RNF216
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/mL
  • Western Blot 1-2 ug/ml
• Theoretical MW: 95 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for TRIAD3 Antibody - BSA Free

• EC 6.3.2.-
• ring finger protein 216Ubiquitin-conjugating enzyme 7-interacting protein 1
• Triad domain-containing protein 3
• TRIAD3Zinc finger protein inhibiting NF-kappa-B
• U7I1
• UBCE7IP1ubiquitin conjugating enzyme 7 interacting protein 1
• ZINE3 ubiquitin-protein ligase RNF216

Background

Activation of NF-kappaB as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses. Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88. Several regulatory mechanisms exist to control TLR signal transduction, including the inhibition of TLR expression and signaling by molecules such as ST2 and SIGIRR. Another mechanism is by the ubi-quitinization of selected TLRs by TRIAD3A, an E3 ubiquitin-protein ligase. TRIAD3A is a RING finger protein that can bind to TLR4 and TLR9, and to a lesser extent TLR3 and TLR5, catalyzing the ubiquitization of these molecules. Overexpression of TRIAD3A promoted the nearly complete degradation of TLR4 and TLR9; this reduction was reflected in the decreased signal-specific activation by ligands specific for these TLRs. Conversely, depletion of TRIAD3A resulted in enhanced TLR activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RNF216
• Uniprot:
  • AAP47174(Human)