TREM2 Antibody [Biotin]

TREM-2 was detected in immersion fixed mouse spleno-cytes using Mouse Anti-TREM-2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1729) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Recombinant Human/Mouse TREM2 protein was serially diluted 2-fold and captured by Rat Anti-Human/Mouse TREM2 Monoclonal Antibody (Catalog # MAB17291) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Mouse TREM2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1729) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Recombinant Mouse TREM2 protein was serially diluted 2-fold and captured by Sheep Anti-Mouse TREM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1729) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Mouse TREM2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1729) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

• Reactivity: Mu
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Biotin
• Concentration: LYOPH

TREM2 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse TREM2b
  Leu19-Pro168
  Accession # Q99NH8
• Specificity: Detects mouse TREM2 in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human (rh) TREM2 is observed and less than 1% cross-reactivity with recombinant mouse (rm) TREM1 and rmTREM3 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: TREM2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TREM2 Antibody [Biotin]

• PLOSL2
• TREM2
• TREM-2
• Trem2a
• Trem2b
• Trem2c
• TREM-2triggering receptor expressed on myeloid cells 2a
• Triggering receptor expressed on monocytes 2
• triggering receptor expressed on myeloid cells 2

Background

TREM2 (Triggering Receptor Expressed by Myeloid cells) is an Ig superfamily cell surface receptor that activates a number of myeloid cell types (1). It is a member of a small gene family located on human chromosome 6p21 and mouse chromosome 17 in a region linked to the MHC (2). A single human TREM2 gene has been described, however, two closely related orthologs were reported in mouse (3). The proteins differ by only three amino acids and were designated TREM2a and TREM2b. TREM2 is type I transmembrane protein consisting of a single extracellular immunoglobulin (V-like) domain, a transmembrane domain with a positively charged lysine residue, and a short cytoplasmic tail (1). It associates with the signal adapter protein, DAP12, for signaling and function. DAP12 has a cytoplasmic ITAM that is phosphorylated upon ligand binding leading to the subsequent activation of cytoplasmic tyrosine kinases. TREM2 is expressed by immature monocyte-derived dendritic cells (DC), and expression is down-regulated upon activation of DC by microbial products and costimulatory signals (4). Ligation of TREM2 on immature DC with anti-TREM2 antibodies results in partial DC activation and the up-regulation of CCR7 and some co-stimulatory molecules. A role for TREM2 in the functioning of osteoclasts and microglia is suggested by the discovery that homozygous loss-of-function mutations in either TREM2 or DAP12 result in Nasu-Hakola disease characterized by a combination of presenile demetia and bone cysts (5). In vitro studies indicate that the differentiation of myeloid precursors into osteoclasts is dramatically impaired in TREM2 deficient individuals (6).

1. Colonna, M. (2003) Nature Rev. Immunol. 3:445.
2. Allcock, R. et al. (2003) Eur. J. Immunol. 33:567.
3. Daws, M. et al. (2001) Eur. J. Immunol. 31:783.
4. Bouchon, A. et al. (2001) J. Exp. Med. 194:1111.
5. Paloneva, J. et al. (2002) Am. J. Hum. Genet. 71:656.
6. Cella, M. et al. (2003) J. Exp. Med. 198:645.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TREM2 Antibody (BAF1729)(25)

Publications using BAF1729

• de Weerd, L;Hummel, S;Müller, SA;Paris, I;Sandmann, T;Eichholtz, M;Gröger, R;Englert, AL;Wagner, S;Ha, C;Davis, SS;Warkins, V;Xia, D;Nuscher, B;Berghofer, A;Reich, M;Feiten, AF;Schlepckow, K;Willem, M;Lichtenthaler, SF;Lewcock, JW;Monroe, KM;Brendel, M;Haass, C; Early intervention anti-A? immunotherapy attenuates microglial activation without inducing exhaustion at residual plaques Molecular neurodegeneration 2025-08-20 [PMID: 40830489] (ELISA Capture, Mouse)
• Takalo, M;Jeskanen, H;Rolova, T;Kervinen, I;Hellén, M;Heikkinen, S;Koivisto, H;Jokivarsi, K;Müller, SA;Koivumäki, EM;Mäkinen, P;Juopperi, SP;Willman, RM;Sinisalo, R;Hoffmann, D;Jäntti, H;Peitz, M;Flie beta bach, K;Kuulasmaa, T;Natunen, T;Kemppainen, S;Poutiainen, P;Leinonen, V;Malm, T;Martiskainen, H;Ramirez, A;Haapasalo, A;Lichtenthaler, SF;Tanila, H;Haass, C;Rinne, J;Koistinaho, J;Hiltunen, M; The protective PLC?2-P522R variant mitigates Alzheimer's disease-associated pathologies by enhancing beneficial microglial functions Journal of neuroinflammation 2025-03-05 [PMID: 40038760] (ELISA Detection, Mouse)
• Pierro, EW;Cottam, MA;An, H;Lehmann, BD;Pietenpol, JA;Wellen, KE;Makowski, L;Rathmell, JC;Fingleton, B;Hasty, AH; Trem2 deficiency attenuates breast cancer tumor growth in lean, but not obese or weight loss, mice and is associated with alterations of clonal T cell populations bioRxiv : the preprint server for biology 2024-09-27 [PMID: 39386686] (ELISA Detection, Mouse)
• Yin, T;D'Adamio, L; BRI2-mediated regulation of TREM2 processing in microglia and its potential implications for Alzheimer's disease and related dementias bioRxiv : the preprint server for biology 2023-06-14 [PMID: 37398330] (ELISA Capture, Mouse, N/A, Transgenic Mouse)
• X Shu, C Wei, WY Tu, K Zhong, S Qi, A Wang, L Bai, SX Zhang, B Luo, ZZ Xu, K Zhang, C Shen Negative regulation of TREM2-mediated C9orf72 poly-GA clearance by the NLRP3 inflammasome Cell Reports, 2023-02-16;42(2):112133. 2023-02-16 [PMID: 36800288] (ELISA Detection, Mouse)
• SF You, L Brase, F Filipello, AK Iyer, J Del-Aguila, J He, R D'Oliveira, J Budde, J Norton, J Gentsch, NM Dräger, SM Sattler, M Kampmann, L Piccio, JC Morris, RJ Perrin, E McDade, Dominantly, SM Paul, AG Cashikar, BA Benitez, O Harari, CM Karch MS4A4A modifies the risk of Alzheimer disease by regulating lipid metabolism and immune response in a unique microglia state medRxiv : the preprint server for health sciences, 2023-02-08;0(0):. 2023-02-08 [PMID: 36798226] (ELISA Capture, Mouse)
• Wenhui Qiao, Yixing Chen, Jun Zhong, Benjamin J. Madden, Cristine M. Charlesworth, Yuka A. Martens et al. Trem2 H157Y increases soluble TREM2 production and reduces amyloid pathology Molecular Neurodegeneration 2023-01-31 [PMID: 36721205]
• C Rother, RE Uhlmann, SA Müller, J Schelle, A Skodras, U Obermüller, LM Häsler, M Lambert, F Baumann, Y Xu, C Bergmann, G Salvadori, M Loos, I Brzak, D Shimshek, U Neumann, Dominantly, LC Walker, SA Schultz, JP Chhatwal, SA Kaeser, SF Lichtentha, M Staufenbie, M Jucker Experimental evidence for temporal uncoupling of brain Abeta deposition and neurodegenerative sequelae Nature Communications, 2022-11-28;13(1):7333. 2022-11-28 [PMID: 36443293] (ELISA Capture, Mouse)
• S Roshanbin, U Julku, M Xiong, J Eriksson, E Masliah, G Hultqvist, J Bergström, M Ingelsson, S Syvänen, D Sehlin Reduction of alphaSYN Pathology in a Mouse Model of PD Using a Brain-Penetrating Bispecific Antibody Pharmaceutics, 2022-07-05;14(7):. 2022-07-05 [PMID: 35890306] (ELISA Detection, Mouse)
• D Xia, S Lianoglou, T Sandmann, M Calvert, JH Suh, E Thomsen, J Dugas, ME Pizzo, SL DeVos, TK Earr, CC Lin, S Davis, C Ha, AW Leung, H Nguyen, R Chau, E Yulyanings, I Lopez, H Solanoy, ST Masoud, CC Liang, K Lin, G Astarita, N Khoury, JY Zuchero, RG Thorne, K Shen, S Miller, JJ Palop, D Garceau, M Sasner, JD Whitesell, JA Harris, S Hummel, J Gnörich, K Wind, L Kunze, A Zatcepin, M Brendel, M Willem, C Haass, D Barnett, TS Zimmer, AG Orr, K Scearce-Le, JW Lewcock, G Di Paolo, PE Sanchez Novel App knock-in mouse model shows key features of amyloid pathology and reveals profound metabolic dysregulation of microglia Molecular Neurodegeneration, 2022-06-11;17(1):41. 2022-06-11 [PMID: 35690868] (ELISA Capture, Mouse)
• Logan T, Simon MJ, Rana A Et al. Rescue of a lysosomal storage disorder caused by Grn loss of function with a brain penetrant progranulin biologic Cell 8/25/2021 [PMID: 34450028]
• Y Huang, KE Happonen, PG Burrola, C O'Connor, N Hah, L Huang, A Nimmerjahn, G Lemke Microglia use TAM receptors to detect and engulf amyloid &beta plaques Nature Immunology, 2021-04-15;0(0):. 2021-04-15 [PMID: 33859405] (IHC, Mouse)
• G Biechele, K Wind, T Blume, C Sacher, L Beyer, F Eckenweber, N Franzmeier, M Ewers, B Zott, S Lindner, FJ Gildehaus, B von Ungern, S Tahirovic, M Willem, P Bartenstei, P Cumming, A Rominger, J Herms, M Brendel Microglial Activation in the Right Amygdala-Entorhinal-Hippocampal Complex is Associated with Preserved Spatial Learning in AppNL-G-F mice Neuroimage, 2020-12-29;0(0):117707. 2020-12-29 [PMID: 33385560] (ELISA Development, Mouse)
• VS Jadhav, PBC Lin, T Pennington, GV Di Prisco, AJ Jannu, G Xu, M Moutinho, J Zhang, BK Atwood, SS Puntambeka, SJ Bissel, AL Oblak, GE Landreth, BT Lamb Trem2 Y38C mutation and loss of Trem2 impairs neuronal synapses in adult mice Mol Neurodegener, 2020-10-28;15(1):62. 2020-10-28 [PMID: 33115519] (ELISA Detection, Mouse)
• Wenfei Liu, Orjona Taso, Rui Wang, Sevinc Bayram, Andrew C Graham, Pablo Garcia-Reitboeck et al. Trem2 promotes anti-inflammatory responses in microglia and is suppressed under pro-inflammatory conditions Human Molecular Genetics 2020-11-25 [PMID: 32959884]
• K Schlepckow, KM Monroe, G Kleinberge, L Cantuti-Ca, S Parhizkar, D Xia, M Willem, G Werner, N Pettkus, B Brunner, A Sülzen, B Nuscher, H Hampel, X Xiang, R Feederle, S Tahirovic, JI Park, R Prorok, C Mahon, CC Liang, J Shi, DJ Kim, H Sabelström, F Huang, G Di Paolo, M Simons, JW Lewcock, C Haass Enhancing protective microglial activities with a dual function TREM2 antibody to the stalk region EMBO Mol Med, 2020-03-10;0(0):e11227. 2020-03-10 [PMID: 32154671] (ELISA Capture, Mouse)
• Erin G. Reed-Geaghan, Andrew L. Croxford, Burkhard Becher, Gary E. Landreth Plaque-associated myeloid cells derive from resident microglia in an Alzheimer’s disease model Journal of Experimental Medicine 2020-04-06 [PMID: 31967645]
• Christian Sacher, Tanja Blume, Leonie Beyer, Finn Peters, Florian Eckenweber, Carmelo Sgobio et al. Longitudinal PET Monitoring of Amyloidosis and Microglial Activation in a Second-Generation Amyloid-beta Mouse Model Journal of Nuclear Medicine 2019-12-01 [PMID: 31302633]
• JK Götzl, M Brendel, G Werner, S Parhizkar, L Sebastian, G Kleinberge, AV Colombo, M Deussing, M Wagner, J Winkelmann, J Diehl-Schm, J Levin, K Fellerer, A Reifschnei, S Bultmann, P Bartenstei, A Rominger, S Tahirovic, ST Smith, C Madore, O Butovsky, A Capell, C Haass Opposite microglial activation stages upon loss of PGRN or TREM2 result in reduced cerebral glucose metabolism EMBO Mol Med, 2019-06-01;0(0):. 2019-06-01 [PMID: 31122931] (ELISA Capture, Mouse)
• L Zhong, Y Xu, R Zhuo, T Wang, K Wang, R Huang, D Wang, Y Gao, Y Zhu, X Sheng, K Chen, N Wang, L Zhu, D Can, Y Marten, M Shinohara, CC Liu, D Du, H Sun, L Wen, H Xu, G Bu, XF Chen Soluble TREM2 ameliorates pathological phenotypes by modulating microglial functions in an Alzheimer's disease model Nat Commun, 2019-03-25;10(1):1365. 2019-03-25 [PMID: 30911003] (ELISA Development (Detection), Mouse)
• G Kleinberge, M Brendel, E Mracsko, B Wefers, L Groeneweg, X Xiang, C Focke, M Deu beta ing, M Suárez-Cal, F Mazaheri, S Parhizkar, N Pettkus, W Wurst, R Feederle, P Bartenstei, T Mueggler, T Arzberger, I Knuesel, A Rominger, C Haass The FTD-like syndrome causing TREM2 T66M mutation impairs microglia function, brain perfusion, and glucose metabolism EMBO J., 2017-05-30;0(0):. 2017-05-30 [PMID: 28559417] (ELISA Development, Immunoprecipitation, Mouse)
• M Brendel, G Kleinberge, F Probst, A Jaworska, F Overhoff, T Blume, NL Albert, J Carlsen, S Lindner, FJ Gildehaus, L Ozmen, M Suárez-Cal, P Bartenstei, K Baumann, M Ewers, J Herms, C Haass, A Rominger Increase of TREM2 during Aging of an Alzheimer's Disease Mouse Model Is Paralleled by Microglial Activation and Amyloidosis Front Aging Neurosci, 2017-01-31;9(0):8. 2017-01-31 [PMID: 28197095] (ELISA Development (Capture), Western Blot, Mouse)
• Sharif O, Gawish R, Warszawska J, Martins R, Lakovits K, Hladik A, Doninger B, Brunner J, Korosec A, Schwarzenbacher R, Berg T, Kralovics R, Colinge J, Mesteri I, Gilfillan S, Salmaggi A, Verschoor A, Colonna M, Knapp S The triggering receptor expressed on myeloid cells 2 inhibits complement component 1q effector mechanisms and exerts detrimental effects during pneumococcal pneumonia. PLoS Pathog, 2014-06-12;10(6):e1004167. 2014-06-12 [PMID: 24945405] (Western Blot, Mouse)
• Masahito Kawabori, Masaaki Hokari, Zhen Zheng, Jong Youl Kim, Cyrus Calosing, Christine L. Hsieh et al. Triggering Receptor Expressed on Myeloid Cells-2 Correlates to Hypothermic Neuroprotection in Ischemic Stroke Therapeutic Hypothermia and Temperature Management 2013-12-01 [PMID: 24380032]
• Koth LL, Cambier CJ, Ellwanger A DAP12 is required for macrophage recruitment to the lung in response to cigarette smoke and chemotaxis toward CCL2. J. Immunol., 2010-04-26;184(11):6522-8. 2010-04-26 [PMID: 20421649] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: TREM2
• Uniprot:
  • Q99NH8(Mouse)