NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="TREM2 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Sheep Anti-Mouse TREM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1729) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

DY990). Sheep Anti-Mouse TREM2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (BAF1729) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_lightbox"> DY990). Sheep Anti-Mouse TREM2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (BAF1729) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_thumb" />

NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

MAB0061, open histogram), followed by APC-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (F0113)." class="big_lightbox"> MAB0061, open histogram), followed by APC-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (F0113)." alt="RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Human/Mouse TREM2 Monoclonal Antibody (Catalog # MAB17291, filled histogram) or isotype control antibody (MAB0061, open histogram), followed by APC-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (F0113)." class="big_thumb" />

MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (F0113)." class="big_lightbox"> MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (F0113)." class="big_thumb" />

MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (F0113)." class="big_lightbox"> MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (F0113)." class="big_thumb" />

F0113). Quadrant markers were set based on control antibody staining (MAB0061)." class="big_lightbox"> F0113). Quadrant markers were set based on control antibody staining (MAB0061)." class="big_thumb" />

NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.Staining was performed using our Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.Staining was performed using our Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

DY990). Sheep Anti-Mouse TREM2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (BAF1729) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_lightbox"> DY990). Sheep Anti-Mouse TREM2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (BAF1729) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_thumb" />

MAB0061) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox"> MAB0061) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

MAB18281) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human TREM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF18281) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_lightbox"> MAB18281) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human TREM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF18281) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." alt="Recombinant Human TREM2 protein was serially diluted 2-fold and captured by Mouse Anti-Human TREM2 Monoclonal Antibody (MAB18281) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human TREM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF18281) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_thumb" />