TREM2 Antibodies

NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="TREM2 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Sheep Anti-Mouse TREM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1729) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

DY990). Sheep Anti-Mouse TREM2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (BAF1729) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_lightbox"> DY990). Sheep Anti-Mouse TREM2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (BAF1729) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_thumb" />

NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

MAB0061, open histogram), followed by APC-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (F0113)." class="big_lightbox"> MAB0061, open histogram), followed by APC-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (F0113)." alt="RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Human/Mouse TREM2 Monoclonal Antibody (Catalog # MAB17291, filled histogram) or isotype control antibody (MAB0061, open histogram), followed by APC-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (F0113)." class="big_thumb" />

MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (F0113)." class="big_lightbox"> MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (F0113)." class="big_thumb" />

MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (F0113)." class="big_lightbox"> MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (F0113)." class="big_thumb" />

F0113). Quadrant markers were set based on control antibody staining (MAB0061)." class="big_lightbox"> F0113). Quadrant markers were set based on control antibody staining (MAB0061)." class="big_thumb" />

NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.Staining was performed using our Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.Staining was performed using our Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

DY990). Sheep Anti-Mouse TREM2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (BAF1729) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_lightbox"> DY990). Sheep Anti-Mouse TREM2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (BAF1729) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_thumb" />

MAB0061) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox"> MAB0061) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

MAB18281) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human TREM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF18281) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_lightbox"> MAB18281) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human TREM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF18281) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." alt="Recombinant Human TREM2 protein was serially diluted 2-fold and captured by Mouse Anti-Human TREM2 Monoclonal Antibody (MAB18281) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human TREM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF18281) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_thumb" />

VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="TREM2 was detected in immersion fixed paraffin-embedded sections of human lung using Rabbit Anti-Human TREM2 Monoclonal Antibody (Catalog # MAB11618) at 3 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of microglia. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of microglia. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (F0105B). Staining was performed using our Staining Membrane-Associated Proteins protocol." class="big_lightbox"> MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (F0105B). Staining was performed using our Staining Membrane-Associated Proteins protocol." alt="RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Human/Mouse TREM2 Monoclonal Antibody (Catalog # MAB17291R, filled histogram) or isotype control antibody (MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (F0105B). Staining was performed using our Staining Membrane-Associated Proteins protocol." class="big_thumb" />

MAB0061) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (F0113) and Rat anti-Mouse Integrin alpha M/CD11b PE-conjugated Monoclonal Antibody (FAB1124P). Staining was performed using our Staining Membrane-Associated Proteins protocol. " class="big_lightbox"> MAB0061) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (F0113) and Rat anti-Mouse Integrin alpha M/CD11b PE-conjugated Monoclonal Antibody (FAB1124P). Staining was performed using our Staining Membrane-Associated Proteins protocol. " class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="TREM2 was detected in immersion fixed paraffin-embedded sections of Human Brain Medulla (Locus Coeruleus) using Mouse Anti-Human TREM2 Monoclonal Antibody (Catalog # MAB18282) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />