RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Human/Mouse TREM2 Monoclonal Antibody (Catalog # MAB17291, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), ...read more
RecombinantMouse TREM2 protein was serially diluted 2-fold and captured by Rat Anti-Human/Mouse TREM2 Monoclonal Antibody (Catalog # MAB17291) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep ...read more
THP‑1 human acute monocytic leukemia cell line was stained with Rat Anti-Human/Mouse TREM2 Monoclonal Antibody (Catalog # MAB17291, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), ...read more
Human mature dendritic cells were stained with Rat Anti-Human/Mouse TREM2 Monoclonal Antibody (Catalog # MAB17291, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by ...read more
HEK293 human embryonic kidney cell line transfected with eGFP and either (A) human TREM2 or (B) human TREM1 was stained with Rat Anti-Human/Mouse TREM2 Monoclonal Antibody (Catalog # MAB17291) followed by ...read more
Detects human and mouse TREM2 in direct ELISAs. Stains TREM2 transfectants but not TREM1 transfectants in flow cytometry. In sandwich ELISAs, detects mouse TREM2 when paired with a mouse TREM2 detection antibody.
Protein A or G purified from hybridoma culture supernatant
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.5 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for TREM2 Antibody (237920) [Unconjugated]
TREM-2triggering receptor expressed on myeloid cells 2a
Triggering receptor expressed on monocytes 2
triggering receptor expressed on myeloid cells 2
TREM2 (Triggering Receptor Expressed on Myeloid cells-2) is
a 35 kDa molecular weight type I transmembrane member of the TREM family and Ig
superfamily . Mature human TREM2 consists of a 156 amino acid (aa)
extracellular domain (ECD) with one V-type Ig-like domain, a 21 aa
transmembrane (TM) domain, and a 35 aa cytoplasmic tail. Within the ECD, human
TREM2 shares 73% and 74% aa sequence identity with mouse and rat TREM2,
respectively. Two closely related transcripts were reported in mouse and
designated TREM2a and TREM2b. Soluble forms of the TREM2 ECD are generated
by alternative splicing or proteolytic cleavage, and the cytoplasmic domain can
be liberated by gamma-Secretase mediated intramembrane cleavage. It is a pattern
recognition receptor that binds anionic ligands. A positively charged lysine
within the transmembrane segment allows association with the signal adapter
protein, DAP12 to deliver an activating signal that plays a role in both innate
and adaptive immune responses, including inhibition of macrophage activation.
TREM2 is expressed on macrophages, immature myeloid dendritic cells,
osteoclasts, microglia, and adipocytes. It promotes the differentiation and
function of osteoclasts, the production of inflammatory cytokines by
adipocytes, insulin resistance, and the phagocytic clearance of bacteria. In
the CNS, TREM2 binds to ApoE, ApoA1, and ApoB and mediates the clearance of
apoptotic neurons, amyloid plaques, and cell debris following demyelination.
TREM2 also interacts with and modifies signaling through Plexin A1 on
dendritic cells and osteoclasts. Mutations in TREM2 or DAP12 are associated
with the development of Alzheimer's disease and Nasu-Hakola disease (NHD/PLOSL)
which is characterized by presenile dementia and bone cysts. Soluble TREM2 is
elevated in cerebrospinal fluid of patients with active multiple sclerosis
(MS), and TREM2 blockade exacerbates disease symptoms in the experimental EAE
model of MS.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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