TREM2 Antibody (237920) [Unconjugated]

Images

 
RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Human/Mouse TREM2 Monoclonal Antibody (Catalog # MAB17291, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), ...read more
RecombinantMouse TREM2 protein was serially diluted 2-fold and captured by Rat Anti-Human/Mouse TREM2 Monoclonal Antibody (Catalog # MAB17291) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep ...read more
THP‑1 human acute monocytic leukemia cell line was stained with Rat Anti-Human/Mouse TREM2 Monoclonal Antibody (Catalog # MAB17291, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), ...read more
Human mature dendritic cells were stained with Rat Anti-Human/Mouse TREM2 Monoclonal Antibody (Catalog # MAB17291, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by ...read more
HEK293 human embryonic kidney cell line transfected with eGFP and either (A) human TREM2 or (B) human TREM1 was stained with Rat Anti-Human/Mouse TREM2 Monoclonal Antibody (Catalog # MAB17291) followed by ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications ELISA, Flow, CyTOF-ready, ICC/IF, KO
Clone
237920
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

TREM2 Antibody (237920) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TREM2
extracellular domain
Accession # Q99NH8
Specificity
Detects human and mouse TREM2 in direct ELISAs. Stains TREM2 transfectants but not TREM1 transfectants in flow cytometry. In sandwich ELISAs, detects mouse TREM2 when paired with a mouse TREM2 detection antibody.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
TREM2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 5-25 ug/mL
  • Knockout Validated
Publications
Read Publications using
MAB17291 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TREM2 Antibody (237920) [Unconjugated]

  • PLOSL2
  • TREM2
  • TREM-2
  • Trem2a
  • Trem2b
  • Trem2c
  • TREM-2triggering receptor expressed on myeloid cells 2a
  • Triggering receptor expressed on monocytes 2
  • triggering receptor expressed on myeloid cells 2

Background

TREM2 (Triggering Receptor Expressed on Myeloid cells-2) is a 35 kDa molecular weight type I transmembrane member of the TREM family and Ig superfamily . Mature human TREM2 consists of a 156 amino acid (aa) extracellular domain (ECD) with one V-type Ig-like domain, a 21 aa transmembrane (TM) domain, and a 35 aa cytoplasmic tail. Within the ECD, human TREM2 shares 73% and 74% aa sequence identity with mouse and rat TREM2, respectively. Two closely related transcripts were reported in mouse and designated TREM2a and TREM2b. Soluble forms of the TREM2 ECD are generated by alternative splicing or proteolytic cleavage, and the cytoplasmic domain can be liberated by gamma-Secretase mediated intramembrane cleavage. It is a pattern recognition receptor that binds anionic ligands. A positively charged lysine within the transmembrane segment allows association with the signal adapter protein, DAP12 to deliver an activating signal that plays a role in both innate and adaptive immune responses, including inhibition of macrophage activation. TREM2 is expressed on macrophages, immature myeloid dendritic cells, osteoclasts, microglia, and adipocytes. It promotes the differentiation and function of osteoclasts, the production of inflammatory cytokines by adipocytes, insulin resistance, and the phagocytic clearance of bacteria. In the CNS, TREM2 binds to ApoE, ApoA1, and ApoB and mediates the clearance of apoptotic neurons, amyloid plaques, and cell debris following demyelination. TREM2 also interacts with and modifies signaling through Plexin A1 on dendritic cells and osteoclasts. Mutations in TREM2 or DAP12 are associated with the development of Alzheimer's disease and Nasu-Hakola disease (NHD/PLOSL) which is characterized by presenile dementia and bone cysts. Soluble TREM2 is elevated in cerebrospinal fluid of patients with active multiple sclerosis (MS), and TREM2 blockade exacerbates disease symptoms in the experimental EAE model of MS.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TREM2 Antibody (MAB17291)(14)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 8 applications: CyTOF, ELISA Detection, ELISA Development (Capture), Flow Cytometry, Functional Assay, ICC, IHC, Western Blot.


Filter By Application
CyTOF
(1)
ELISA Detection
(1)
ELISA Development (Capture)
(1)
Flow Cytometry
(3)
Functional Assay
(2)
ICC
(1)
IHC
(2)
Western Blot
(2)
All Applications
Filter By Species
Human
(5)
Mouse
(8)
Rat
(1)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using MAB17291 Applications Species
K Cosker, A Mallach, J Limaye, TM Piers, J Staddon, SJ Neame, J Hardy, JM Pocock Microglial? signalling pathway deficits associated with the patient derived R47H TREM2 variants linked to AD indicate inability to activate inflammasome Scientific Reports, 2021;11(1):13316. 2021 [PMID: 34172778] (Functional Assay, Human) Functional Assay Human
C Munn, S Burton, S Dickerson, K Bakshy, A Strouse, D Rajesh Generation of cryopreserved macrophages from normal and genetically engineered human pluripotent stem cells for disease modelling PLoS ONE, 2021;16(4):e0250107. 2021 [PMID: 33886609] (ICC, Human) ICC Human
HR Chen, CW Chen, N Mandhani, JC Short-Mill, MR Smucker, YY Sun, CY Kuan Monocytic Infiltrates Contribute to Autistic-like Behaviors in a Two-Hit Model of Neurodevelopmental Defects J Neurosci, 2020;0(0):. 2020 [PMID: 33127853] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Y Wang, Y Shi, Y Huang, W Liu, G Cai, S Huang, Y Zeng, S Ren, H Zhan, W Wu Resveratrol mediates mechanical allodynia through modulating inflammatory response via the TREM2-autophagy axis in SNI rat model J Neuroinflammation, 2020;17(1):311. 2020 [PMID: 33081801] (Western Blot, Rat) Western Blot Rat
BL Heckmann, BJW Teubner, E Boada-Rome, B Tummers, C Guy, P Fitzgerald, U Mayer, S Carding, SS Zakharenko, T Wileman, DR Green Noncanonical function of an autophagy protein prevents spontaneous Alzheimer's disease Sci Adv, 2020;6(33):eabb9036. 2020 [PMID: 32851186] (Western Blot, Mouse) Western Blot Mouse
JK Götzl, M Brendel, G Werner, S Parhizkar, L Sebastian, G Kleinberge, AV Colombo, M Deussing, M Wagner, J Winkelmann, J Diehl-Schm, J Levin, K Fellerer, A Reifschnei, S Bultmann, P Bartenstei, A Rominger, S Tahirovic, ST Smith, C Madore, O Butovsky, A Capell, C Haass Opposite microglial activation stages upon loss of PGRN or TREM2 result in reduced cerebral glucose metabolism EMBO Mol Med, 2019;0(0):. 2019 [PMID: 31122931] (ELISA Detection, Mouse) ELISA Detection Mouse
L Zhong, Y Xu, R Zhuo, T Wang, K Wang, R Huang, D Wang, Y Gao, Y Zhu, X Sheng, K Chen, N Wang, L Zhu, D Can, Y Marten, M Shinohara, CC Liu, D Du, H Sun, L Wen, H Xu, G Bu, XF Chen Soluble TREM2 ameliorates pathological phenotypes by modulating microglial functions in an Alzheimer's disease model Nat Commun, 2019;10(1):1365. 2019 [PMID: 30911003] (ELISA Development (Capture), Mouse) ELISA Development (Capture) Mouse
MA Russo, NT Fiore, C van Vreden, D Bailey, DM Santarelli, HM McGuire, B Fazekas de, PJ Austin Expansion and activation of distinct central memory T lymphocyte subsets in complex regional pain syndrome J Neuroinflammation, 2019;16(1):63. 2019 [PMID: 30885223] (CyTOF, Human) CyTOF Human
P Garcia-Rei, A Phillips, TM Piers, C Villegas-L, M Butler, A Mallach, C Rodrigues, CE Arber, A Heslegrave, H Zetterberg, H Neumann, S Neame, H Houlden, J Hardy, JM Pocock Human Induced Pluripotent Stem Cell-Derived Microglia-Like Cells Harboring TREM2 Missense Mutations Show Specific Deficits in Phagocytosis Cell Rep, 2018;24(9):2300-2311. 2018 [PMID: 30157425] (Human) Human
MM Varnum, KA Clayton, A Yoshii-Kit, G Yonemoto, L Koro, S Ikezu, T Ikezu A split-luciferase complementation, real-time reporting assay enables monitoring of the disease-associated transmembrane protein TREM2 in live cells J. Biol. Chem., 2017;0(0):. 2017 [PMID: 28490631] (Functional Assay, Human) Functional Assay Human
Show All 14 Publications.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB17291U
Alexa Fluor 405 FAB17291V
Alexa Fluor 488 FAB17291G
Alexa Fluor 532 FAB17291X
Alexa Fluor 594 FAB17291T
Alexa Fluor 594 FAB17291T
Alexa Fluor 647 FAB17291R
Alexa Fluor 700 FAB17291N
Alexa Fluor 750 FAB17291S
Allophycocyanin FAB17291A
Biotin FAB17291B
DyLight 350 FAB17291D
DyLight 405 FAB17291E
DyLight 488 FAB17291K
DyLight 550 FAB17291L
DyLight 594 FAB17291M
DyLight 650 FAB17291W
DyLight 680 FAB17291Y
DyLight 755 FAB17291Z
FITC FAB17291F
HRP FAB17291H
Janelia Fluor 549 FAB17291I
Janelia Fluor 646 FAB17291J
PerCP (Peridinin-chlorophyll Protein Complex) FAB17291C
Phycoerythrin FAB17291P

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Bioinformatics

Gene Symbol TREM2
Uniprot