NCOA6 Antibody


Western Blot: NCOA6 Antibody [NB200-335] - Detection of Human ASC2 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit more
Immunohistochemistry-Paraffin: NCOA6 Antibody [NB200-335] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-ASC2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: NCOA6 Antibody [NB200-335] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ASC2 more

Product Details

Reactivity Hu, RtSpecies Glossary
Applications WB, IHC, IP
1.0 mg/ml

NCOA6 Antibody Summary

A synthetic peptide that maps to a region between residues 1050 and 1100 of human Activating Signal Cointegrator-2 using the numbering given in Swiss-Prot entry Q14686 (GeneID 23054).
Predicted Species
Rat (100%). Backed by our 100% Guarantee.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunohistochemistry 1:500 - 1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000 - 1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
HeLa Nuclear Cell Lysate
Read Publications using
NB200-335 in the following applications:

  • B/N
    1 publication
  • 1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
0.09% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for NCOA6 Antibody

  • Activating signal cointegrator 2
  • AIB3NRC RAP250
  • Amplified in breast cancer protein 3
  • ASC-2
  • Cancer-amplified transcriptional coactivator ASC-2
  • KIAA0181PPAR-interacting protein
  • NRCamplified in breast cancer-3 protein
  • nuclear receptor coactivator 6
  • Nuclear receptor coactivator RAP250
  • Nuclear receptor-activating protein, 250 kDa
  • peroxisome proliferator-activated receptor interacting protein
  • PRIPPeroxisome proliferator-activated receptor-interacting protein
  • RAP250Thyroid hormone receptor-binding protein
  • thyroid hormone receptor binding protein
  • TRBPASC2activating signal cointegrator-2


Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Coactivates expression in an agonist- and AF2-dependent manner. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ERs), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Probably functions as a general coactivator, rather than just a nuclear receptor coactivator. May also be involved in the coactivation of the NF-kappa-B pathway. May coactivate expression via a remodeling of chromatin and its interaction with histone acetyltransferase proteins. Involved in placental, cardiac, hepatic and embryonic development. NCOA6 is over-expressed in several breast cancer cell lines.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NCOA6 Antibody (NB200-335)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: B/N, IF/IHC, WB.

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Showing Publications 1 - 8 of 8.
Publications using NB200-335 Applications Species
Oh GS, Kim SR, Lee ES et al. Regulation of Hepatic Gluconeogenesis by Nuclear Receptor Coactivator 6 Molecules and Cells 2022-04-30 [PMID: 35258009] (B/N, WB) B/N, WB
Kopanja D, Pandey A, Huang S et al. p19Arf Inhibits Aggressive Progression of H-ras driven Hepatocellular Carcinoma. Carcinogenesis 2017-12-08 [PMID: 29228217] (IF/IHC, Human) IF/IHC Human
Chen WD, Fu X, Dong B et al. Neonatal activation of the nuclear receptor CAR results in epigenetic memory and permanent change of drug metabolism in mouse liver. Hepatology 2012-10-01 [PMID: 22488010]
Vicent GP, Nacht AS, Font-Mateu J et al. Four enzymes cooperate to displace histone H1 during the first minute of hormonal gene activation. Genes Dev 2011-04-01 [PMID: 21447625]
Lee S, Kim DH, Goo YH et al. Crucial roles for interactions between MLL3/4 and INI1 in nuclear receptor transactivation. Mol Endocrinol 2009-05-01 [PMID: 19221051]
Garapaty S, Xu CF, Trojer P et al. Identification and characterization of a novel nuclear protein complex involved in nuclear hormone receptor-mediated gene regulation. J Biol Chem 2009-03-01 [PMID: 19131338]
Lee J, Saha PK, Yang QH et al. Targeted inactivation of MLL3 histone H3-Lys-4 methyltransferase activity in the mouse reveals vital roles for MLL3 in adipogenesis. Proc Natl Acad Sci U S A 2008-12-01 [PMID: 19047629]
Cho YW, Hong T, Hong S et al. PTIP associates with MLL3- and MLL4-containing histone H3 lysine 4 methyltransferase complex. J Biol Chem 2007-07-01 [PMID: 17500065]

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Research Areas for NCOA6 Antibody (NB200-335)

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Blogs on NCOA6.

ASC-2: An important co-activator of different nuclear receptors
ASC-2, also known as Nuclear Receptor Co-Activator 6, directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. It is involved in the co-activation of different nuclear receptors, such as for steroi...  Read full blog post.

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Gene Symbol NCOA6