Home » TRANCE/TNFSF11/RANK L » TRANCE/TNFSF11/RANK L Antibodies » TRANCE/TNFSF11/RANK L Antibody - BSA Free

TRANCE/TNFSF11/RANK L Antibody - BSA Free

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Western Blot: TRANCE/TNFSF11/RANK L Antibody - BSA Free [NB100-80849] - Rat liver tissue lysate with sRANK-L antibody at (A) 0.25 and (B) 0.5 ug/ml.
Western Blot: TRANCE/TNFSF11/RANK L Antibody - BSA Free [NB100-80849] - Rat liver tissue lysate with sRANK-L antibody at (A) 0.25 and (B) 0.5 ug/ml.
Immunohistochemistry-Paraffin: TRANCE/TNFSF11/RANK L Antibody - BSA Free [NB100-80849] - Staining of human small intestine tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Immunohistochemistry-Paraffin: TRANCE/TNFSF11/RANK L Antibody - BSA Free [NB100-80849] - Staining of human breast tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

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TRANCE/TNFSF11/RANK L Antibody - BSA Free Summary

Immunogen
A 14 aa synthetic peptide near the center of human TRANCE/TNFSF11/RANK L. The immunogen is located within aa 140 - 190 of TRANCE/TNFSF11/RANK L.
Epitope
Near the center, aa 140-190
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TNFSF11
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 0.25-0.5 ug/ml
Application Notes
Use in ICC/IF reported in scientific literature (PMID: 29524829).
Control
TRANCE/TNFSF11/RANK L Overexpression Lysate
Publications
Read Publications using
NB100-80849 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for TRANCE/TNFSF11/RANK L Antibody - BSA Free

  • CD254 antigen
  • CD254
  • ODF
  • OPGL
  • OPGLOPTB2
  • Osteoclast differentiation factor
  • Osteoprotegerin ligand
  • RANK L
  • RANKL
  • RANKLreceptor activator of nuclear factor kappa B ligand
  • Receptor activator of nuclear factor kappa-B ligand
  • sOdf
  • TNF-related activation-induced cytokine
  • TNFSF11
  • TRANCE
  • TRANCEODFhRANKL2
  • tumor necrosis factor (ligand) superfamily, member 11
  • tumor necrosis factor ligand superfamily member 11

Background

RANKL is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. There are three isoforms of RANKL. Human RANKL is a soluble 20 kDa polypeptide, comprising the TNF homologous region of RANKL (176 amino acid residues). This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor associated factor (TRAF) 6, which indicated that this protein may have a role in the regulation of cell apoptosis. RANKL deficient mice show severe osteoporesis and complete absence of osteoclasts as a result of lack of osteogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) - BSA Free [NB100-56508] - An intracellular stain was performed on MG-63 cells with RANK/TNFRSF11A Antibody (9A725) NB100-56508 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).
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TRANCE/TNFSF11/RANK L ...
NB100-80849
TRANCE/TNFSF11/RANK L Antibody - BSA Free
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P

Publications for TRANCE/TNFSF11/RANK L Antibody (NB100-80849)(5)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 2 applications: ICC/IF, IHC.


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Reviews for TRANCE/TNFSF11/RANK L Antibody (NB100-80849) (0)

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Product General Protocols

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WB Video Protocol
ICC/IF Video Protocol

FAQs for TRANCE/TNFSF11/RANK L Antibody (NB100-80849) (0)

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Control Lysate(s)

TRANCE/TNFSF11/RANK L Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for TRANCE/TNFSF11/RANK L Antibody (NB100-80849)

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Check out the latest blog posts on TRANCE/TNFSF11/RANK L.
The diverse functions of RANKL/TRANCE/TNFSF11
RANKL (also known as TNF-related activation-induced cytokine), or receptor activator of nuclear factor-?B ligand, was first discovered as a key player in the RANKL/RANK/OPG osteoclast formation pathway. Osteoclasts are large multinucleate cells t...  Read full blog post.
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Bioinformatics

Gene Symbol TNFSF11
Entrez
Uniprot