TRAF-2 Antibody (33A1293) - BSA Free

Western Blot: TRAF-2 Antibody (33A1293) [NB100-56715] - Analysis of TRAF2 in HeLa lysate using TRAF2 antibody at 2 ug/ml.

Immunohistochemistry-Paraffin: TRAF-2 Antibody (33A1293) [NB100-56715] - Human transitional cell carcinoma of the urinary bladder stained with TRAF2 antibody at 5 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 33A1293
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

TRAF-2 Antibody (33A1293) - BSA Free Summary

• Immunogen: Anti-TRAF2 monoclonal antibody was raised against a fusion protein corresponding to amino acids 205 to 222 of human TRAF2.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TRAF2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2:20-2:1000
  • Immunohistochemistry-Paraffin 1:10-1:500. Use reported in scientific literature (Galen et al (2008))
  • Western Blot 2 ug/ml
• Application Notes: In HeLa, an approx. 57 kDa band is observed.
• Control:

  HeLa Whole Cell Lysate
  TRAF-2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for TRAF-2 Antibody (33A1293) - BSA Free

• E3 ubiquitin-protein ligase TRAF2
• EC 6.3.2.-
• MGC:45012
• TNF receptor-associated factor 2
• TRAF2
• TRAF-2
• TRAP3
• TRAP3TRAP
• tumor necrosis factor type 2 receptor associated protein 3
• Tumor necrosis factor type 2 receptor-associated protein 3

Background

Tumor necrosis factor (TNF) induced signaling is mediated through association of TNF receptor (TNFR) with adaptor proteins, such as TNF receptor associated proteins (TRAFs). TRAFs form a family of cytoplasmic adapter proteins that mediate signal transduction from many members of the TNF-receptor superfamily (e.g. RANK, CD30, CD40, etc.) and the interleukin-1 receptor. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. Recent molecular cloning studies have lead to identification of six TRAFs (TRAF1-TRAF6). TRAF2 is a 502-amino acid protein. Mutagenic studies suggest that the N-terminal RING finger and two adjacent zinc fingers of TRAF2 are required for NF-kB activation, where as interaction with TNFR is mediated through C-terminus domain. Distinct domains in the N- and -C termini are also required for association with TRAF1 and protein kinase receptor interacting protein (RIP). TRAF2 is involved in cellular resistant to TNF-induced apoptosis. TRAF-2 deficient mice appeared normal at birth, however, they die prematurely, probably due to atrophy of thymus, spleen, muscle mass and lack of TRAF-2's cytoprotective role.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRAF-2 Antibody (NB100-56715)(5)

Publications using NB100-56715

• Wu G, Xu Y, Schultz RD et al. LILRB3 supports acute myeloid leukemia development and regulates T-cell antitumor immune responses through the TRAF2-cFLIP-NF-?B signaling axis Nature Cancer 2021-11-11 [PMID: 35122056]
• Sikorski K, Mehta A et al. A high-throughput pipeline for validation of antibodies. Nat Methods 2018-01-11 [PMID: 30377371] (Human)
  
  Details:
  Antibody validation based on denaturing gel electrophoresis of biotinylated cell lysates (PAGE) followed by mass spectrometry (MS) and antibody array analysis (MAP).
• Shanmugam R, Kusumanchi P, Cheng L et al. A water-soluble parthenolide analogue suppresses in vivo prostate cancer growth by targeting NFkappaB and generating reactive oxygen species. Prostate. 2010-07-01 [PMID: 20209491] (WB)
  
  Details:
  TRAF2 (IMG-162). WB: Androgen independent prostate cancer (CWR22Rv1) cell line, Fig 5.
• van Galen JC, Muris JJ, Giroth CP et al. Expression of TNF-receptor associated factor 2 correlates with poor progression-free survival time in ABC-like primary nodal diffuse large B-cell lymphomas. Histopathology. 2008-04-01 [PMID: 18312353] (IHC-P)
  
  Details:
  TRAF2 (IMG-162). IHC (paraffin): Human non-neoplastic lymphoid tissue from patients wtih activated B cell (ABC) like diffuse large B-cell lymphoma (DLBCL), Fig 1. Note: The TRF2 antibody was used at a 1:200 dilution, Fig 1.
• Hawari FI, Rouhani FN, Cui X et al. Release of full-length 55-kDa TNF receptor 1 in exosome-like vesicles: a mechanism for generation of soluble cytokine receptors. Proc Natl Acad Sci U S A. 2004-02-03 [PMID: 14745008]

Bioinformatics

• Gene Symbol: TRAF2
• Entrez:
  • 7186(Human)
• Uniprot:
  • Q12933()