TNF-alpha Antibody [Unconjugated]


Western blot shows conditioned media from CHO Chinese hamster ovary cell line either mock transfected or transfected with human TNF- alpha. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse TNF-a Antigen more
Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 4 hours. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse TNF-a Antigen more
TNF-a was detected in immersion fixed RAW 264.7 mouse monocyte/ macrophage cell line treated with LPS using Human/Mouse TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA) at 10 µg/mL for 3 hours more
TNF‑ alpha was detected in immersion fixed activated mouse T Cells using 15 µg/mL Human/Mouse TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑410‑NA) for 3 hours at room temperature. more
TNF‑ alpha was detected in immersion fixed paraffin-embedded sections of mouse spleen tissue using Goat Anti-Human/Mouse TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA) at more
Recombinant Mouse TNF-a (410-MT) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Mouse TNF-a (0.1 ng/mL) is neutralized more
TNF‑ alpha was detected in immersion fixed paraffin-embedded sections of Human Spleen using Goat Anti-Human/Mouse TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA) at more

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, B/N, CyTOF-ready, ELISA(Cap), Flow, ICC/IF

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TNF-alpha Antibody [Unconjugated] Summary

E. coli-derived recombinant mouse TNF-alpha
Accession # P06804
Detects mouse TNF-alpha  in ELISAs. Detects human and mouse TNF-alpha in Western blots. In sandwich immunoassays, approximately 50% cross-reactivity with recombinant rat TNF‑ alpha is observed.
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 3-15 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Neutralization 0.1-0.4 ug/mL
  • Western Blot 2 ug/mL
Application Notes
ELISA Capture:Mouse TNF-alpha Antibody (Catalog # AF-410-NA)
ELISA Detection: Mouse TNF-alpha Biotinylated Antibody (Catalog # BAF410)
Standard: Recombinant Mouse TNF-alpha aa 80-235 (Catalog # 410-MT)
Read Publications using
AF-410-NA in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TNF-alpha Antibody [Unconjugated]

  • APC1 protein
  • Cachectin
  • Cachetin
  • DIF
  • TNF
  • TNF, monocyte-derived
  • TNFA
  • TNF-A
  • TNFalpha
  • TNF-alpha
  • TNF-alphacachectin
  • TNFATNF, macrophage-derived
  • TNFG1F
  • TNFSF2
  • TNFSF2TNF superfamily, member 2
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • Tumor necrosis factor ligand superfamily member 2
  • tumor necrosis factor
  • tumor necrosis factor-alpha


Tumor necrosis factor alpha (TNF-alpha, TNF- alpha , TNFA ), also known as Cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism. TNF-alpha is produced by several lymphoid cells as well as by astrocytes, endothelial cells, and smooth muscle cells. Mouse TNF-alpha consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 179 aa extracellular domain (ECD). Within the ECD, mouse TNF-alpha shares 94% aa sequence identity with rat and 70%-77% with bovine, canine, cotton rat, equine, feline, human, porcine, and rhesus TNF-alpha. TNF-alpha is produced by a wide variety of immune, epithelial, endothelial, and tumor cells. TNF-alpha is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface. Cell surface TNF-alpha can induce the lysis of neighboring tumor cells and virus infected cells, and it can generate its own downstream cell signaling following ligation by soluble TNFR I. Shedding of membrane bound TNF-alpha by TACE/ADAM17 releases the bioactive cytokine, a 55 kDa molecular weight soluble trimer of the TNF-alpha extracellular domain. TNF-alpha binds the ubiquitous 55-60 kDa TNF RI and the hematopoietic cell-restricted 80 kDa TNF RII, both of which are also expressed as homotrimers present on virtually all cell types. Both type I and type II receptors bind TNF-alpha with comparable affinity, although only TNF RI contains a cytoplasmic death domain which triggers the activation of apoptosis. Soluble forms of both types of receptors are released and can neutralize the biological activity of TNF-alpha.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TNF-alpha Antibody (AF-410-NA)(81)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Porcine.

We have publications tested in 15 applications: Bioassay, Cell Culture, ELISA Development, ELISA Development (Capture), Flow Cytometry, Functional Assay, ICC, IHC, IHC-Fr, IHC-P, Immunoprecipitation, In Vivo, Luminex Development, Neutralization, Western Blot.

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ELISA Development (Capture)
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Showing Publications 1 - 10 of 81. Show All 81 Publications.
Publications using AF-410-NA Applications Species
Oppezzo, A;Monney, L;Kilian, H;Slimani, L;Maczkowiak-Chartois, F;Rosselli, F; Fanca deficiency is associated with alterations in osteoclastogenesis that are rescued by TNF? Cell & bioscience 2023-06-24 [PMID: 37355617] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Y Hwang, HC Kim, EJ Shin Effect of rottlerin on astrocyte phenotype polarization after trimethyltin insult in the dentate gyrus of mice Journal of Neuroinflammation, 2022-06-11;19(1):142. 2022-06-11 [PMID: 35690821] (Western Blot, Mouse) Western Blot Mouse
B Bai, H Li, L Han, Y Mei, C Hu, Q Mei, J Xu, X Liu Molecular mechanism of the TGF?beta/Smad7 signaling pathway in ulcerative colitis Molecular Medicine Reports, 2022-02-09;25(4):. 2022-02-09 [PMID: 35137923] (In Vivo, Mouse) In Vivo Mouse
G Theocharid, BE Thomas, D Sarkar, HL Mumme, WJR Pilcher, B Dwivedi, T Sandoval-S, RF Sîrbulescu, A Kafanas, I Mezghani, P Wang, A Lobao, IS Vlachos, B Dash, HC Hsia, V Horsley, SS Bhasin, A Veves, M Bhasin Single cell transcriptomic landscape of diabetic foot ulcers Nature Communications, 2022-01-10;13(1):181. 2022-01-10 [PMID: 35013299] (IHC, Human) IHC Human
Y Luo, F Yao, X Hu, Y Li, Y Chen, Z Li, Z Zhu, S Yu, D Tian, L Cheng, M Zheng, J Jing M1 macrophages impair tight junctions between endothelial cells after spinal cord injury Brain research bulletin, 2022-01-04;0(0):. 2022-01-04 [PMID: 34995751] (Neutralization, Mouse) Neutralization Mouse
MS AlQranei, LT Senbanjo, H Aljohani, T Hamza, MA Chellaiah Lipopolysaccharide- TLR-4 Axis regulates Osteoclastogenesis independent of RANKL/RANK signaling BMC immunology, 2021-03-25;22(1):23. 2021-03-25 [PMID: 33765924] (Neutralization, Mouse) Neutralization Mouse
RS Najjar, NS Akhavan, S Pourafshar, BH Arjmandi, RG Feresin Cornus officinalis var. koreana Kitam polyphenol extract decreases pro-inflammatory markers in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages by reducing Akt phosphorylation Journal of ethnopharmacology, 2020-12-24;0(0):113734. 2020-12-24 [PMID: 33359857] (Western Blot, Mouse) Western Blot Mouse
T Yoshida, NA Das, AJ Carpenter, R Izadpanah, SA Kumar, S Gautam, SB Bender, U Siebenlist, B Chandrasek Minocycline reverses IL-17A/TRAF3IP2-mediated p38 MAPK/NF-kappaB/iNOS/NO-dependent cardiomyocyte contractile depression and death Cell. Signal., 2020-06-15;73(0):109690. 2020-06-15 [PMID: 32553549] (Neutralization, Western Blot, Mouse) Neutralization, Western Blot Mouse
Z Li, C Zhang, JX Du, J Zhao, MT Shi, MW Jin, H Liu Adipocytes promote tumor progression and induce PD-L1 expression via TNF-&amp;alpha/IL-6 signaling Cancer Cell Int., 2020-05-20;20(0):179. 2020-05-20 [PMID: 32477009] (Neutralization, Human, Mouse) Neutralization Human, Mouse
Y Takagaki, SM Lee, Z Dongqing, M Kitada, K Kanasaki, D Koya Endothelial autophagy deficiency induces IL6 - dependent endothelial mesenchymal transition and organ fibrosis Autophagy, 2020-01-22;0(0):1-10. 2020-01-22 [PMID: 31965901] (Neutralization, Human) Neutralization Human
Show All 81 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

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FAQs for TNF-alpha Antibody (AF-410-NA). (Showing 1 - 4 of 4 FAQs).

  1. I need to know about TNF-alpha antibody kits for mice.
    • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
  2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
    • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
  3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
    • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
  4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
    • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

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Blogs on TNF-alpha. Showing 1-10 of 13 blog posts - Show all blog posts.

Unlocking the Potential of Biosimilars in Immuno-Oncology
By Jennifer Jones, M.S.Biosimilar Antibodies: Imitation Meets InnovationIn the ever-evolving medical landscape, a new class of pharmaceuticals is emerging as a game-changer, poised to transform the way we approach...  Read full blog post.

Tired T cells: Hypoxia Drives T cell Exhaustion in the Tumor Microenvironment
By Hunter MartinezThe paradigm shifting view of the immune system being leveraged to target cancer has led to numerous therapeutic breakthroughs. One major cell group responsible for this revelation is a T cell. ...  Read full blog post.

Immune Cell Metabolic Flux Influences Type I Diabetes
By Hunter MartinezWhat is Immunometabolism?It is well established that abnormal metabolic environments can be a risk factor for disease development. One characteristic example is the role of dyslipidemia (high lev...  Read full blog post.

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Liver ASK1 activates autophagy to protect against hepatic fat accumulation, non-alcoholic steatohepatitis and fibrosis
By Jamshed Arslan, Pharm. D., PhD. The most common chronic liver disorder worldwide is non-alcoholic fatty liver disease (NAFLD). This obesity-linked disorder can manifest as hepatic fat accumulation (steatosis) wit...  Read full blog post.

Metabolic Syndrome: Symptoms and associated disease states
By Jamshed Arslan, Pharm. D., PhD. What is metabolic syndrome?It was 1988 when, after decades of research, Dr. GM Reaven    of the Stanford University, CA, explained the relationship between four diseases:...  Read full blog post.

Increased wild type FUS levels in ALS patients lead to a toxic microenvironment and motor neuron neurodegeneration
By Michalina Hanzel, PhDFUS mutations in Amyotrophic Lateral SclerosisFused in sarcoma (FUS) is a ribonucleoprotein that continuously shuttles between the nucleus and the cytoplasm to regulate pre-mRNA splicing, mRN...  Read full blog post.

You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers
By Jamshed Arslan Pharm.D. Natural killer (NK) cells are lymphocytes of the innate immune system that were first discovered for their “natural” ability to kill cancer cells. To use NK cells as anti-cancer therapy, t...  Read full blog post.

Showing 1-10 of 13 blog posts - Show all blog posts.
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Gene Symbol TNF