Western blot shows conditioned media from CHO Chinese hamster ovary cell line either mock transfected or transfected with human TNF- alpha. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse TNF-a Antigen ...read more
Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 4 hours. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse TNF-a Antigen ...read more
TNF-a was detected in immersion fixed RAW 264.7 mouse monocyte/ macrophage cell line treated with LPS using Human/Mouse TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA) at 10 µg/mL for 3 hours ...read more
TNF‑ alpha was detected in immersion fixed activated mouse T Cells using 15 µg/mL Human/Mouse TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑410‑NA) for 3 hours at room temperature. ...read more
TNF‑ alpha was detected in immersion fixed paraffin-embedded sections of mouse spleen tissue using Goat Anti-Human/Mouse TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA) at ...read more
Recombinant Mouse TNF-a (410-MT) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Mouse TNF-a (0.1 ng/mL) is neutralized ...read more
TNF‑ alpha was detected in immersion fixed paraffin-embedded sections of Human Spleen using Goat Anti-Human/Mouse TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA) at ...read more
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for TNF-alpha Antibody [Unconjugated]
TNFSF2TNF superfamily, member 2
tumor necrosis factor (TNF superfamily, member 2)
tumor necrosis factor alpha
Tumor necrosis factor ligand superfamily member 2
tumor necrosis factor
tumor necrosis factor-alpha
Tumor necrosis factor alpha (TNF-alpha, TNF- alpha , TNFA ), also
known as Cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily.
It is a pleiotropic molecule that plays a central role in inflammation, immune
system development, apoptosis, and lipid metabolism. TNF-alpha is produced by
several lymphoid cells as well as by astrocytes, endothelial cells, and smooth
muscle cells. Mouse TNF-alpha consists of a 35 amino acid (aa) cytoplasmic
domain, a 21 aa transmembrane segment, and a 179 aa extracellular domain (ECD).
Within the ECD, mouse TNF-alpha shares 94% aa sequence identity with rat and 70%-77%
with bovine, canine, cotton rat, equine, feline, human, porcine, and rhesus
TNF-alpha. TNF-alpha is produced by a wide variety of immune, epithelial,
endothelial, and tumor cells. TNF-alpha is assembled intracellularly to form a
noncovalently linked homotrimer which is expressed on the cell surface. Cell
surface TNF-alpha can induce the lysis of neighboring tumor cells and virus
infected cells, and it can generate its own downstream cell signaling following
ligation by soluble TNFR I. Shedding of membrane bound TNF-alpha by TACE/ADAM17
releases the bioactive cytokine, a 55 kDa molecular weight soluble trimer of
the TNF-alpha extracellular domain. TNF-alpha binds the ubiquitous 55-60 kDa
TNF RI and the hematopoietic cell-restricted 80 kDa TNF RII, both of which are
also expressed as homotrimers present on virtually all cell types. Both type I
and type II receptors bind TNF-alpha with comparable affinity, although only
TNF RI contains a cytoplasmic death domain which triggers the activation of
apoptosis. Soluble forms of both types of receptors are released and can
neutralize the biological activity of TNF-alpha.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for TNF-alpha Antibody (AF-410-NA). (Showing 1 - 4 of 4 FAQs).
I need to know about TNF-alpha antibody kits for mice.
We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.
CD86 - I work in tandem with CD80 CD86 belongs to the immunoglobulin superfamily of proteins that drive innate and adaptive immune responses. It is an 80kD co-stimulatory molecule for the priming and activation of naive and memory T-cells, respectively. CD86 is expressed on activated ... Read full blog post.
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