TNF-alpha Antibody [Unconjugated]

Western blot shows conditioned media from CHO Chinese hamster ovary cell line either mock transfected or transfected with human TNF- alpha. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for TNF‑ alpha at approximately 14 and 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 μg/mL LPS for 4 hours. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for TNF‑ alpha at approximately 14 and 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

TNF‑ alpha was detected in immersion fixed activated mouse T Cells using 15 µg/mL Human/Mouse TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑410‑NA) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Recombinant Mouse TNF‑ alpha (Catalog # 410-MT) induces cytotoxicity in the the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Mouse TNF‑ alpha (0.1 ng/mL) is neutralized (green line) by increasing concentrations of Human/Mouse TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA). The ND50 is typically 1.5-10 ng/mL in the presence of the metabolic inhibitor actinomycin D.

TNF‑ alpha was detected in immersion fixed RAW 264.7 mouse monocyte/ macrophage cell line treated with LPS using Human/Mouse TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu, Mu
• Applications: WB, B/N, CyTOF-ready, ELISA(Cap), Flow, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

TNF-alpha Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse TNF-alpha (R&D Systems, Catalog # 410-MT)
  Leu80-Leu235
  Accession # P06804
• Specificity: Detects mouse TNF-alpha in ELISAs. Detects human and mouse TNF-alpha in Western blots. In sandwich immunoassays, approximately 50% cross-reactivity with recombinant rat TNF‑ alpha is observed.
• Details of Functionality: ActivityAssaywCitation not found None None None None None
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TNF
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Immunocytochemistry 5-15 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Neutralization 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Capture:Mouse TNF-alpha Antibody (Catalog # AF-410-NA)
  ELISA Detection: Mouse TNF-alpha Biotinylated Antibody (Catalog # BAF410)
  Standard: Recombinant Mouse TNF-alpha aa 80-235 (Catalog # 410-MT)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TNF-alpha Antibody [Unconjugated]

• APC1 protein
• Cachectin
• Cachetin
• DIF
• TNF
• TNF, monocyte-derived
• TNFA
• TNF-A
• TNFalpha
• TNF-alpha
• TNF-alphacachectin
• TNFATNF, macrophage-derived
• TNFG1F
• TNFSF1A
• TNFSF2
• TNFSF2TNF superfamily, member 2
• tumor necrosis factor (TNF superfamily, member 2)
• tumor necrosis factor alpha
• Tumor necrosis factor ligand superfamily member 2
• tumor necrosis factor
• tumor necrosis factor-alpha

Background

Tumor necrosis factor alpha (TNF-alpha, TNF- alpha, TNFA ), also known as Cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism. TNF-alpha is produced by several lymphoid cells as well as by astrocytes, endothelial cells, and smooth muscle cells. Mouse TNF-alpha consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 179 aa extracellular domain (ECD). Within the ECD, mouse TNF-alpha shares 94% aa sequence identity with rat and 70%-77% with bovine, canine, cotton rat, equine, feline, human, porcine, and rhesus TNF-alpha. TNF-alpha is produced by a wide variety of immune, epithelial, endothelial, and tumor cells. TNF-alpha is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface. Cell surface TNF-alpha can induce the lysis of neighboring tumor cells and virus infected cells, and it can generate its own downstream cell signaling following ligation by soluble TNFR I. Shedding of membrane bound TNF-alpha by TACE/ADAM17 releases the bioactive cytokine, a 55 kDa molecular weight soluble trimer of the TNF-alpha extracellular domain. TNF-alpha binds the ubiquitous 55-60 kDa TNF RI and the hematopoietic cell-restricted 80 kDa TNF RII, both of which are also expressed as homotrimers present on virtually all cell types. Both type I and type II receptors bind TNF-alpha with comparable affinity, although only TNF RI contains a cytoplasmic death domain which triggers the activation of apoptosis. Soluble forms of both types of receptors are released and can neutralize the biological activity of TNF-alpha.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TNF-alpha Antibody (AF-410-NA)(69)

Publications using AF-410-NA

• Q Zhou, X Wu, X Wang, Z Yu, T Pan, Z Li, X Chang, Z Jin, J Li, Z Zhu, B Liu, L Su The reciprocal interaction between tumor cells and activated fibroblasts mediated by TNF-alpha/IL-33/ST2L signaling promotes gastric cancer metastasis Oncogene, 2019;0(0):. 2019 [PMID: 31659258] (Cell Culture, Human)
• Y Xu, Y Xu, Y Zhu, H Sun, C Juguilon, F Li, D Fan, L Yin, Y Zhang Macrophage miR-34a Is a Key Regulator of Cholesterol Efflux and Atherosclerosis Mol. Ther., 2019;0(0):. 2019 [PMID: 31604677] (Western Blot, Human)
• P Wang, X Qi, G Xu, J Liu, J Guo, X Li, X Ma, H Sun CCL28 promotes locomotor recovery after spinal cord injury via recruiting regulatory T cells Aging (Albany NY), 2019;11(18):7402-7415. 2019 [PMID: 31557129] (Neutralization, Mouse)
• X Zhang, D He, S Gao, Y Wei, L Wang Aspergillus�fumigatus enhances human NK cell activity by regulating M1 macrophage polarization Mol Med Rep, 2019;20(2):1241-1249. 2019 [PMID: 31173233] (Cell Culture, Human)
• OJ Sul, M Rajasekara, HJ Park, JH Suh, HS Choi MicroRNA-29b Enhances Osteoclast Survival by Targeting BCL-2-Modifying Factor after Lipopolysaccharide Stimulation Oxid Med Cell Longev, 2019;2019(0):6018180. 2019 [PMID: 31093317] (Western Blot, Mouse)
• J Pido-Lopez, B Tanudjojo, S Farag, MK Bondulich, R Andre, SJ Tabrizi, GP Bates Inhibition of tumour necrosis factor alpha in the R6/2 mouse model of Huntington&#039;s disease by etanercept treatment Sci Rep, 2019;9(1):7202. 2019 [PMID: 31076648] (Western Blot, Mouse)
• JA Wixey, KM Lee, SM Miller, K Goasdoue, PB Colditz, S Tracey Bjo, KK Chand Neuropathology in intrauterine growth restricted newborn piglets is associated with glial activation and proinflammatory status in the brain J Neuroinflammation, 2019;16(1):5. 2019 [PMID: 30621715] (IHC-P, Porcine)
• C Widmann, C Gandin, A Petit-Pait, M Lazdunski, C Heurteaux The Traditional Chinese Medicine MLC901 inhibits inflammation processes after focal cerebral ischemia Sci Rep, 2018;8(1):18062. 2018 [PMID: 30584250] (IHC, Mouse)
• RF Righetti, TM Dos Santos, LDN Camargo, LRCRB Aristótele, S Fukuzaki, FCR de Souza, FPR Santana, MVR de Agrela, MM Cruz, MIC Alonso-Val, IS Genaro, BM Saraiva-Ro, EA Leick, MA Martins, CM Prado, IFLC Tibério Protective Effects of Anti-IL17 on Acute Lung Injury Induced by LPS in Mice Front Pharmacol, 2018;9(0):1021. 2018 [PMID: 30337870] (IHC, Mouse)
• M Kremer, I Yalcin, Y Goumon, X Wurtz, L Nexon, D Daniel, S Megat, RA Ceredig, C Ernst, G Turecki, V Chavant, JF Theroux, A Lacaud, LE Joganah, V Lelievre, D Massotte, PE Lutz, R Gilsbach, E Salvat, M Barrot A dual noradrenergic mechanism for the relief of neuropathic allodynia by the antidepressant drugs duloxetine and amitriptyline J. Neurosci., 2018;0(0):. 2018 [PMID: 30249798] (Western Blot, Mouse)
• CJ Lee, SJ Moon, JH Jeong, S Lee, MH Lee, SM Yoo, HS Lee, HC Kang, JY Lee, WS Lee, HJ Lee, EK Kim, JY Jhun, ML Cho, JK Min, YY Cho Kaempferol targeting on the fibroblast growth factor receptor 3-ribosomal S6 kinase 2 signaling axis prevents the development of rheumatoid arthritis Cell Death Dis, 2018;9(3):401. 2018 [PMID: 29540697] (ELISA Development (Capture), Mouse)
• K Zenke, M Muroi, KI Tanamoto AKT1 distinctively suppresses MyD88-depenedent and TRIF-dependent Toll-like receptor signaling in a kinase activity-independent manner Cell. Signal., 2017;43(0):32-39. 2017 [PMID: 29242168] (Western Blot, Mouse)
• T Kottke, L Evgin, KG Shim, D Rommelfang, N Boisgeraul, S Zaidi, RM Diaz, J Thompson, E Ilett, M Coffey, P Selby, H Pandha, K Harrington, A Melcher, R Vile Subversion of NK-cell and TNF? Immune Surveillance Drives Tumor Recurrence Cancer Immunol Res, 2017;5(11):1029-1045. 2017 [PMID: 29038298] (Neutralization, Mouse)
• SH Lee, JY Kwon, SY Kim, K Jung, ML Cho Interferon-gamma regulates inflammatory cell death by targeting necroptosis in experimental autoimmune arthritis Sci Rep, 2017;7(1):10133. 2017 [PMID: 28860618] (ELISA Development (Capture), Mouse)
• Y Oh, YS Kwon, BD Jung Anti-inflammatory Effects of the Natural Compounds Cortex Phellodendri and Humulus japonicus on Pelvic Inflammatory Disease in Mice Int J Med Sci, 2017;14(8):729-734. 2017 [PMID: 28824307] (ELISA Development (Capture), Mouse)
• N Matsushita, MT Hassanein, M Martinez-C, R Lazaro, SW French, W Xie, K Lai, M Karin, H Tsukamoto Gender difference in NASH susceptibility: Roles of hepatocyte Ikk? and Sult1e1 PLoS ONE, 2017;12(8):e0181052. 2017 [PMID: 28797077] (IHC-P, Mouse)
• KJ Chung, A Chatzigeor, M Economopou, R Garcia-Mar, VI Alexaki, I Mitroulis, M Nati, J Gebler, T Ziemssen, SE Goelz, J Phieler, JH Lim, KP Karalis, T Papayannop, M Blüher, G Hajishenga, T Chavakis A self-sustained loop of inflammation-driven inhibition of beige adipogenesis in obesity Nat. Immunol., 2017;0(0):. 2017 [PMID: 28414311] (Neutralization, Mouse)
• T Tsunekawa, R Banno, A Mizoguchi, M Sugiyama, T Tominaga, T Onoue, D Hagiwara, Y Ito, S Iwama, M Goto, H Suga, Y Sugimura, H Arima Deficiency of PTP1B Attenuates Hypothalamic Inflammation via Activation of the JAK2-STAT3 Pathway in Microglia EBioMedicine, 2017;0(0):. 2017 [PMID: 28094236] (IHC-Fr, Mouse)
• C Dempsey Inhibiting the NLRP3 inflammasome with MCC950 promotes non-phlogistic clearance of amyloid-? and cognitive function in APP/PS1 mice Brain Behav. Immun, 2016;0(0):. 2016 [PMID: 28003153] (ELISA Development (Capture), Mouse)
• M C Muñoz Chronic blockade of the AT2 receptor with PD123319 impairs insulin signaling in C57BL/6 mice Peptides, 2016;0(0):. 2016 [PMID: 27979738] (Western Blot, Mouse)
• Yoonsook Kim Oryza sativa (Rice) Hull Extract Inhibits Lipopolysaccharide-Induced Inflammatory Response in RAW264.7 Macrophages by Suppressing Extracellular Signal-regulated Kinase, c-Jun N-terminal Kinase, and Nuclear Factor-?B Activation Pharmacogn Mag, 2016;12(48):295-301. 2016 [PMID: 27867272] (ELISA Development (Capture), Mouse)
• SY Lim, AE Yuzhalin, AN Gordon-Wee, RJ Muschel Tumor-infiltrating monocytes/macrophages promote tumor invasion and migration by upregulating S100A8 and S100A9 expression in cancer cells Oncogene, 2016;0(0):. 2016 [PMID: 27086923] (Neutralization, Mouse)
• Clatworthy M, Harford S, Mathews R, Smith K FcgammaRIIb inhibits immune complex-induced VEGF-A production and intranodal lymphangiogenesis. Proc Natl Acad Sci U S A, 2014;111(50):17971-6. 2014 [PMID: 25475856] (Neutralization, Mouse)
• Lambertsen K, Ostergaard K, Clausen B, Hansen S, Stenvang J, Thorsen S, Meldgaard M, Kristensen B, Hansen P, Sorensen G, Finsen B No effect of ablation of surfactant protein-D on acute cerebral infarction in mice. J Neuroinflammation, 2014;11(0):123. 2014 [PMID: 25038795] (Functional Assay, Mouse)
• Ilett E, Kottke T, Donnelly O, Thompson J, Willmon C, Diaz R, Zaidi S, Coffey M, Selby P, Harrington K, Pandha H, Melcher A, Vile R Cytokine conditioning enhances systemic delivery and therapy of an oncolytic virus. Mol Ther, 2014;22(10):1851-63. 2014 [PMID: 24957982] (Neutralization, Mouse)
• Ingham V, Williams A, Bate C Glimepiride reduces CD14 expression and cytokine secretion from macrophages. J Neuroinflammation, 2014;11(0):115. 2014 [PMID: 24952384] (ELISA Development (Capture), Mouse)
• Shikata F, Sakaue T, Nakashiro K, Okazaki M, Kurata M, Okamura T, Okura M, Ryugo M, Nakamura Y, Yasugi T, Higashiyama S, Izutani H Pathophysiology of lung injury induced by common bile duct ligation in mice. PLoS ONE, 2014;9(4):e94550. 2014 [PMID: 24733017] (IHC-P, Mouse)
• Ramkhelawon B, Hennessy E, Menager M, Ray T, Sheedy F, Hutchison S, Wanschel A, Oldebeken S, Geoffrion M, Spiro W, Miller G, McPherson R, Rayner K, Moore K Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity. Nat Med, 2014;20(4):377-84. 2014 [PMID: 24584118] (Bioassay, Mouse)
• Hillian, Antoinet, McMullen, Megan R, Sebastian, Becky M, Roychowdhury, Sanjoy, Kashyap, Sangeeta, Schauer, Philip R, Kirwan, John P, Feldstein, Ariel E, Nagy, Laura E Mice lacking C1q are protected from high fat diet-induced hepatic insulin resistance and impaired glucose homeostasis. J Biol Chem, 2013;288(31):22565-75. 2013 [PMID: 23788643] (IHC-Fr, Mouse)
• Junkins R, MacNeil A, Wu Z, McCormick C, Lin T Regulator of calcineurin 1 suppresses inflammation during respiratory tract infections. J Immunol, 2013;190(10):5178-86. 2013 [PMID: 23589609] (ELISA Development (Capture), Mouse)
• Li B, Shi Y, Shu J, Gao J, Wu P, Tang S Wingless-type mammary tumor virus integration site family, member 5A (Wnt5a) regulates human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein 120 (gp120)-induced expression of pro-inflammatory cytokines via the Ca2+/calmodulin-dependent protein kinase II (CaMKII) and c-Jun N-terminal kinase (JNK) signaling pathways. J Biol Chem, 2013;288(19):13610-9. 2013 [PMID: 23539626] (Western Blot, Mouse)
• Kim A, Feng P, Ohkuri T, Sauers D, Cohn ZJ, Chai J, Nelson T, Bachmanov AA, Huang L, Wang H Defects in the peripheral taste structure and function in the MRL/lpr mouse model of autoimmune disease. PLoS ONE, 2012;7(4):e35588. 2012 [PMID: 22536412] (IHC-Fr, Mouse)
• Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L Response patterns of cytokines/chemokines in two murine strains after irradiation. Cytokine, 2012;58(2):169-77. 2012 [PMID: 22277799] (Luminex Development, Mouse)
• Fisher DT, Chen Q, Skitzki JJ, Muhitch JB, Zhou L, Appenheimer MM, Vardam TD, Weis EL, Passanese J, Wang WC, Gollnick SO, Dewhirst MW, Rose-John S, Repasky EA, Baumann H, Evans SS IL-6 trans-signaling licenses mouse and human tumor microvascular gateways for trafficking of cytotoxic T cells. J. Clin. Invest., 2011;121(10):3846-59. 2011 [PMID: 21926464] (Neutralization, Mouse)
• Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin. J. Immunol., 2009;183(9):5799-806. 2009 [PMID: 19812208] (ELISA Development, Mouse)
• Koronyo-Hamaoui M, Ko MK, Koronyo Y, Azoulay D, Seksenyan A, Kunis G, Pham M, Bakhsheshian J, Rogeri P, Black KL, Farkas DL, Schwartz M Attenuation of AD-like neuropathology by harnessing peripheral immune cells: local elevation of IL-10 and MMP-9. J. Neurochem., 2009;111(6):1409-24. 2009 [PMID: 19780903] (IHC-Fr, Mouse)
• Baluk P, Yao LC, Feng J, Romano T, Jung SS, Schreiter JL, Yan L, Shealy DJ, McDonald DM TNF-alpha drives remodeling of blood vessels and lymphatics in sustained airway inflammation in mice. J. Clin. Invest., 2009;119(10):2954-64. 2009 [PMID: 19759514] (IHC-Fr, Mouse)
• Wendt W, Schulten R, Stichel CC, Lubbert H Intra- versus extracellular effects of microglia-derived cysteine proteases in a conditioned medium transfer model. J. Neurochem., 2009;110(6):1931-41. 2009 [PMID: 19627446] (Western Blot, Mouse)
• Nold-Petry CA, Nold MF, Nielsen JW, Bustamante A, Zepp JA, Storm KA, Hong JW, Kim SH, Dinarello CA Increased cytokine production in IL-18 receptor alpha-deficient cells is associated with dysregulation of suppressors of cytokine signaling (SOCS). J. Biol. Chem., 2009;0(0):. 2009 [PMID: 19592492] (ELISA Development, Mouse)
• Joly S, Francke M, Ulbricht E, Beck S, Seeliger M, Hirrlinger P, Hirrlinger J, Lang KS, Zinkernagel M, Odermatt B, Samardzija M, Reichenbach A, Grimm C, Reme CE Cooperative phagocytes: resident microglia and bone marrow immigrants remove dead photoreceptors in retinal lesions. Am. J. Pathol., 2009;174(6):2310-23. 2009 [PMID: 19435787] (IHC, Mouse)
• Wieland CW, van der Windt GJ, Florquin S, McKenzie AN, van der Poll T ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis. Microbes Infect., 2009;11(4):524-30. 2009 [PMID: 19286469] (ELISA Development, Mouse)
• Audoy-Remus J, Richard JF, Soulet D, Zhou H, Kubes P, Vallieres L Rod-Shaped monocytes patrol the brain vasculature and give rise to perivascular macrophages under the influence of proinflammatory cytokines and angiopoietin-2. J. Neurosci., 2008;28(41):10187-99. 2008 [PMID: 18842879] (Neutralization, Mouse)
• Lee SJ, Nam WD, Na HJ, Cho YL, Ha KS, Hwang JY, Lee H, Kim SO, Kwon YG, Kim YM CT20126, a novel immunosuppressant, prevents collagen-induced arthritis through the down-regulation of inflammatory gene expression by inhibiting NF-kappaB activation. Biochem. Pharmacol., 2008;76(1):79-90. 2008 [PMID: 18513703] (IHC-P, Mouse)
• Lahat N, Rahat MA, Kinarty A, Weiss-Cerem L, Pinchevski S, Bitterman H Hypoxia enhances lysosomal TNF-alpha degradation in mouse peritoneal macrophages. Am. J. Physiol., Cell Physiol., 2008;295(1):C2-12. 2008 [PMID: 18434619] (Flow Cytometry, Mouse)
• Wieland CW, van der Windt GJ, Wiersinga WJ, Florquin S, van der Poll T CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis. Immunology, 2008;125(2):272-9. 2008 [PMID: 18393969] (ELISA Development, Mouse)
• Muranski P, Boni A, Antony PA, Cassard L, Irvine KR, Kaiser A, Paulos CM, Palmer DC, Touloukian CE, Ptak K, Gattinoni L, Wrzesinski C, Hinrichs CS, Kerstann KW, Feigenbaum L, Chan CC, Restifo NP Tumor-specific Th17-polarized cells eradicate large established melanoma. Blood, 2008;112(2):362-73. 2008 [PMID: 18354038] (ELISA Development, Mouse)
• Kim HG, Kim NR, Gim MG, Lee JM, Lee SY, Ko MY, Kim JY, Han SH, Chung DK Lipoteichoic acid isolated from Lactobacillus plantarum inhibits lipopolysaccharide-induced TNF-alpha production in THP-1 cells and endotoxin shock in mice. J. Immunol., 2008;180(4):2553-61. 2008 [PMID: 18250466] (ELISA Development, Mouse)
• Cunha TM, Verri WA, Jr, Fukada SY, Guerrero AT, Santodomingo-Garzon T, Poole S, Parada CA, Ferreira SH, Cunha FQ TNF-alpha and IL-1beta mediate inflammatory hypernociception in mice triggered by B1 but not B2 kinin receptor. Eur. J. Pharmacol., 2007;573(1):221-9. 2007 [PMID: 17669394] (ELISA Development, Mouse)
• Amigo M, Paya M, De Rosa S, Terencio MC Antipsoriatic effects of avarol-3'-thiosalicylate are mediated by inhibition of TNF-alpha generation and NF-kappaB activation in mouse skin. Br. J. Pharmacol., 2007;152(3):353-65. 2007 [PMID: 17641670] (IHC-P, Mouse)
• Watanabe H, Kubo M, Numata K, Takagi K, Mizuta H, Okada S, Ito T, Matsukawa A Overexpression of suppressor of cytokine signaling-5 in T cells augments innate immunity during septic peritonitis. J. Immunol., 2006;177(12):8650-7. 2006 [PMID: 17142765] (ELISA Development, Mouse)
• Penido C, Conte FP, Chagas MS, Rodrigues CA, Pereira JF, Henriques MG Antiinflammatory effects of natural tetranortriterpenoids isolated from Carapa guianensis Aublet on zymosan-induced arthritis in mice. Inflamm. Res., 2006;55(11):457-64. 2006 [PMID: 17122962] (ELISA Development, Mouse)
• Hajjar AM, Harvey MD, Shaffer SA, Goodlett DR, Sjostedt A, Edebro H, Forsman M, Bystrom M, Pelletier M, Wilson CB, Miller SI, Skerrett SJ, Ernst RK Lack of in vitro and in vivo recognition of Francisella tularensis subspecies lipopolysaccharide by Toll-like receptors. Infect. Immun., 2006;74(12):6730-8. 2006 [PMID: 16982824] (ELISA Development, Mouse)
• Butovsky O, Koronyo-Hamaoui M, Kunis G, Ophir E, Landa G, Cohen H, Schwartz M Glatiramer acetate fights against Alzheimer&apos;s disease by inducing dendritic-like microglia expressing insulin-like growth factor 1. Proc. Natl. Acad. Sci. U.S.A., 2006;103(31):11784-9. 2006 [PMID: 16864778] (ICC, Mouse)
• Ramos CD, Fernandes KS, Canetti C, Teixeira MM, Silva JS, Cunha FQ Neutrophil recruitment in immunized mice depends on MIP-2 inducing the sequential release of MIP-1alpha, TNF-alpha and LTB(4). Eur. J. Immunol., 2006;36(8):2025-34. 2006 [PMID: 16856209] (ELISA Development, Mouse)
• Osuchowski MF, Welch K, Siddiqui J, Remick DG Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality. J. Immunol., 2006;177(3):1967-74. 2006 [PMID: 16849510] (ELISA Development, Mouse)
• Galic Z, Kitchen SG, Kacena A, Subramanian A, Burke B, Cortado R, Zack JA T lineage differentiation from human embryonic stem cells. Proc. Natl. Acad. Sci. U.S.A., 2006;103(31):11742-7. 2006 [PMID: 16844782] (ICC, Mouse)
• Gao H, Hoesel LM, Guo RF, Rancilio NJ, Sarma JV, Ward PA Adenoviral-mediated overexpression of SOCS3 enhances IgG immune complex-induced acute lung injury. J. Immunol., 2006;177(1):612-20. 2006 [PMID: 16785559] (ELISA Development, Mouse)
• Murata T, Tsuboi M, Hikita K, Kaneda N Protective effects of neurotrophic factors on tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis of murine adrenal chromaffin cell line tsAM5D. J. Biol. Chem., 2006;281(32):22503-16. 2006 [PMID: 16772303] (Neutralization, Mouse)
• An HJ, Jeong HJ, Um JY, Kim HM, Hong SH Glechoma hederacea inhibits inflammatory mediator release in IFN-gamma and LPS-stimulated mouse peritoneal macrophages. J Ethnopharmacol, 2006;106(3):418-24. 2006 [PMID: 16530364] (ELISA Development, Mouse)
• Butovsky O, Ziv Y, Schwartz A, Landa G, Talpalar AE, Pluchino S, Martino G, Schwartz M Microglia activated by IL-4 or IFN-gamma differentially induce neurogenesis and oligodendrogenesis from adult stem/progenitor cells. Mol. Cell. Neurosci., 2006;31(1):149-60. 2006 [PMID: 16297637] (Neutralization, Mouse)
• Mills SJ, Ashworth JJ, Gilliver SC, Hardman MJ, Ashcroft GS The sex steroid precursor DHEA accelerates cutaneous wound healing via the estrogen receptors. J. Invest. Dermatol., 2005;125(5):1053-62. 2005 [PMID: 16297209] (Western Blot, Mouse)
• Polavarapu R, Gongora MC, Winkles JA, Yepes M Tumor necrosis factor-like weak inducer of apoptosis increases the permeability of the neurovascular unit through nuclear factor-kappa B pathway activation. J. Neurosci., 2005;25(44):10094-100. 2005 [PMID: 16267216] (Neutralization, Mouse)
• Osuchowski MF, Siddiqui J, Copeland S, Remick DG Sequential ELISA to profile multiple cytokines from small volumes. J. Immunol. Methods, 2005;302(1):172-81. 2005 [PMID: 16023134] (ELISA Development, Mouse)
• Ellaban E, Bolgos G, Remick D Selective macrophage suppression during sepsis. Cell. Immunol., 2004;231(1):103-11. 2004 [PMID: 15919375] (ELISA Development, Mouse)
• Gonzalez de Aguilar JL, Dupuis L, Larmet Y Antibody-bound beta-amyloid precursor protein stimulates the production of tumor necrosis factor-alpha and monocyte chemoattractant protein-1 by cortical neurons. Neurobiol. Dis., 2005;19(1):129-41. 2005 [PMID: 15837568] (Western Blot, Mouse)
• Jeyaseelan S, Chu HW, Young SK, Worthen GS Transcriptional profiling of lipopolysaccharide-induced acute lung injury. Infect. Immun., 2004;72(12):7247-56. 2004 [PMID: 15557650] (ELISA Development, Mouse)
• LaBue M, Colburn KK, Green LM Thyrocytes isolated from autoimmune-diseased thyroids secrete soluble tumor necrosis factor-R1 that is related to their elevated protein kinase C activity. Thyroid, 2004;14(4):249-62. 2004 [PMID: 15142358] (Immunoprecipitation, Mouse)
• Tezel G, Yang X, Yang J, Wax MB Role of tumor necrosis factor receptor-1 in the death of retinal ganglion cells following optic nerve crush injury in mice. Brain Res., 2004;996(2):202-12. 2004 [PMID: 14697498] (IHC, Rat)
• De SK, Devadas K, Notkins AL Elevated levels of tumor necrosis factor alpha (TNF-alpha) in human immunodeficiency virus type 1-transgenic mice: prevention of death by antibody to TNF-alpha. J. Virol., 2002;76(22):11710-4. 2002 [PMID: 12388730] (Neutralization, Mouse)

FAQs for TNF-alpha Antibody (AF-410-NA). (Showing 1 - 4 of 4 FAQs).

1. I need to know about TNF-alpha antibody kits for mice.
   • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
   • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
   • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
   • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

Bioinformatics

• Gene Symbol: TNF
• Uniprot:
  • P06804()