Western Blot: Human TLR3 Stable Cell Line [NBP2-26267] - Analysis of TLR3 expression in the TLR3 stable cell line using an HA antibody (20 ug total protein/lane). Legend. Vect: Vector control stable cell line; TLR3: ...read more
Flow Cytometry: Human TLR3 Stable Cell Line [NBP2-26267] - Analysis of TLR3 expression in the TLR3 stable cell line. Intracellular expression of TLR3 in the TLR3 stable cell line was analyzed by flow cytometry using a ...read more
Functional: Human TLR3 Stable Cell Line [NBP2-26267] - Functional analysis of the TLR3 stable cell line. The assay was performed using the NF-kB SEAPorter™ Assay Kit. The Vector control stable cell line and TLR3 ...read more
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Human TLR3 Stable Cell Line Summary
The TLR3 stable cell line can be used for TLR3 flow cytometric calibration and detection control as well as TLR3-dependent functional assays. TLR3 expression in this stable cell line has been validated by Western blotting (Fig. 1) and flow cytometry (Fig. 2). Functional activity of this stable cell line has been validated using the NF-kB/SEAPorte™ Assay Kit (NBP2-25286 Fig. 3).
Contents: 3~4 x 10^6 cells
Biosafety Level: 2
The TLR3 stable cell line is a stably transfected cell line which expresses full-length human Toll-like receptor 3 (TLR3) with an N-terminal HA tag.
Assume all cultures are hazardous since they may harbor latent viruses or other organisms that are uncharacterized. The following safety precautions should be observed. - Use pipette aids to prevent ingestion and keep aerosols down to a minimum. - No eating, drinking or smoking while handling the stable line. - Wash hands after handling the stable line and before leaving the lab. - Decontaminate work surface with disinfectant or 70% ethanol before and after working with cells. - All waste should be considered hazardous. - Dispose of all liquid waste after each experiment and treat with bleach.
Alternate Names for Human TLR3 Stable Cell Line
toll-like receptor 3
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Reporter Cell Lines are guaranteed for 1 year from date of receipt.
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