Human TLR3 Stable Cell Line

Images

 
Western Blot: Human TLR3 Stable Cell Line [NBP2-26267] - Analysis of TLR3 expression in the TLR3 stable cell line using an HA antibody (20 ug total protein/lane). Legend. Vect: Vector control stable cell line; TLR3: ...read more
Flow Cytometry: Human TLR3 Stable Cell Line [NBP2-26267] - Analysis of TLR3 expression in the TLR3 stable cell line. Intracellular expression of TLR3 in the TLR3 stable cell line was analyzed by flow cytometry using a ...read more
Functional: Human TLR3 Stable Cell Line [NBP2-26267] - Functional analysis of the TLR3 stable cell line. The assay was performed using the NF-kB SEAPorter™ Assay Kit. The Vector control stable cell line and TLR3 ...read more

Product Details

Summary
Host
HEK293

Order Details

Human TLR3 Stable Cell Line Summary

Description
The TLR3 stable cell line can be used for TLR3 flow cytometric calibration and detection control as well as TLR3-dependent functional assays. TLR3 expression in this stable cell line has been validated by Western blotting (Fig. 1) and flow cytometry (Fig. 2). Functional activity of this stable cell line has been validated using the NF-kB/SEAPorte™ Assay Kit (NBP2-25286 Fig. 3).

Contents: 3~4 x 10^6 cells
Biosafety Level: 2
Immunogen
The TLR3 stable cell line is a stably transfected cell line which expresses full-length human Toll-like receptor 3 (TLR3) with an N-terminal HA tag.
Target Species
Human
Specificity
TLR3
Selection Agent
Blasticidin
Growth Properties
AdherentMorphology: Epithelial
RCL Type
Stable Cell Line
Host
HEK293
Gene
TLR3

Applications/Dilutions

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Publications
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NBP2-26267 in the following applications:

Packaging, Storage & Formulations

Storage
Store in gas phase of liquid nitrogen.
Reconstitution Instructions
Complete Growth Medium: DMEM with 4.5 g/L glucose + 10% FBS + 4 mM L-glutamine + 1 mM sodium pyruvate + 100 units/ml penicillin + 0.1 mg/ml streptomycin + 10 ug/ml blasticidin.

Details for Array

Type
Cell

Notes

Assume all cultures are hazardous since they may harbor latent viruses or other organisms that are uncharacterized. The following safety precautions should be observed.
- Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
- No eating, drinking or smoking while handling the stable line.
- Wash hands after handling the stable line and before leaving the lab.
- Decontaminate work surface with disinfectant or 70% ethanol before and after working with cells.
- All waste should be considered hazardous.
- Dispose of all liquid waste after each experiment and treat with bleach.

Alternate Names for Human TLR3 Stable Cell Line

  • CD283 antigen
  • CD283
  • TLR3
  • toll-like receptor 3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Reporter Cell Lines are guaranteed for 1 year from date of receipt.

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Publications for TLR3 Reporter Cell Line (NBP2-26267)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: ELISA, ICC/IF, WB.


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ELISA
(1)
ICC/IF
(1)
WB
(1)
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Product General Protocols

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Blogs on TLR3.

The role of STING/TMEM173 in gamma and encephalitis Herpes Simplex Virus (HSV)
Stimulator of interferon genes (STING), also known as TMEM173, promotes the production of the interferon’s IFN-alpha and IFN-beta.  STING possesses three functional domains: a cytoplasmic C-terminal tail, a central globular domain, and four N-...  Read full blog post.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

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Bioinformatics

Gene Symbol TLR3
Entrez