Tissue alpha-L-Fucosidase/FUCA1 Antibody (1D4) - Azide and BSA Free

Western Blot: Tissue alpha-L-Fucosidase/FUCA1 Antibody (1D4) [H00002517-M01] - Analysis of FUCA1 expression in transfected 293T cell line by FUCA1 monoclonal antibody (M01), clone 1D4. Lane 1: FUCA1 transfected lysate(53.2 KDa). Lane 2: Non-transfected lysate.

Western Blot: Tissue alpha-L-Fucosidase/FUCA1 Antibody (1D4) [H00002517-M01] - FUCA1 monoclonal antibody (M01), clone 1D4 Western Blot analysis of FUCA1 expression in K-562 ( Cat # L009V1 ).

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 1D4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Tissue alpha-L-Fucosidase/FUCA1 Antibody (1D4) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: FUCA1 (AAH17338, 23 a.a. ~ 461 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VRRAQPPRRYTPDWPSLDSRPLPAWFDEAKFGVFIHWGVFSVPAWGSEWFWWHWQGEGRPQYQRFMRDNYPPGFSYADFGPQFTARFFHPEEWADLFQAAGAKYVVLTTKHHEGFTNWPSPVSWNWNSKDVGPHRDLVGELGTALRKRNIRYGLYHSLLEWFHPLYLLDKKNGFKTQHFVSAKTMPELYDLVNSYKPDLIWSDGEWECPDTYWNSTNFLSWLYNDSPVKDEVVVNDRWGQNCSCHHGGYYNCEDKFKPQSLPDHKWEMCTSIDKFSWGYRRDMALSDVTEESEIISELVQTVSLGGNYLLNIGPTKDGLIVPIFQERLLAVGKWLSINGEAIYASKPWRVQWEKNTTSVWYTSKGSAVYAIFLHWPENGVLNLESPITTSTTKITMLGIQGDLKWSTDPDKGLFISLPQLPPSAVPAEFAWTIKLTGVK
• Specificity: FUCA1 - fucosidase, alpha-L- 1, tissue
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: FUCA1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA. Use in Immunohistochemistry reported in scientific literature (PMID: 26204487).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Tissue alpha-L-Fucosidase/FUCA1 Antibody (1D4) - Azide and BSA Free

• Alpha-L-fucosidase 1
• Alpha-L-fucosidase I
• Alpha-L-fucoside fucohydrolase 1
• EC 3.2.1
• EC 3.2.1.51
• FUCA
• FUCA1
• fucosidase, alpha-L- 1, tissue
• Tissue alphaLFucosidase
• Tissue alpha-L-Fucosidase

Background

Fucosidosis is an autosomal recessive lysosomal storage disease caused by defective alpha-L-fucosidase with accumulation of fucose in the tissues. Different phenotypes include clinical features such as neurologic deterioration, growth retardation, visceromegaly, and seizures in a severe early form; coarse facial features, angiokeratoma corporis diffusum, spasticity and delayed psychomotor development in a longer surviving form; and an unusual spondylometaphyseoepiphyseal dysplasia in yet another form.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Tissue alpha-L-Fucosidase/FUCA1 Antibody (H00002517-M01)(1)

Publication using H00002517-M01

• Cheng TC, Tu SH, Chen LC et al. Down-regulation of alpha-L-fucosidase 1 expression confers inferior survival for triple-negative breast cancer patients by modulating the glycosylation status of the tumor cell surface. Oncotarget 2015-08-28 [PMID: 26204487] (IF/IHC)

Bioinformatics

• Gene Symbol: FUCA1
• Entrez:
  • 2517(Human)
• OMIM:
  • 230000(Human)
• Uniprot:
  • AAH17338(Human)