TIM-3 Antibody (344823) [Unconjugated]

Human peripheral blood monocytes were stained with Rat Anti-Human TIM‑3 Monoclonal Antibody (Catalog # MAB2365, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 344823
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

TIM-3 Antibody (344823) [Unconjugated] Summary

• Additional Information: Clone 344823 was used by HLDA to establish CD designation
• Immunogen: Mouse myeloma cell line NS0-derived recombinant human TIM‑3
  Ser22-Arg200
  Accession # Q8TDQ0.2
• Specificity: Detects human TIM‑3 in direct ELISAs. Does not cross-react with recombinant human (rh) TIM‑1, rhTIM‑4, recombinant mouse (rm) TIM‑1, rmTIM‑2, rmTIM‑3, rmTIM‑5, or rmTIM‑6.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: HAVCR2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-reported
  • Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TIM-3 Antibody (344823) [Unconjugated]

• CD366
• FLJ14428
• HAVCR2
• HAVcr-2
• hepatitis A virus cellular receptor 2
• kidney injury molecule-3
• KIM-3
• SPTCL
• T cell immunoglobulin mucin 3
• T cell immunoglobulin mucin-3
• T-cell immunoglobulin and mucin domain-containing protein 3
• TIM 3
• TIM3 T-cell membrane protein 3
• TIM3
• TIM-3
• TIMD3
• TIMD-3
• TIMD3KIM-3

Background

TIM-3 (T cell Immunoglobulin and Mucin domain-3) is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk (1-3). Mature human TIM-3 consists of a 181 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 78 aa cytoplasmic tail (4). An alternately spliced isoform is truncated following a short substitution after the Ig-like domain. Within the ECD, human TIM-3 shares 58% aa sequence identity with mouse and rat TIM-3. TIM-3 is expressed on the surface of effector T cells (CD4⁺ Th1 and CD8⁺ Tc1) but not on helper T cells (CD4⁺ Th2 and CD8⁺ Tc2) (4, 5). In chronic inflammation, autoimmune disorders, and some cancers, TIM-3 is upregulated on several other hematopoietic cell types. The Ig domain of TIM-3 interacts with a ligand on resting but not activated Th1 and Th2 cells (5, 6). The glycosylated Ig domain of TIM-3 binds cell-associated galectin-9. This induces TIM-3 Tyr phosphorylation and proapoptotic signaling (7). TIM-3 functions as a negative regulator of Th1 cell activity. Its blockade results in increased IFN-gamma production, Th1 cell proliferation and cytotoxicity (5, 6, 8), regulatory T cell development (5), and increases in macrophage and neutrophil infiltration into sites of inflammation (9).

1. Anderson, A.C. and D.E. Anderson (2006) Curr. Opin. Immunol. 18:665.
2. Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.
3. Meyers, J.H. et al. (2005) Trends Mol. Med. 11:362.
4. Monney, L. et al. (2002) Nature 415:536.
5. Sanchez-Fueyo, A. et al. (2003) Nat. Immunol. 4:1093.
6. Sabatos, C.A. et al. (2003) Nat. Immunol. 4:1102.
7. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
8. Koguchi, K. et al. (2006) J. Exp. Med. 203:1413.
9. Frisancho-Kiss, S. et al. (2006) J. Immunol. 176:6411.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TIM-3 Antibody (MAB2365)(15)

Publications using MAB2365

• Jessica Pinkert, Hans-Henning Boehm, Mark Trautwein, Wolf-Dietrich Doecke, Florian Wessel, Yingzi Ge et al. T cell-mediated elimination of cancer cells by blocking CEACAM6–CEACAM1 interaction OncoImmunology 2022-12-31 [PMID: 35141051]
• MA Smith, CC Chiang, K Zerrouki, S Rahman, WI White, K Streicher, WA Rees, A Schiffenba, LG Rider, FW Miller, Z Manna, S Hasni, MJ Kaplan, R Siegel, D Sinibaldi, MA Sanjuan, KA Casey Using the circulating proteome to assess type I interferon activity in systemic lupus erythematosus Sci Rep, 2020-03-10;10(1):4462. 2020-03-10 [PMID: 32157125] (Flow Cytometry, Human)
• H Sun, J Xu, Q Huang, M Huang, K Li, K Qu, H Wen, R Lin, M Zheng, H Wei, W Xiao, R Sun, Z Tian, C Sun Reduced CD160 expression contributes to impaired NK cell function and poor clinical outcomes in patients with HCC Cancer Res., 2018-09-19;0(0):. 2018-09-19 [PMID: 30232222] (Flow Cytometry, Human)
• CA Klebanoff, JG Crompton, AJ Leonardi, TN Yamamoto, SS Chandran, RL Eil, M Sukumar, SK Vodnala, J Hu, Y Ji, D Clever, MA Black, D Gurusamy, MJ Kruhlak, P Jin, DF Stroncek, L Gattinoni, SA Feldman, NP Restifo Inhibition of AKT signaling uncouples T cell differentiation from expansion for receptor-engineered adoptive immunotherapy JCI Insight, 2017-12-07;2(23):. 2017-12-07 [PMID: 29212954] (Flow Cytometry, Mouse)
• C Stecher, C Battin, J Leitner, M Zettl, K Grabmeier-, C Höller, GJ Zlabinger, P Steinberge PD-1 Blockade Promotes Emerging Checkpoint Inhibitors in Enhancing T Cell Responses to Allogeneic Dendritic Cells Front Immunol, 2017-05-22;8(0):572. 2017-05-22 [PMID: 28588576] (Neutralization, Human)
• JA Schwartz, KL Clayton, S Mujib, H Zhang, AK Rahman, J Liu, FY Yue, E Benko, C Kovacs, MA Ostrowski Tim-3 is a Marker of Plasmacytoid Dendritic Cell Dysfunction during HIV Infection and Is Associated with the Recruitment of IRF7 and p85 into Lysosomes and with the Submembrane Displacement of TLR9 J. Immunol, 2017-03-06;0(0):. 2017-03-06 [PMID: 28264968] (Neutralization, Human)
• PD-1/PD-L1 blockade enhances T cell activity and antitumor efficacy of imatinib in gastrointestinal stromal tumors Clin Cancer Res, 2016-07-28;0(0):. 2016-07-28 [PMID: 27470968] (Flow Cytometry, Human)
• Human CAR T cells with cell-intrinsic PD-1 checkpoint blockade resist tumor-mediated inhibition J Clin Invest, 2016-07-25;126(8):3130-44. 2016-07-25 [PMID: 27454297] (Human)
• Yasmina Noubia Abdiche, Yik Andy Yeung, Javier Chaparro-Riggers, Ishita Barman, Pavel Strop, Sherman Michael Chin et al. The neonatal Fc receptor (FcRn) binds independently to both sites of the IgG homodimer with identical affinity mAbs 2015-03-04 [PMID: 25658443]
• Clayton K, Douglas-Vail M, Nur-ur Rahman A, Medcalf K, Xie I, Chew G, Tandon R, Lanteri M, Norris P, Deeks S, Ndhlovu L, Ostrowski M Soluble T cell immunoglobulin mucin domain 3 is shed from CD8+ T cells by the sheddase ADAM10, is increased in plasma during untreated HIV infection, and correlates with HIV disease progression. J Virol, 2015-01-21;89(7):3723-36. 2015-01-21 [PMID: 25609823] (Human)
• John A. Hansen, Samir M. Hanash, Laura Tabellini, Chris Baik, Richard L. Lawler, Bryan M. Grogan et al. A Novel Soluble Form of Tim-3 Associated with Severe Graft-versus-Host Disease Biology of Blood and Marrow Transplantation 2013-09-01 [PMID: 23791624]
• Golden-Mason L, McMahan R, Strong M, Reisdorph R, Mahaffey S, Palmer B, Cheng L, Kulesza C, Hirashima M, Niki T, Rosen H Galectin-9 functionally impairs natural killer cells in humans and mice. J Virol, 2013-02-13;87(9):4835-45. 2013-02-13 [PMID: 23408620] (Neutralization, Human)
• Hertoghs KM, Moerland PD, van Stijn A, Remmerswaal EB, Yong SL, van de Berg PJ, van Ham SM, Baas F, ten Berge IJ, van Lier RA Molecular profiling of cytomegalovirus-induced human CD8+ T cell differentiation. J. Clin. Invest., 2010-11-01;120(11):4077-90. 2010-11-01 [PMID: 20921622] (Flow Cytometry, Human)
• Vali B, Jones RB, Sakhdari A HCV-specific T cells in HCV/HIV co-infection show elevated frequencies of dual Tim-3/PD-1 expression that correlate with liver disease progression. Eur. J. Immunol., 2010-09-01;40(9):2493-505. 2010-09-01 [PMID: 20623550] (Flow Cytometry, Human)
• Bi S, Earl LA, Jacobs L, Baum LG Structural features of galectin-9 and galectin-1 that determine distinct T cell death pathways. J. Biol. Chem., 2008-02-07;283(18):12248-58. 2008-02-07 [PMID: 18258591] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: HAVCR2
• Uniprot:
  • Q8TDQ0.2()