TIM-3 Antibody (344823) [PerCP (Peridinin-chlorophyll Protein Complex)]

Human peripheral blood monocytes were stained with Rat Anti-Human TIM-3 PerCP-conjugated Monoclonal Antibody (Catalog # FAB2365C, filled histogram) or isotype control antibody (Catalog # IC006C, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 344823
• Clonality: Monoclonal
• Host: Rat
• Conjugate: PerCP (Peridinin-chlorophyll Protein Complex)

TIM-3 Antibody (344823) [PerCP (Peridinin-chlorophyll Protein Complex)] Summary

• Additional Information: Clone 344823 was used by HLDA to establish CD designation
• Immunogen: Mouse myeloma cell line NS0-derived recombinant human TIM‑3
  Ser22-Arg200
  Accession # Q8TDQ0.2
• Specificity: Detects human TIM‑3 in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) TIM‑1, rhTIM‑4, recombinant mouse (rm) TIM‑1, rmTIM‑2, rmTIM‑3, rmTIM‑5, or rmTIM‑6.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: HAVCR2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TIM-3 Antibody (344823) [PerCP (Peridinin-chlorophyll Protein Complex)]

• CD366
• FLJ14428
• HAVCR2
• HAVcr-2
• hepatitis A virus cellular receptor 2
• kidney injury molecule-3
• KIM-3
• SPTCL
• T cell immunoglobulin mucin 3
• T cell immunoglobulin mucin-3
• T-cell immunoglobulin and mucin domain-containing protein 3
• TIM 3
• TIM3 T-cell membrane protein 3
• TIM3
• TIM-3
• TIMD3
• TIMD-3
• TIMD3KIM-3

Background

TIM-3 (T cell immunoglobulin and mucin domain-3), also known as HAVCR2, is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk region (1, 2). Mature human TIM-3 consists of a 181 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 78 aa cytoplasmic tail (3). An alternatively spliced isoform is truncated within the mucin-like stalk. Within the ECD, human TIM-3 shares 58% aa sequence identity with mouse and rat TIM-3. TIM-3 is up‑regulated on several populations of activated myeloid cells (macrophage, monocyte, dendritic cell, microglia, mast cell) and T cells (Th1, CD8⁺, NK, Treg) (3-10). Its binding to Galectin-9 induces a range of immunosuppressive functions which enhance immune tolerance and inhibit anti-tumor immunity (11). TIM-3 ligation attenuates CD8⁺ and Th1 cell responses (11-13) and promotes the activity of Treg and myeloid derived suppressor cells (8, 11, 13, 14). In addition, dendritic cell-expressed TIM-3 dampens inflammation by enabling the phagocytosis of apoptotic cells and the cross-presentation of apoptotic cell antigens (4). It also binds the alarmin HMGB1, thereby preventing the activation of TLRs in response to released tumor cell DNA (7). TIM-3 interactions with Galectin-9 can alternatively trigger immune stimulatory effects, such as the coactivation of NK cell cytotoxicity (10).

1. Sakuishi, K. et al. (2011) Trends Immunol. 32:345.
2. Anderson, A.C. (2012) Curr. Opin. Immunol. 24:213.
3. Monney, L. et al. (2002) Nature 415:536.
4. Nakayama, M. et al. (2009) Blood 113:3821.
5. Anderson, A.C. et al. (2007) Science 318:1141.
6. Wiener, Z. et al. (2007) J. Invest. Dermatol. 127:906.
7. Chiba, S. et al. (2012) Nat. Immunol. 13:832.
8. Sanchez-Fueyo, A. et al. (2003) Nat. Immunol. 4:1093.
9. Ndhlovu, L.C. et al. (2012) Blood 119:3734.
10. Gleason, M.K. et al. (2012) Blood 119:3064.
11. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
12. Sakhdari, A. et al. (2012) PLoS ONE 7:e40146.
13. Sabatos, C.A. et al. (2003) Nat. Immunol. 4:1102.
14. Dardalhon, V. et al. (2010) J. Immunol. 185:1383.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TIM-3 Antibody (FAB2365C)(4)

Publications using FAB2365C

• J Akagi, H Baba Hydrogen gas activates coenzyme Q10 to restore exhausted CD8+ T cells, especially PD-1+Tim3+terminal CD8+ T cells, leading to better nivolumab outcomes in patients with lung cancer Oncology Letters, 2020-09-18;20(5):258. 2020-09-18 [PMID: 32994821] (Flow Cytometry, Human)
• J Mateus, E Pérez-Antó, P Lasso, A Egui, N Roa, B Carrilero, JM González, MC Thomas, CJ Puerta, MC López, A Cuéllar Antiparasitic Treatment Induces an Improved CD8(+) T Cell Response in Chronic Chagasic Patients J. Immunol, 2017-03-03;0(0):. 2017-03-03 [PMID: 28258194] (Flow Cytometry, Human)
• Lasso P, Mateus J, Pavia P, Rosas F, Roa N, Thomas M, Lopez M, Gonzalez J, Puerta C, Cuellar A Inhibitory Receptor Expression on CD8+ T Cells Is Linked to Functional Responses against Trypanosoma cruzi Antigens in Chronic Chagasic Patients. J Immunol, 2015-09-18;195(8):3748-58. 2015-09-18 [PMID: 26385520] (Flow Cytometry, Human)
• Kared H, Saeed S, Klein M, Shoukry N CD127 expression, exhaustion status and antigen specific proliferation predict sustained virologic response to IFN in HCV/HIV co-infected individuals. PLoS ONE, 2014-07-09;9(7):e101441. 2014-07-09 [PMID: 25007250] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: HAVCR2
• Uniprot:
  • Q8TDQ0.2()