TIM-3 Antibody (344823) [PerCP (Peridinin-chlorophyll Protein Complex)]

• Applications: Flow
• Clone: 344823
• Clonality: Monoclonal
• Host: Rat
• Conjugate: PerCP (Peridinin-chlorophyll Protein Complex)

TIM-3 Antibody (344823) [PerCP (Peridinin-chlorophyll Protein Complex)] Summary

• Additional Information: Clone 344823 was used by HLDA to establish CD designation
• Immunogen: Mouse myeloma cell line NS0-derived recombinant human TIM‑3
  Ser22-Arg200
  Accession # Q8TDQ0.2
• Specificity: Detects human TIM‑3 in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) TIM‑1, rhTIM‑4, recombinant mouse (rm) TIM‑1, rmTIM‑2, rmTIM‑3, rmTIM‑5, or rmTIM‑6.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: HAVCR2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

TIM-3 (T cell immunoglobulin and mucin domain-3), also known as HAVCR2, is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk region (1, 2). Mature human TIM-3 consists of a 181 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 78 aa cytoplasmic tail (3). An alternatively spliced isoform is truncated within the mucin-like stalk. Within the ECD, human TIM-3 shares 58% aa sequence identity with mouse and rat TIM-3. TIM-3 is up‑regulated on several populations of activated myeloid cells (macrophage, monocyte, dendritic cell, microglia, mast cell) and T cells (Th1, CD8⁺, NK, Treg) (3-10). Its binding to Galectin-9 induces a range of immunosuppressive functions which enhance immune tolerance and inhibit anti-tumor immunity (11). TIM-3 ligation attenuates CD8⁺ and Th1 cell responses (11-13) and promotes the activity of Treg and myeloid derived suppressor cells (8, 11, 13, 14). In addition, dendritic cell-expressed TIM-3 dampens inflammation by enabling the phagocytosis of apoptotic cells and the cross-presentation of apoptotic cell antigens (4). It also binds the alarmin HMGB1, thereby preventing the activation of TLRs in response to released tumor cell DNA (7). TIM-3 interactions with Galectin-9 can alternatively trigger immune stimulatory effects, such as the coactivation of NK cell cytotoxicity (10).

1. Sakuishi, K. et al. (2011) Trends Immunol. 32:345.
2. Anderson, A.C. (2012) Curr. Opin. Immunol. 24:213.
3. Monney, L. et al. (2002) Nature 415:536.
4. Nakayama, M. et al. (2009) Blood 113:3821.
5. Anderson, A.C. et al. (2007) Science 318:1141.
6. Wiener, Z. et al. (2007) J. Invest. Dermatol. 127:906.
7. Chiba, S. et al. (2012) Nat. Immunol. 13:832.
8. Sanchez-Fueyo, A. et al. (2003) Nat. Immunol. 4:1093.
9. Ndhlovu, L.C. et al. (2012) Blood 119:3734.
10. Gleason, M.K. et al. (2012) Blood 119:3064.
11. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
12. Sakhdari, A. et al. (2012) PLoS ONE 7:e40146.
13. Sabatos, C.A. et al. (2003) Nat. Immunol. 4:1102.
14. Dardalhon, V. et al. (2010) J. Immunol. 185:1383.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HAVCR2