TIM-3 Antibody (344823) [Phycoerythrin]

Human peripheral blood monocytes were stained with Rat Anti-Human TIM-3 PE-conjugated Monoclonal Antibody (Catalog # FAB2365P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Upregulation of immune checkpoint ligands on T cells after dendritic cell stimulation. T cells of 7–14 healthy donor were cocultured with autologous TLR-3-DCs pulsed with CMV, EBV, influenza, tetanus (CEFT) peptide pool or with CEFT peptide pool alone. Expression of various inhibitory checkpoint molecules was analyzed by flow cytometry. The percentage of positive cells is presented as box-and-whisker plots for CD4+(A) and for CD8+(B) T cells. *p < 0.05; **p < 0.01. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fimmu.2018.00385/full), licensed under a CC-BY license. Not internally tested by R&D Systems.

Upregulation of immune checkpoint ligands on T cells after dendritic cell stimulation. T cells of 7–14 healthy donor were cocultured with autologous TLR-3-DCs pulsed with CMV, EBV, influenza, tetanus (CEFT) peptide pool or with CEFT peptide pool alone. Expression of various inhibitory checkpoint molecules was analyzed by flow cytometry. The percentage of positive cells is presented as box-and-whisker plots for CD4+(A) and for CD8+(B) T cells. *p < 0.05; **p < 0.01. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fimmu.2018.00385/full), licensed under a CC-BY license. Not internally tested by R&D Systems.

Gating strategy for phenotyping IFN-gamma secreting T cells in response to stimulation.The frequencies of Tim-3+ and Tim-3− IFN-gamma secreting CD4+ and CD8+ T cells were measured in PBMC collected at day 30 post-index from 6 HLA-A02 WNV-infected donors and incubated with or without anti-CD3/anti-CD28 mAbs, WNV peptide pool, and SVG9 tetramer. Tim-3− and Tim-3+ CD4+ and CD8+ T cells were analyzed for IFN-gamma secretion. The gates were set using fluorescence minus one controls. Dot-plots for CD8+ T cells from all 6 WNV+ subjects in different stimulation conditions are shown. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0092134), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: Flow
• Clone: 344823
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Phycoerythrin

TIM-3 Antibody (344823) [Phycoerythrin] Summary

• Additional Information: Clone 344823 was used by HLDA to establish CD designation
• Immunogen: Mouse myeloma cell line NS0-derived recombinant human TIM‑3
  Ser22-Arg200
  Accession # Q8TDQ0.2
• Specificity: Detects human TIM‑3 in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) TIM‑1, rhTIM‑4, recombinant mouse (rm) TIM‑1, rmTIM‑2, rmTIM‑3, rmTIM‑5, or rmTIM‑6.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: HAVCR2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TIM-3 Antibody (344823) [Phycoerythrin]

• CD366
• FLJ14428
• HAVCR2
• HAVcr-2
• hepatitis A virus cellular receptor 2
• kidney injury molecule-3
• KIM-3
• SPTCL
• T cell immunoglobulin mucin 3
• T cell immunoglobulin mucin-3
• T-cell immunoglobulin and mucin domain-containing protein 3
• TIM 3
• TIM3 T-cell membrane protein 3
• TIM3
• TIM-3
• TIMD3
• TIMD-3
• TIMD3KIM-3

Background

TIM-3 (T cell immunoglobulin and mucin domain-3), also known as HAVCR2, is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk region (1, 2). Mature human TIM-3 consists of a 181 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 78 aa cytoplasmic tail (3). An alternatively spliced isoform is truncated within the mucin-like stalk. Within the ECD, human TIM-3 shares 58% aa sequence identity with mouse and rat TIM-3. TIM-3 is up‑regulated on several populations of activated myeloid cells (macrophage, monocyte, dendritic cell, microglia, mast cell) and T cells (Th1, CD8⁺, NK, Treg) (3-10). Its binding to Galectin-9 induces a range of immunosuppressive functions which enhance immune tolerance and inhibit anti-tumor immunity (11). TIM-3 ligation attenuates CD8⁺ and Th1 cell responses (11-13) and promotes the activity of Treg and myeloid derived suppressor cells (8, 11, 13, 14). In addition, dendritic cell-expressed TIM-3 dampens inflammation by enabling the phagocytosis of apoptotic cells and the cross-presentation of apoptotic cell antigens (4). It also binds the alarmin HMGB1, thereby preventing the activation of TLRs in response to released tumor cell DNA (7). TIM-3 interactions with Galectin-9 can alternatively trigger immune stimulatory effects, such as the coactivation of NK cell cytotoxicity (10).

1. Sakuishi, K. et al. (2011) Trends Immunol. 32:345.
2. Anderson, A.C. (2012) Curr. Opin. Immunol. 24:213.
3. Monney, L. et al. (2002) Nature 415:536.
4. Nakayama, M. et al. (2009) Blood 113:3821.
5. Anderson, A.C. et al. (2007) Science 318:1141.
6. Wiener, Z. et al. (2007) J. Invest. Dermatol. 127:906.
7. Chiba, S. et al. (2012) Nat. Immunol. 13:832.
8. Sanchez-Fueyo, A. et al. (2003) Nat. Immunol. 4:1093.
9. Ndhlovu, L.C. et al. (2012) Blood 119:3734.
10. Gleason, M.K. et al. (2012) Blood 119:3064.
11. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
12. Sakhdari, A. et al. (2012) PLoS ONE 7:e40146.
13. Sabatos, C.A. et al. (2003) Nat. Immunol. 4:1102.
14. Dardalhon, V. et al. (2010) J. Immunol. 185:1383.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TIM-3 Antibody (FAB2365P)(37)

Publications using FAB2365P

• Urak, R;Gittins, B;Soemardy, C;Grepo, N;Goldberg, L;Maker, M;Shevchenko, G;Davis, A;Li, S;Scott, T;Morris, KV;Forman, SJ;Wang, X; Evaluation of the Elements of Short Hairpin RNAs in Developing shRNA-Containing CAR T Cells Cancers 2023-05-20 [PMID: 37345185] (Flow Cytometry, Human)
• Courtney M. Smith, Alice Li, Nithya Krishnamurthy, Mark A. Lemmon Phosphatidylserine binding directly regulates TIM-3 function Biochemical Journal 2021-09-17 [PMID: 34435619]
• MJ Lee, SJ Yun, B Lee, E Jeong, G Yoon, K Kim, S Park Association of TIM-3 expression with glucose metabolism in Jurkat T cells BMC Immunol., 2020-08-20;21(1):48. 2020-08-20 [PMID: 32819283] (Flow Cytometry, Human)
• M Meggyes, L Szereday, N Bohonyi, M Koppan, S Szegedi, A Marics-Kut, M Marton, A Totsimon, B Polgar Different Expression Pattern of TIM-3 and Galectin-9 Molecules by Peripheral and Peritoneal Lymphocytes in Women with and without Endometriosis Int J Mol Sci, 2020-03-28;21(7):. 2020-03-28 [PMID: 32231038] (Flow Cytometry, Human)
• AG Chapuis, DN Egan, M Bar, TM Schmitt, MS McAfee, KG Paulson, V Voillet, R Gottardo, GB Ragnarsson, M Bleakley, CC Yeung, P Muhlhauser, HN Nguyen, LA Kropp, L Castelli, F Wagener, D Hunter, M Lindberg, K Cohen, A Seese, MJ McElrath, N Duerkopp, TA Gooley, PD Greenberg T cell receptor gene therapy targeting WT1 prevents acute myeloid leukemia relapse post-transplant Nat. Med., 2019-06-24;0(0):. 2019-06-24 [PMID: 31235963] (Flow Cytometry, Human)
• T Duhen, R Duhen, R Montler, J Moses, T Moudgil, NF de Miranda, CP Goodall, TC Blair, BA Fox, JE McDermott, SC Chang, G Grunkemeie, R Leidner, RB Bell, AD Weinberg Co-expression of CD39 and CD103 identifies tumor-reactive CD8 T cells in human solid tumors Nat Commun, 2018-07-13;9(1):2724. 2018-07-13 [PMID: 30006565] (Flow Cytometry, Human)
• GJ Roboz, EK Ritchie, Y Dault, L Lam, DC Marshall, NM Cruz, HC Hsu, DC Hassane, PJ Christos, C Ippoliti, JM Scandura, ML Guzman Phase I trial of plerixafor combined with decitabine in newly diagnosed older patients with acute myeloid leukemia Haematologica, 2018-05-03;0(0):. 2018-05-03 [PMID: 29724902] (Flow Cytometry, Human)
• Felix S. Lichtenegger, Maurine Rothe, Frauke M. Schnorfeil, Katrin Deiser, Christina Krupka, Christian Augsberger et al. Targeting LAG-3 and PD-1 to Enhance T Cell Activation by Antigen-Presenting Cells Frontiers in Immunology 2018-02-27 [PMID: 29535740]
• D Zhao, M Guo, B Liu, Q Lin, T Xie, Q Zhang, X Jia, Q Shu, X Liang, L Gao, C Ma Frontline Science: Tim-3-mediated dysfunctional engulfment of apoptotic cells in SLE J. Leukoc. Biol., 2017-07-28;0(0):. 2017-07-28 [PMID: 28754800] (Flow Cytometry, Human)
• M Allard, B Couturaud, L Carretero-, MN Duong, J Schmidt, GC Monnot, P Romero, DE Speiser, M Hebeisen, N Rufer TCR-ligand dissociation rate is a robust and stable biomarker of CD8+ T cell potency JCI Insight, 2017-07-20;2(14):. 2017-07-20 [PMID: 28724801] (Flow Cytometry, Human)
• L Schofield, LJ Ioannidis, S Karl, LJ Robinson, QY Tan, DP Poole, I Betuela, DL Hill, PM Siba, DS Hansen, I Mueller, EM Eriksson Synergistic effect of IL-12 and IL-18 induces TIM3 regulation of ?? T cell function and decreases the risk of clinical malaria in children living in Papua New Guinea BMC Med, 2017-06-15;15(1):114. 2017-06-15 [PMID: 28615061] (Flow Cytometry, Human)
• C Stecher, C Battin, J Leitner, M Zettl, K Grabmeier-, C Höller, GJ Zlabinger, P Steinberge PD-1 Blockade Promotes Emerging Checkpoint Inhibitors in Enhancing T Cell Responses to Allogeneic Dendritic Cells Front Immunol, 2017-05-22;8(0):572. 2017-05-22 [PMID: 28588576] (Flow Cytometry, Human)
• MA Gasper, AC Hesseling, I Mohar, L Myer, T Azenkot, JS Passmore, W Hanekom, MF Cotton, IN Crispe, DL Sodora, HB Jaspan BCG vaccination induces HIV target cell activation in HIV-exposed infants in a randomized trial JCI Insight, 2017-04-06;2(7):e91963. 2017-04-06 [PMID: 28405623] (Flow Cytometry, Human)
• JA Schwartz, KL Clayton, S Mujib, H Zhang, AK Rahman, J Liu, FY Yue, E Benko, C Kovacs, MA Ostrowski Tim-3 is a Marker of Plasmacytoid Dendritic Cell Dysfunction during HIV Infection and Is Associated with the Recruitment of IRF7 and p85 into Lysosomes and with the Submembrane Displacement of TLR9 J. Immunol, 2017-03-06;0(0):. 2017-03-06 [PMID: 28264968] (Flow Cytometry, Human)
• Kongsted P, Borch T, Ellebaek E, Iversen T, Andersen R, Met O, Hansen M, Lindberg H, Sengelov L, Svane I Dendritic cell vaccination in combination with docetaxel for patients with metastatic castration-resistant prostate cancer: A randomized phase II study. Cytotherapy, 2017-02-15;19(4):500-513. 2017-02-15 [PMID: 28215654] (Flow Cytometry, Human)
• Ornella Franzese, Belinda Palermo, Cosmo Di Donna, Isabella Sperduti, Virginia Ferraresi, Helena Stabile et al. Polyfunctional Melan-A-specific tumor-reactive CD8(+) T cells elicited by dacarbazine treatment before peptide-vaccination depends on AKT activation sustained by ICOS OncoImmunology 2016-05-03 [PMID: 27467927]
• Human CAR T cells with cell-intrinsic PD-1 checkpoint blockade resist tumor-mediated inhibition J Clin Invest, 2016-07-25;126(8):3130-44. 2016-07-25 [PMID: 27454297] (Flow Cytometry, Human)
• CD62L+ NKT cells have prolonged persistence and antitumor activity in vivo J Clin Invest, 2016-05-16;0(0):. 2016-05-16 [PMID: 27183388] (Flow Cytometry, Human)
• CD3(bright)CD56(+) T cells associate with pegylated interferon-alpha treatment nonresponse in chronic hepatitis B patients Sci Rep, 2016-05-13;6(0):25567. 2016-05-13 [PMID: 27174425] (Flow Cytometry, Human)
• ZENGJIN LIU, HUAMIN HAN, XIN HE, SHOUWEI LI, CHENXING WU, CHUNJIANG YU et al. Expression of the galectin-9-Tim-3 pathway in glioma tissues is associated with the clinical manifestations of glioma Oncology Letters 2016-03-01 [PMID: 26998085]
• Clark GJ et al. New insights into the phenotype of human dendritic cell populations. Clin Transl Immunology 1/1/2016 [PMID: 26900474]
• Caroline Pabst, Anne Bergeron, Vincent-Philippe Lavallée, Jonathan Yeh, Patrick Gendron, Gudmundur L. Norddahl et al. GPR56 identifies primary human acute myeloid leukemia cells with high repopulating potential in vivo Blood 2016-04-21 [PMID: 26834243]
• Frederiksen J, Buggert M, Noyan K, Nowak P, Sonnerborg A, Lund O, Karlsson A Multidimensional Clusters of CD4+ T Cell Dysfunction Are Primarily Associated with the CD4/CD8 Ratio in Chronic HIV Infection. PLoS ONE, 2015-09-24;10(9):e0137635. 2015-09-24 [PMID: 26402620] (Flow Cytometry, Human)
• Kared H, Saeed S, Klein M, Shoukry N CD127 expression, exhaustion status and antigen specific proliferation predict sustained virologic response to IFN in HCV/HIV co-infected individuals. PLoS ONE, 2014-07-09;9(7):e101441. 2014-07-09 [PMID: 25007250] (Flow Cytometry, Human)
• Meggyes , Matyas, Miko , Eva, Polgar , Beata, Bogar , Barbara, Farkas , Balint, Illes , Zsolt, Szereday , Laszlo Peripheral blood TIM-3 positive NK and CD8+ T cells throughout pregnancy: TIM-3/galectin-9 interaction and its possible role during pregnancy. PLoS ONE, 2014-03-20;9(3):e92371. 2014-03-20 [PMID: 24651720] (Flow Cytometry, Human)
• Lanteri M, Diamond M, Law J, Chew G, Wu S, Inglis H, Wong D, Busch M, Norris P, Ndhlovu L Increased frequency of Tim-3 expressing T cells is associated with symptomatic West Nile virus infection. PLoS ONE, 2014-03-18;9(3):e92134. 2014-03-18 [PMID: 24642562] (Flow Cytometry, Human)
• Herath S, Benlahrech A, Papagatsias T, Athanasopoulos T, Bouzeboudjen Z, Hervouet C, Klavinskis L, Meiser A, Kelleher P, Dickson G, Patterson S Fusion of ubiquitin to HIV gag impairs human monocyte-derived dendritic cell maturation and reduces ability to induce gag T cell responses. PLoS ONE, 2014-02-05;9(2):e88327. 2014-02-05 [PMID: 24505475] (Flow Cytometry, Human)
• Clayton K, Haaland M, Douglas-Vail M, Mujib S, Chew G, Ndhlovu L, Ostrowski M T cell Ig and mucin domain-containing protein 3 is recruited to the immune synapse, disrupts stable synapse formation, and associates with receptor phosphatases. J Immunol, 2013-12-13;192(2):782-91. 2013-12-13 [PMID: 24337741] (ICC, Human)
• Helen T. Michael, Daisuke Ito, Valarie McCullar, Bin Zhang, Jeffrey S. Miller, Jaime F. Modiano Isolation and characterization of canine natural killer cells Veterinary Immunology and Immunopathology 2013-09-15 [PMID: 23876304] (Flow Cytometry, Canine)
• Mansfield A, Nevala W, Lieser E, Leontovich A, Markovic S The immunomodulatory effects of bevacizumab on systemic immunity in patients with metastatic melanoma. Oncoimmunology, 2013-05-01;2(5):e24436. 2013-05-01 [PMID: 23762809] (Flow Cytometry, Human)
• Nebbia G, Peppa D, Schurich A, Khanna P, Singh H, Cheng Y, Rosenberg W, Dusheiko G, Gilson R, Chinaleong J, Kennedy P, Maini M Upregulation of the tim-3/galectin-9 pathway of T cell exhaustion in chronic hepatitis B virus infection. PLoS ONE, 2012-10-24;7(10):e47648. 2012-10-24 [PMID: 23112829] (Flow Cytometry, Human)
• Tandon , Ravi, Giret , Maria T, Sengupta , Devi, York , Vanessa, Wiznia , Andrew A, Rosenberg , Michael, Kallas , Esper G, Ndhlovu , Lishomwa, Nixon , Douglas Age-related expansion of Tim-3 expressing T cells in vertically HIV-1 infected children. PLoS ONE, 2012-09-24;7(9):e45733. 2012-09-24 [PMID: 23029209] (Flow Cytometry, Human)
• Sakhdari A, Mujib S, Vali B Tim-3 Negatively Regulates Cytotoxicity in Exhausted CD8(+) T Cells in HIV Infection. PLoS ONE, 2012-07-05;7(7):e40146. 2012-07-05 [PMID: 22792231] (Flow Cytometry, Human)
• Mujib S, Jones RB, Lo C, Aidarus N, Clayton K, Sakhdari A, Benko E, Kovacs C, Ostrowski MA Antigen-independent induction of Tim-3 expression on human T cells by the common gamma-chain cytokines IL-2, IL-7, IL-15, and IL-21 is associated with proliferation and is dependent on the phosphoinositide 3-kinase pathway. J. Immunol., 2012-03-14;188(8):3745-56. 2012-03-14 [PMID: 22422881] (Flow Cytometry, Human)
• Baitsch L, Legat A, Barba L, Fuertes Marraco SA, Rivals JP, Baumgaertner P, Christiansen-Jucht C, Bouzourene H, Rimoldi D, Pircher H, Rufer N, Matter M, Michielin O, Speiser DE Extended co-expression of inhibitory receptors by human CD8 T-cells depending on differentiation, antigen-specificity and anatomical localization. PLoS ONE, 2012-02-08;7(2):e30852. 2012-02-08 [PMID: 22347406] (Flow Cytometry, Human)
• Antonelli LR, Mahnke Y, Hodge JN, Porter BO, Barber DL, DerSimonian R, Greenwald JH, Roby G, Mican J, Sher A, Roederer M, Sereti I Elevated frequencies of highly activated CD4+ T cells in HIV+ patients developing immune reconstitution inflammatory syndrome. Blood, 2010-07-26;116(19):3818-27. 2010-07-26 [PMID: 20660788] (Flow Cytometry, Human)
• Idali F, Wahlstrom J, Dahlberg B, Khademi M, Olsson T, Eklund A, Grunewald J Altered expression of T cell immunoglobulin-mucin (TIM) molecules in bronchoalveolar lavage CD4+ T cells in sarcoidosis. Respir Res, 2009-05-29;10(0):42. 2009-05-29 [PMID: 19480659] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: HAVCR2
• Uniprot:
  • Q8TDQ0.2()